Psychotic issues are widespread and disabling psychological circumstances. The relative significance of immune-related mechanisms in psychotic issues stays topic of debate. Right here, we current a large-scale retrospective research of blood and cerebrospinal fluid (CSF) immune cell profiles of psychosis spectrum sufferers. We carried out primary CSF evaluation and multi-dimensional movement cytometry of CSF and blood cells from 59 sufferers with main psychotic issues (F20, F22, F23, and F25) compared to inflammatory (49 RRMS and 16 NMDARE sufferers) and non-inflammatory controls (52 IIH sufferers).
We replicated the recognized growth of monocytes within the blood of psychosis spectrum sufferers, that we recognized to preferentially have an effect on classical monocytes. Within the CSF, we discovered a relative shift from lymphocytes to monocytes, elevated protein ranges, and proof of blood-brain barrier disruption in psychosis. Actually, these CSF options confidently distinguished autoimmune encephalitis from psychosis regardless of comparable (preliminary) medical options.
We then constructed machine studying fashions incorporating blood and CSF parameters and demonstrated their superior capacity to distinguish psychosis from non-inflammatory controls in comparison with particular person parameters. Multi-dimensional and multi-compartment immune cell signatures can thus help the prognosis of psychosis spectrum issues with the potential to speed up prognosis and initiation of remedy.
Growth of a Particular Anti-capsid Antibody- and Magnetic Bead-Primarily based Immunoassay to Detect Human Norovirus Particles in Stool Samples and Spiked Mussels through Circulate Cytometry
Human noroviruses impose a substantial well being burden globally. Right here, a movement cytometry strategy designed for his or her detection in organic waste and meals samples was developed utilizing antibody-coated magnetic beads. Antipeptide antibodies in opposition to murine norovirus and numerous human norovirus genotypes had been generated for seize and coated onto magnetic beads. A movement cytometry assay was then carried out to detect bead-bound human norovirus GI.three in affected person stool samples and in norovirus-spiked mussel digestive tissues.
The detection restrict for stool samples was 105 gc/mL, thus bettering detection limits of commercially accessible norovirus prognosis fast kits of 100-fold; the detection restrict in spiked mussels nevertheless was ten-fold increased than in stool samples. Additional assays confirmed a lower in fluorescence depth for heat- or UV-inactivated virus particles. General, we exhibit the appliance of a movement cytometry strategy for direct detection of small non-enveloped virus particles comparable to noroviruses. An adaptation of the expertise to routine diagnostics has the potential to contribute a fast and delicate device to norovirus outbreak investigations. Additional enhancements to the tactic, notably lowering the detection restrict of the strategy, could permit the evaluation of naturally contaminated meals and environmental samples.
Single-reaction multi-antigen serological take a look at for complete analysis of SARS-CoV-2 sufferers by movement cytometry
Right here we describe a brand new, easy, extremely multiplexed serological take a look at that generates a extra full image of seroconversion than single antigen-based assays. Circulate cytometry is used to detect a number of immunoglobulin isotypes binding to 4 SARS-CoV-2 antigens: the Spike glycoprotein, its RBD fragment (the key goal for neutralising antibodies), the nucleocapsid protein and the principle cysteine-like protease in a single response. Till now, most diagnostic serological assessments measured antibodies to just one antigen and in some laboratory-confirmed sufferers no SARS-CoV-2-specific antibodies may very well be detected.
Our information reveal that whereas most sufferers reply in opposition to all of the viral antigens examined, others present a marked bias to make antibodies in opposition to both proteins uncovered on the viral particle or these launched after mobile an infection. With this assay, it was doable to discriminate between sufferers and wholesome controls with 100% confidence. Analysing the response of a number of immunoglobulin isotypes to the 4 antigens together can also assist to ascertain a correlation with the severity diploma of illness. A extra detailed description of the immune responses of various sufferers to SARS-CoV-2 virus may present perception into the big selection of medical shows of COVID-19. This text is protected by copyright. All rights reserved.
Progressive use of multispectral imaging movement cytometry in numerous analysis areas
Multi-spectral imaging movement cytometry (MIFC) has develop into one of the vital highly effective applied sciences for investigating common analytics, molecular and cell biology, biotechnology, medication, and associated fields. It combines the capabilities of the morphometric and photometric evaluation of single cells and micrometer-sized particles in flux with regard to 1000’s of occasions. It has develop into the device of selection for a variety of analysis and medical purposes. By combining the options of movement cytometry and fluorescence microscopy, it provides researchers the power to couple the spatial decision of multicolour photographs of cells and organelles with the simultaneous evaluation of a lot of occasions in a single system.
OCOA06230-20UG - GSG1L Protein Lysate |
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OCOA06230-20UG | Aviva Systems Biology | 20ug | EUR 169 |
Human GSG1L Over-expressing Stable Cell Line |
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ABC-X1328 | AcceGen | 1 vial | Ask for price |
Description: Gentaur can provide custom lentiviral constructs expressing any genes of interest as long as it is less than about 3 kb. Lentiviral technology enables us to efficiently generate stable expression lines which are then selected for moderate or high expressers, depending on the experimental requirements. If you are interested in specific lentiviral DNA constructs or have further questions, please contact us to discuss the details. GSG1L GSG1-like [ Homo sapiens ] http://www.ncbi.nlm.nih.gov/gene/146395 |
NEU3 Over-expression Lysate |
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GWB-DL2468 | GenWay Biotech | 0.1 mg | Ask for price |
Protein expression |
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CRO-4-Ind | Jotbody | each | Ask for price |
YLEX Expression Kit |
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FYY201-1KT | Yeastern Biotech | 1 kit | Ask for price |
RFP expression Adenovirus, in vivo ready |
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AVP001-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made RFP expression adenovirus, provided in PBS solution. |
CFP expression Adenovirus, in vivo ready |
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AVP002-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made CFP expression adenovirus, provided in PBS solution. |
GFP expression Adenovirus, in vivo ready |
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AVP011-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made GFP expression adenovirus, provided in PBS solution. |
YFP expression Adenovirus, in vivo ready |
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AVP012-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made YFP expression adenovirus, provided in PBS solution. |
BFP expression Adenovirus, in vivo ready |
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AVP017-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made BFP expression adenovirus, provided in PBS solution. |
EP Reagent Biuret Reagent - 100ML |
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1011601 | Scientific Laboratory Supplies | 1L | EUR 59.4 |
293 Expression MAX-1 |
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EXP-711-11 | ACROBIOSYSTEMS | 1ml | EUR 214 |
Description: 293 Expression MAX-1 is expressed from - -. |
PEROXIDE BLOCKING REAGENT Reagent |
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GWB-Q00308 | GenWay Biotech | 50 ml | Ask for price |
CNGA2 Expression vector |
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60642 | BPS Bioscience | 20 µg | EUR 900 |
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells. |
CNGA2 Expression vector |
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GWB-PSFA61 | GenWay Biotech | 20ug | Ask for price |
EP Reagent Iodoplatinate Reagent - 200ML |
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1046300 | Scientific Laboratory Supplies | 200ML | EUR 502.2 |
EP Reagent Molybdovanadic Reagent - 100ML |
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1056700 | Scientific Laboratory Supplies | 100ML | EUR 59.4 |
LEXSYcon2.1 Expression Kit |
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EGE-1310ble | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
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EGE-1310bsd | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
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EGE-1310hyg | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
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EGE-1310neo | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
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EGE-1310pac | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
LEXSYcon2.1 Expression Kit |
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EGE-1310sat | Jena Bioscience GmbH | 1Kit | EUR 1296.1 |
Description: for constitutive cytosolic or secretory protein expression |
pYLEX1 - Expression Vector |
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FYY203-5MG | Yeastern Biotech | 5mg | Ask for price |
h OCT4 expression Adenovirus, in vivo ready |
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AVP013-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application. |
h SOX2 expression Adenovirus, in vivo ready |
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AVP014-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application. |
Blunt E1 Expression Kit |
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abx098098-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E2 Expression Kit |
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abx098873-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E1 Expression Kit |
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abx098098-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E1 Expression Kit |
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abx098098-1ml | Abbexa | 1 ml | Ask for price |
Blunt E1 Expression Kit |
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abx098098-200l | Abbexa | 200 µl | Ask for price |
Blunt E2 Expression Kit |
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abx098873-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E2 Expression Kit |
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abx098873-1ml | Abbexa | 1 ml | Ask for price |
Blunt E2 Expression Kit |
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abx098873-200l | Abbexa | 200 µl | Ask for price |
RFP expression Adenovirus |
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AVP001 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made RFP expression adenovirus, provided in DMEM medium. |
CFP expression Adenovirus |
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AVP002 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made CFP expression adenovirus, provided in DMEM medium. |
GFP expression Adenovirus |
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AVP011 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made GFP expression adenovirus, provided in DMEM medium. |
YFP expression Adenovirus |
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AVP012 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made YFP expression adenovirus, provided in DMEM medium. |
BFP expression Adenovirus |
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AVP017 | GenTarget | 1x109 IFU/ml x 200ul | EUR 418.8 |
Description: pre-made BFP expression adenovirus, provided in DMEM medium. |
RPL21_17_477 Over-expression Lysate Product |
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GWB-8BD971 | GenWay Biotech | 0.1 mg | Ask for price |
h LIN28 expression Adenovirus, in vivo ready |
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AVP015-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 852 |
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application. |
LEXSinduce3 Expression Kit |
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EGE-1410ble | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
LEXSinduce3 Expression Kit |
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EGE-1410blecherry | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
LEXSinduce3 Expression Kit |
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EGE-1410neo | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
LEXSinduce4 Expression Kit |
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EGE-1420blecherry | Jena Bioscience GmbH | 1Kit | EUR 3037.6 |
Description: for inducible cytosolic or secretory protein expression |
EP Reagent Methoxyphenylacetic Reagent - 100ML |
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1053601 | Scientific Laboratory Supplies | 100ML | EUR 490.05 |
F8 Over-expression Lysate Product |
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GWB-4223CC | GenWay Biotech | 0.1 mg | Ask for price |
AR Over-expression Lysate Product |
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GWB-524326 | GenWay Biotech | 0.1 mg | Ask for price |
MB Over-expression Lysate Product |
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GWB-543EE8 | GenWay Biotech | 0.1 mg | Ask for price |
C5 Over-expression Lysate Product |
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GWB-611C50 | GenWay Biotech | 0.1 mg | Ask for price |
F7 Over-expression Lysate Product |
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GWB-2B9655 | GenWay Biotech | 0.1 mg | Ask for price |
HP Over-expression Lysate Product |
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GWB-19D90B | GenWay Biotech | 0.1 mg | Ask for price |
C9 Over-expression Lysate Product |
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GWB-A98C50 | GenWay Biotech | 0.1 mg | Ask for price |
GK Over-expression Lysate Product |
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GWB-ABEC99 | GenWay Biotech | 0.1 mg | Ask for price |
C6 Over-expression Lysate Product |
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GWB-4CD0B5 | GenWay Biotech | 0.1 mg | Ask for price |
T Over-expression Lysate Product |
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GWB-4D17A7 | GenWay Biotech | 0.1 mg | Ask for price |
C2 Over-expression Lysate Product |
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GWB-69AF56 | GenWay Biotech | 0.1 mg | Ask for price |
C7 Over-expression Lysate Product |
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GWB-6F310B | GenWay Biotech | 0.1 mg | Ask for price |
GC Over-expression Lysate Product |
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GWB-6FD425 | GenWay Biotech | 0.1 mg | Ask for price |
F2 Over-expression Lysate Product |
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GWB-74B4A0 | GenWay Biotech | 0.1 mg | Ask for price |
GK Over-expression Lysate Product |
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GWB-799C20 | GenWay Biotech | 0.1 mg | Ask for price |
F9 Over-expression Lysate Product |
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GWB-7A1142 | GenWay Biotech | 0.1 mg | Ask for price |
TF Over-expression Lysate Product |
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GWB-FDE7E0 | GenWay Biotech | 0.1 mg | Ask for price |
FH Over-expression Lysate Product |
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GWB-B00EEC | GenWay Biotech | 0.1 mg | Ask for price |
KL Over-expression Lysate Product |
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GWB-EF6FA2 | GenWay Biotech | 0.1 mg | Ask for price |
MB Over-expression Lysate Product |
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GWB-DDB100 | GenWay Biotech | 0.1 mg | Ask for price |
XG Over-expression Lysate Product |
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GWB-C19FF5 | GenWay Biotech | 0.1 mg | Ask for price |
PC Over-expression Lysate Product |
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GWB-C40C56 | GenWay Biotech | 0.1 mg | Ask for price |
Rac1 Expression Vector Set |
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STA-454 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant. |
RhoA Expression Vector Set |
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STA-456 | Cell Biolabs | 1 kit | EUR 1765.2 |
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant. |
CRX Over-expression Lysate Product |
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GWB-372B50 | GenWay Biotech | 0.1 mg | Ask for price |
FST Over-expression Lysate Product |
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GWB-3758FE | GenWay Biotech | 0.1 mg | Ask for price |
PNN Over-expression Lysate Product |
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GWB-379ABB | GenWay Biotech | 0.1 mg | Ask for price |
CRK Over-expression Lysate Product |
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GWB-37B7E4 | GenWay Biotech | 0.1 mg | Ask for price |
B2M Over-expression Lysate Product |
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GWB-39B4E3 | GenWay Biotech | 0.1 mg | Ask for price |
NGB Over-expression Lysate Product |
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GWB-39F54D | GenWay Biotech | 0.1 mg | Ask for price |
KIN Over-expression Lysate Product |
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GWB-3ABEBE | GenWay Biotech | 0.1 mg | Ask for price |
OXT Over-expression Lysate Product |
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GWB-3B1FD3 | GenWay Biotech | 0.1 mg | Ask for price |
PXK Over-expression Lysate Product |
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GWB-3C8EA1 | GenWay Biotech | 0.1 mg | Ask for price |
ALB Over-expression Lysate Product |
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GWB-3E03B0 | GenWay Biotech | 0.1 mg | Ask for price |
IL9 Over-expression Lysate Product |
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GWB-3E3A84 | GenWay Biotech | 0.1 mg | Ask for price |
HLA Over-expression Lysate Product |
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GWB-3E6FB0 | GenWay Biotech | 0.1 mg | Ask for price |
SPR Over-expression Lysate Product |
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GWB-3FACD1 | GenWay Biotech | 0.1 mg | Ask for price |
SPN Over-expression Lysate Product |
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GWB-404D95 | GenWay Biotech | 0.1 mg | Ask for price |
AIP Over-expression Lysate Product |
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GWB-40DF2C | GenWay Biotech | 0.1 mg | Ask for price |
CSK Over-expression Lysate Product |
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GWB-415B1A | GenWay Biotech | 0.1 mg | Ask for price |
WT1 Over-expression Lysate Product |
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GWB-41C1E8 | GenWay Biotech | 0.1 mg | Ask for price |
C8B Over-expression Lysate Product |
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GWB-41E9AB | GenWay Biotech | 0.1 mg | Ask for price |
HDC Over-expression Lysate Product |
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GWB-4302B9 | GenWay Biotech | 0.1 mg | Ask for price |
TNF Over-expression Lysate Product |
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GWB-448BBB | GenWay Biotech | 0.1 mg | Ask for price |
PNP Over-expression Lysate Product |
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GWB-4538EC | GenWay Biotech | 0.1 mg | Ask for price |
MUT Over-expression Lysate Product |
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GWB-455C37 | GenWay Biotech | 0.1 mg | Ask for price |
CFP Over-expression Lysate Product |
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GWB-4575E9 | GenWay Biotech | 0.1 mg | Ask for price |
HPN Over-expression Lysate Product |
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GWB-4602DE | GenWay Biotech | 0.1 mg | Ask for price |
C1D Over-expression Lysate Product |
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GWB-461FBD | GenWay Biotech | 0.1 mg | Ask for price |
PIP Over-expression Lysate Product |
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GWB-4782A0 | GenWay Biotech | 0.1 mg | Ask for price |
SF1 Over-expression Lysate Product |
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GWB-47A9D3 | GenWay Biotech | 0.1 mg | Ask for price |
DDO Over-expression Lysate Product |
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GWB-481A81 | GenWay Biotech | 0.1 mg | Ask for price |
LYZ Over-expression Lysate Product |
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GWB-4830E7 | GenWay Biotech | 0.1 mg | Ask for price |
PAM Over-expression Lysate Product |
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GWB-48AFF2 | GenWay Biotech | 0.1 mg | Ask for price |
DCN Over-expression Lysate Product |
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GWB-4AB343 | GenWay Biotech | 0.1 mg | Ask for price |
MVD Over-expression Lysate Product |
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GWB-4B1DAE | GenWay Biotech | 0.1 mg | Ask for price |
CPM Over-expression Lysate Product |
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GWB-4B1F3E | GenWay Biotech | 0.1 mg | Ask for price |
REN Over-expression Lysate Product |
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GWB-4B825B | GenWay Biotech | 0.1 mg | Ask for price |
HRK Over-expression Lysate Product |
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GWB-4BB955 | GenWay Biotech | 0.1 mg | Ask for price |
SHD Over-expression Lysate Product |
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GWB-4BFEE6 | GenWay Biotech | 0.1 mg | Ask for price |
GH1 Over-expression Lysate Product |
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GWB-4F3DD0 | GenWay Biotech | 0.1 mg | Ask for price |
IVD Over-expression Lysate Product |
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GWB-4F555F | GenWay Biotech | 0.1 mg | Ask for price |
SLA Over-expression Lysate Product |
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GWB-4F6FFE | GenWay Biotech | 0.1 mg | Ask for price |
AK4 Over-expression Lysate Product |
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GWB-4FADC0 | GenWay Biotech | 0.1 mg | Ask for price |
BRE Over-expression Lysate Product |
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GWB-4FC6EC | GenWay Biotech | 0.1 mg | Ask for price |
MOS Over-expression Lysate Product |
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GWB-503237 | GenWay Biotech | 0.1 mg | Ask for price |
ERF Over-expression Lysate Product |
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GWB-5242BB | GenWay Biotech | 0.1 mg | Ask for price |
HFE Over-expression Lysate Product |
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GWB-5243B9 | GenWay Biotech | 0.1 mg | Ask for price |
TEK Over-expression Lysate Product |
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GWB-533C4C | GenWay Biotech | 0.1 mg | Ask for price |
MIA Over-expression Lysate Product |
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GWB-534100 | GenWay Biotech | 0.1 mg | Ask for price |
IDE Over-expression Lysate Product |
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GWB-5367D7 | GenWay Biotech | 0.1 mg | Ask for price |
AFP Over-expression Lysate Product |
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GWB-536D51 | GenWay Biotech | 0.1 mg | Ask for price |
RDX Over-expression Lysate Product |
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GWB-53CF80 | GenWay Biotech | 0.1 mg | Ask for price |
ILK Over-expression Lysate Product |
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GWB-54E227 | GenWay Biotech | 0.1 mg | Ask for price |
SFN Over-expression Lysate Product |
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GWB-55C5F7 | GenWay Biotech | 0.1 mg | Ask for price |
BPI Over-expression Lysate Product |
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GWB-55FF0C | GenWay Biotech | 0.1 mg | Ask for price |
MAX Over-expression Lysate Product |
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GWB-56E43C | GenWay Biotech | 0.1 mg | Ask for price |
TAZ Over-expression Lysate Product |
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GWB-572853 | GenWay Biotech | 0.1 mg | Ask for price |
MLN Over-expression Lysate Product |
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GWB-576AA3 | GenWay Biotech | 0.1 mg | Ask for price |
CA2 Over-expression Lysate Product |
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GWB-578B82 | GenWay Biotech | 0.1 mg | Ask for price |
GIP Over-expression Lysate Product |
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GWB-57B640 | GenWay Biotech | 0.1 mg | Ask for price |
MKX Over-expression Lysate Product |
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GWB-57FD34 | GenWay Biotech | 0.1 mg | Ask for price |
FAS Over-expression Lysate Product |
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GWB-586DE6 | GenWay Biotech | 0.1 mg | Ask for price |
ADK Over-expression Lysate Product |
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GWB-590E04 | GenWay Biotech | 0.1 mg | Ask for price |
CAT Over-expression Lysate Product |
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GWB-59B4AF | GenWay Biotech | 0.1 mg | Ask for price |
AES Over-expression Lysate Product |
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GWB-5A4211 | GenWay Biotech | 0.1 mg | Ask for price |
GRP Over-expression Lysate Product |
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GWB-5A56CC | GenWay Biotech | 0.1 mg | Ask for price |
C8A Over-expression Lysate Product |
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GWB-5AD920 | GenWay Biotech | 0.1 mg | Ask for price |
ME2 Over-expression Lysate Product |
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GWB-5B40C3 | GenWay Biotech | 0.1 mg | Ask for price |
TTK Over-expression Lysate Product |
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GWB-5B4DBF | GenWay Biotech | 0.1 mg | Ask for price |
HGF Over-expression Lysate Product |
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GWB-5CC3A0 | GenWay Biotech | 0.1 mg | Ask for price |
This supplies the chance to visually verify findings and gather novel information that might in any other case be harder to acquire. This has led many researchers to design modern assays to realize new perception into essential analysis questions. So far, it has been efficiently used to check cell morphology, floor and nuclear protein co-localization, protein-protein interactions, cell signaling, cell cycle, cell dying, and cytotoxicity, intracellular calcium, drug uptake, pathogen internalization, and different purposes. Herein we describe a number of the current advances within the discipline of multiparametric imaging movement cytometry strategies in numerous analysis areas.