Cat # 86057-3
Column and bead-based separation methods are highly dependent on the speed and ease of affinity binding systems. Ligands such as streptavidin, antibodies, and lectins are used both to capture specifically labelled targets and for the isolation of cells and biomolecules that naturally express the binding partner of the ligand. The unique saccharide-binding properties of plant lectins, such as concanavalin A (Con A), have made them useful for labelling and isolating glycan and glycoprotein presenting cells in serum and cell lysate. In addition, lectins have been used in cell adhesion studies, to affect lymphocyte activation, and to explore carbohydrate-based therapies.
Our Con A-coated BioMag Plus microparticles provide a convenient means to isolate mannosyl- and glycosyl-containing glycoproteins and polysaccharides from serum or cell lysate, or to investigate other lectin/glycan mediated processes. The BioMag Plus magnetic particle format provides a large surface area and enables easy and efficient separations.
Requires: Cold Pack
Glycan Alternative, Glycoprotein Isolation, CUT & RUN ChIP [see References]
5 mg / ml
10 mM PBS with 0.1% sodium azide