The intestine related lymphoid tissue (GALT) effector websites play a vital position on the pathogenesis of many immune-mediated gastrointestinal ailments. The lymphocytes at these effector websites are principally T cells which current necessary morphological, phenotypical and purposeful variations. Circulation cytometry (FC) is likely one of the mostly used methods to characterize intestinal lymphocytes in human and animal fashions. Printed research with a deal with canine for intraepithelial lymphocytes (IEL) immunophenotyping exist in very restricted numbers.
Furthermore, no lamina propria lymphocytes (LPL) isolation protocols within the canine species have been described for FC analysis. Along with immune intestinal dysregulation, imbalances within the peripheral blood immune system have been described in each human and animal gastrointestinal issues. The goal of this examine was to supply a protocol for canine IEL and LPL isolation for FC immunophenotyping of T cells subsets. Particularly, T helper, T cytotoxic, activated Th and Tc lymphocytes, regulatory, double unfavorable, double optimistic, IFN-γ and IL-Four producing T cells, and to match their respective populations between these effector websites and with the blood stream compartment in wholesome canine.
The potential relationship of those cells distributions with age, intercourse and breed was additionally evaluated. This examine included sixteen wholesome canine of various sexes and breeds with a imply age of 4.55 ± 2.93 years previous. The chosen protocols for the three immune compartments confirmed correct cell yield, purity, viability, and the absence of phenotypic and purposeful disturbances. Histologically, an satisfactory separation of the duodenal epithelium from the lamina propria was additionally noticed.
All of the proposed T cells subsets have been recognized within the three immune compartments studied, exhibiting some statistically vital variations of their distributions at these areas that spotlight the significance of their particular person analysis. This examine offers an satisfactory methodology for canine small gut IEL and LPL isolation for FC immunophenotyping and is vital for future research on the gastrointestinal immune system related to completely different canine ailments.
Circulation Cytometry Detection of Sperm DNA Fragmentation and Apoptotic Markers within the Semen of Infertile Males
The impact of sperm molecular defects on fertilization and being pregnant end result after assisted reproductive remedy (ART) is extensively documented by each analysis and medical societies. Sperm DNA fragmentation and irregular chromatin condensation symbolize essential causes of male infertility. Superior androgenic methods for precisely figuring out molecular defects assist in deciding on an applicable therapy technique. Moreover, particular markers of apoptosis are more and more necessary in predicting male infertility. The power of circulation cytometry to estimate the amount of sperm with DNA fragmentation or injury and multifactor measurements in immotile sperm have made this developed approach important in fertility facilities.
The examine is geared toward assessing the extent of DNA fragmentation and apoptosis by measuring circulation cytometry utilizing new methods. Circulation cytometry evaluation revealed a various diploma of DNA injury. It was in a position to quantify the diploma of impairment even in samples with minimal DNA fragmentation. DNA injury was noticed even in samples that have been thought of regular with a routine semen evaluation. Circulation cytometry was delicate to adjustments in sperm apoptosis. Elevated p53 exercise ranges have been related to excessive DNA fragmentation. In the meantime, B-cell lymphoma 2 (Bcl-2) actions confirmed a unique sample. In conclusion, circulation cytometry for sperm DNA fragmentation and markers of apoptosis could be a priceless instrument in assisted reproductive facilities.
Transformation of Multicolour Circulation Cytometry knowledge with OTflow prevents deceptive multivariate evaluation (MVA) outcomes and incorrect immunological conclusions
The speedy evolution of the circulation cytometry subject, at present permitting the measurement of 30-50 parameters per cell, has led to a marked enhance in deep multivariate info. Handbook gating is inadequate to extract all this info. Due to this fact, multivariate evaluation (MVA) strategies have been developed to extract info and effectively analyse the high-density multicolour circulation cytometry (MFC) knowledge. To help interpretation, MFC knowledge are sometimes logarithmically reworked earlier than MVA.
We studied the implications of various transformations of circulation cytometry knowledge in datasets containing unfavorable intensities attributable to background subtractions and spreading error, as logarithmic transformation of unfavorable knowledge is inconceivable. Transformations akin to logicle or hyperbolic arcsine transformations permit linearity round zero, whereas larger (optimistic and unfavorable) intensities are logarithmically reworked. To outline the linear vary, a parameter (or cofactor) have to be chosen.
We present how the chosen transformation parameter has nice impression on the MVA outcomes. In some instances, peak-splitting is noticed, producing two distributions round zero in an precise homogeneous inhabitants. This can be misinterpreted because the presence of a number of cell populations. Furthermore, when performing arbitrary transformation earlier than MVA evaluation, biologically related and statistically vital info may be missed. We current a brand new algorithm, Optimum Transformation for circulation cytometry knowledge (OTflow), which makes use of numerous statistical strategies to optimally select the parameter of the transformation and forestall artifacts akin to peak splitting.
 Human Over-expression Lysate) LITAF (NM_001136472) Human Over-expression Lysate |
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| E45H01846-2 | EnoGene | each | EUR 395 |
 (NM_016132) Human Over-expression Lysate) Myelin expression factor 2 (MYEF2) (NM_016132) Human Over-expression Lysate |
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| LS050804 | BosterBio | 100ug | EUR 960 |
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Description: Transient overexpression lysate of myelin expression factor 2 (MYEF2) |
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Myelin expression factor 2 (MYEF2) (NM_016132) Human Over-expression Lysate |
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| E45H09950-2 | EnoGene | each | EUR 395 |
 baculoCOMPLETE protein expression kit |
|||
| 400100 | Oxford Expression Technologies | 5 reactions | EUR 621.81 |
 baculoCOMPLETE protein expression kit + baculoQUANT all-in-one |
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| 400101 | Oxford Expression Technologies | 5 + 100 reactions | EUR 1009.89 |
 Human LITAF Protein Lysate |
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| MBS8414542-002mg | MyBiosource | 0.02mg | EUR 365 |
Human LITAF Protein Lysate |
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| MBS8414542-5x002mg | MyBiosource | 5x0.02mg | EUR 1410 |
 Human LITAF Protein Lysate 20ug |
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| IHULITAFPLLY20UG | Innovative research | each | EUR 213 |
|
Description: Human LITAF Protein Lysate 20ug |
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 OCOA12906-20UG - LITAF Protein Lysate |
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| OCOA12906-20UG | Aviva Systems Biology | 20ug | EUR 169 |
 OCOA19001-20UG - LITAF Protein Lysate |
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| OCOA19001-20UG | Aviva Systems Biology | 20ug | EUR 169 |
 OCOA19002-20UG - LITAF Protein Lysate |
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| OCOA19002-20UG | Aviva Systems Biology | 20ug | EUR 169 |
 EP Reagent Biuret Reagent - 100ML |
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| 1011601 | Scientific Laboratory Supplies | 1L | EUR 59.4 |
 PEROXIDE BLOCKING REAGENT Reagent |
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| GWB-Q00308 | GenWay Biotech | 50 ml | Ask for price |
 EP Reagent Iodoplatinate Reagent - 200ML |
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| 1046300 | Scientific Laboratory Supplies | 200ML | EUR 502.2 |
 EP Reagent Molybdovanadic Reagent - 100ML |
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| 1056700 | Scientific Laboratory Supplies | 100ML | EUR 59.4 |
 EP Reagent Methoxyphenylacetic Reagent - 100ML |
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| 1053601 | Scientific Laboratory Supplies | 100ML | EUR 490.05 |
 Protein expression |
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| CRO-4-Ind | Jotbody | each | Ask for price |
 EP Reagent Phosphomolybdotungstic Reagent - 100ML |
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| 1065000 | Scientific Laboratory Supplies | 100ML | EUR 226.8 |
 RFP expression Adenovirus, in vivo ready |
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| AVP001-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
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Description: pre-made RFP expression adenovirus, provided in PBS solution. |
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 CFP expression Adenovirus, in vivo ready |
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| AVP002-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
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Description: pre-made CFP expression adenovirus, provided in PBS solution. |
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 GFP expression Adenovirus, in vivo ready |
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| AVP011-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
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Description: pre-made GFP expression adenovirus, provided in PBS solution. |
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 YFP expression Adenovirus, in vivo ready |
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| AVP012-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
|
Description: pre-made YFP expression adenovirus, provided in PBS solution. |
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 BFP expression Adenovirus, in vivo ready |
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| AVP017-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
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Description: pre-made BFP expression adenovirus, provided in PBS solution. |
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 AVIDIN/BIOTIN BLOCKING REAGENT Reagent |
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| GWB-Q00289 | GenWay Biotech | 30 ml | Ask for price |
) Gordon-McLeod Reagent (Oxidase reagent) |
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| R026-100ML | EWC Diagnostics | 1 unit | EUR 7.76 |
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Description: Gordon-McLeod Reagent (Oxidase reagent) |
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) Oxidase Reagent (Gordon-Macleod Reagent) |
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| 76207 | Sisco Laboratories | 100 ml | EUR 3.38 |
|
Description: Part B |
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 YLEX Expression Kit |
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| FYY201-1KT | Yeastern Biotech | 1 kit | Ask for price |
 EP Reagent Sulfomolybdic Reagent R3 - 1L |
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| 1086500 | Scientific Laboratory Supplies | 1L | EUR 398.25 |
) TRIpure Reagent (same as Trizol Reagent) |
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| MBS356001-INQUIRE | MyBiosource | INQUIRE | Ask for price |
 h OCT4 expression Adenovirus, in vivo ready |
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| AVP013-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
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Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application. |
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 h SOX2 expression Adenovirus, in vivo ready |
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| AVP014-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
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Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application. |
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 293 Expression MAX-1 |
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| EXP-711-11 | ACROBIOSYSTEMS | 1ml | EUR 218 |
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Description: 293 Expression MAX-1 is expressed from - -. |
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 h LIN28 expression Adenovirus, in vivo ready |
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| AVP015-PBS | GenTarget | 1x1011 IFU/ml x 50ul | EUR 455 |
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Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application. |
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) Total RNA Isolation Reagent (Reagent Based) |
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| MBS402052-100mL | MyBiosource | 100mL | EUR 235 |
Total RNA Isolation Reagent (Reagent Based) |
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| MBS402052-4Reactions | MyBiosource | 4Reactions | EUR 165 |
) Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402050-100mL | MyBiosource | 100mL | EUR 255 |
Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402050-4Reactions | MyBiosource | 4Reactions | EUR 185 |
 CNGA2 Expression vector |
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| 60642 | BPS Bioscience | 20 µg | EUR 900 |
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Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells. |
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CNGA2 Expression vector |
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| GWB-PSFA61 | GenWay Biotech | 20ug | Ask for price |
 LEXSYcon2.1 Expression Kit |
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| EGE-1310ble | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
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Description: for constitutive cytosolic or secretory protein expression |
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LEXSYcon2.1 Expression Kit |
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| EGE-1310bsd | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
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Description: for constitutive cytosolic or secretory protein expression |
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LEXSYcon2.1 Expression Kit |
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| EGE-1310hyg | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
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Description: for constitutive cytosolic or secretory protein expression |
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LEXSYcon2.1 Expression Kit |
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| EGE-1310neo | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
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Description: for constitutive cytosolic or secretory protein expression |
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LEXSYcon2.1 Expression Kit |
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| EGE-1310pac | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
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Description: for constitutive cytosolic or secretory protein expression |
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LEXSYcon2.1 Expression Kit |
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| EGE-1310sat | Jena Bioscience GmbH | 1Kit | EUR 979.85 |
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Description: for constitutive cytosolic or secretory protein expression |
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 pYLEX1 - Expression Vector |
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| FYY203-5MG | Yeastern Biotech | 5mg | Ask for price |
 Blunt E1 Expression Kit |
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| abx098098-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E1 Expression Kit |
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| abx098098-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E1 Expression Kit |
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| abx098098-1ml | Abbexa | 1 ml | Ask for price |
Blunt E1 Expression Kit |
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| abx098098-200l | Abbexa | 200 µl | Ask for price |
 Blunt E2 Expression Kit |
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| abx098873-100l | Abbexa | 100 µl | EUR 712.5 |
Blunt E2 Expression Kit |
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| abx098873-10rxns | Abbexa | 10 rxns | EUR 560.4 |
Blunt E2 Expression Kit |
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| abx098873-1ml | Abbexa | 1 ml | Ask for price |
Blunt E2 Expression Kit |
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| abx098873-200l | Abbexa | 200 µl | Ask for price |
 RFP expression Adenovirus |
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| AVP001 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
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Description: pre-made RFP expression adenovirus, provided in DMEM medium. |
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 CFP expression Adenovirus |
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| AVP002 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
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Description: pre-made CFP expression adenovirus, provided in DMEM medium. |
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 GFP expression Adenovirus |
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| AVP011 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
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Description: pre-made GFP expression adenovirus, provided in DMEM medium. |
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 YFP expression Adenovirus |
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| AVP012 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
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Description: pre-made YFP expression adenovirus, provided in DMEM medium. |
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 BFP expression Adenovirus |
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| AVP017 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
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Description: pre-made BFP expression adenovirus, provided in DMEM medium. |
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 LEXSinduce3 Expression Kit |
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| EGE-1410ble | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
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Description: for inducible cytosolic or secretory protein expression |
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LEXSinduce3 Expression Kit |
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| EGE-1410blecherry | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
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Description: for inducible cytosolic or secretory protein expression |
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LEXSinduce3 Expression Kit |
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| EGE-1410neo | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
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Description: for inducible cytosolic or secretory protein expression |
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 LEXSinduce4 Expression Kit |
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| EGE-1420blecherry | Jena Bioscience GmbH | 1Kit | EUR 2296.43 |
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Description: for inducible cytosolic or secretory protein expression |
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 Rac1 Expression Vector Set |
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| STA-454 | Cell Biolabs | 1 kit | EUR 1765.2 |
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Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant. |
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 RhoA Expression Vector Set |
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| STA-456 | Cell Biolabs | 1 kit | EUR 1765.2 |
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Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant. |
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) Plant Total RNA Isolation Reagent (Reagent Based) |
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| MBS402053-100mL | MyBiosource | 100mL | EUR 245 |
Plant Total RNA Isolation Reagent (Reagent Based) |
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| MBS402053-4Reactions | MyBiosource | 4Reactions | EUR 165 |
 OPN expression inhibitor 1 |
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| HY-146064 | MedChemExpress | 10 mg | EUR 2272.76 |
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Description: OPN expression inhibitor 1 (Compound 11) is an osteopontin (OPN) expression inhibitor. OPN expression inhibitor 1 can be used for breast cancer research[1]. |
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 Cdc42 Expression Vector Set |
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| STA-455 | Cell Biolabs | 1 kit | EUR 1765.2 |
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Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. |
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OPN expression inhibitor 1 |
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| T62820-10mg | TargetMol Chemicals | 10mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
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OPN expression inhibitor 1 |
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| T62820-1g | TargetMol Chemicals | 1g | Ask for price |
|
Description: OPN expression inhibitor 1 |
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OPN expression inhibitor 1 |
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| T62820-1mg | TargetMol Chemicals | 1mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
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OPN expression inhibitor 1 |
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| T62820-50mg | TargetMol Chemicals | 50mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
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OPN expression inhibitor 1 |
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| T62820-5mg | TargetMol Chemicals | 5mg | Ask for price |
|
Description: OPN expression inhibitor 1 |
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) Plant Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402051-100mL | MyBiosource | 100mL | EUR 245 |
Plant Genomic DNA Isolation Reagent (Reagent Based) |
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| MBS402051-4Reactions | MyBiosource | 4Reactions | EUR 165 |
 h IL6 Expression Lentivirus |
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| LVP1144 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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 h CD4 Expression Lentivirus |
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| LVP1152 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker. |
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 h IL4 Expression Lentivirus |
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| LVP1161 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker. |
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 h IL7 Expression Lentivirus |
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| LVP1162 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
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Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker. |
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 h FAP Expression Lentivirus |
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| LVP1307 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: FAP, containing a RFP-Blasticidin dual selection marker. |
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 h CD1 Expression Lentivirus |
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| LVP1320 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
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Description: Over-expression lentivirus for human target: CD1, containing a RFP-Blasticidin dual selection marker. |
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 m FAP Expression Lentivirus |
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| LVP1326 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for mouse target: FAP, containing a RFP-Blasticidin dual selection marker. |
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 h VIM Expression Lentivirus |
|||
| LVP1357 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: VIM, containing a RFP-Blasticidin dual selection marker. |
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 h PXN Expression Lentivirus |
|||
| LVP1358 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: PXN, containing a RFP-Blasticidin dual selection marker. |
|||
 m CD8 Expression Lentivirus |
|||
| LVP1363 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for mouse target: CD8, containing a RFP-Blasticidin dual selection marker. |
|||
 h TPO Expression Lentivirus |
|||
| LVP1371 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: TPO, containing a RFP-Blasticidin dual selection marker. |
|||
 h WAS Expression Lentivirus |
|||
| LVP1429 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: WAS, containing a RFP-Blasticidin dual selection marker. |
|||
 m PGR Expression Lentivirus |
|||
| LVP1455 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for mouse target: PGR containing a RFP-Blasticidin dual selection marker. |
|||
 h MAG Expression Lentivirus |
|||
| LVP1485 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for human target: MAG, containing a RFP-Blasticidin dual selection marker. |
|||
 h FST Expression Lentivirus |
|||
| LVP1518 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
|
Description: Pre-made lentivirus express human FST gene, containing a RFP-Blasticidin dual selection marker. |
|||
 h IL2 Expression Lentivirus |
|||
| LVP293 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Lentivirus express a human IL2 gene with a Blasticidin-RFP fusion marker (Dual selection). |
|||
 h ADA Expression Lentivirus |
|||
| LVP923 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker. |
|||
 h PBK Expression Lentivirus |
|||
| LVP095 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made Lentivirus express human PBK gene, carrying a RFP-Blasticidin fusion marker (Dual selection). |
|||
 pAAV-MCS Expression Vector |
|||
| MBS169528-001mg | MyBiosource | 0.01mg | EUR 695 |
pAAV-MCS Expression Vector |
|||
| MBS169528-5x001mg | MyBiosource | 5x0.01mg | EUR 2955 |
pAAV-MCS Expression Vector |
|||
| VPK-410 | Cell Biolabs | 10 µg | EUR 444 |
) FlashBAC - Baculovirus Expression System (5 reactions) |
|||
| GWB-7676BB | GenWay Biotech | Kit | Ask for price |
) FlashBAC - Baculovirus Expression System (96 reactions) |
|||
| GWB-6C34E3 | GenWay Biotech | Kit | Ask for price |
) FlashBAC - Baculovirus Expression System(24 reactions) |
|||
| GWB-EB1FF8 | GenWay Biotech | Kit | Ask for price |
) ExoPure? Reagent (Overall Exosome Isolation, urine) |
|||
| M1003-25 | Biovision | each | EUR 992.4 |
ExoPure? Reagent (Overall Exosome Isolation, urine) |
|||
| M1003-50 | Biovision | each | EUR 1083.6 |
 h EGFR Expression Lentivirus |
|||
| LVP1142 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
|
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker. |
|||
 h CD8a Expression Lentivirus |
|||
| LVP1151 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
|
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker. |
|||
 h PIGF Expression Lentivirus |
|||
| LVP1154 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker. |
|||
 h BRAF Expression Lentivirus |
|||
| LVP1156 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker. |
|||
 h ROS1 Expression Lentivirus |
|||
| LVP1158 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker. |
|||
 h CD3E Expression Lentivirus |
|||
| LVP1163 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker. |
|||
 h KLK3 Expression Lentivirus |
|||
| LVP1171 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker. |
|||
 h NEK6 Expression Lentivirus |
|||
| LVP1182 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
|
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker. |
|||
 h NEK7 Expression Lentivirus |
|||
| LVP1183 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker. |
|||
 h STM2 Expression Lentivirus |
|||
| LVP1210 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: STM2, containing a RFP-Blasticidin dual selection marker. |
|||
 h CBX7 Expression Lentivirus |
|||
| LVP1308 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: CBX7, containing a RFP-Blasticidin dual selection marker. |
|||
 h ACE2 Expression Lentivirus |
|||
| LVP1310 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: ACE2, containing a RFP-Blasticidin dual selection marker. |
|||
 h Rab7 Expression Lentivirus |
|||
| LVP1315 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: Rab7, containing a RFP-Blasticidin dual selection marker. |
|||
 h Rab5 Expression Lentivirus |
|||
| LVP1316 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: Rab5, containing a RFP-Blasticidin dual selection marker. |
|||
 m ASPA Expression Lentivirus |
|||
| LVP1317 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for mouse target: ASPA, containing a RFP-Blasticidin dual selection marker. |
|||
 h CD44 Expression Lentivirus |
|||
| LVP1318 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: CD44, containing a RFP-Blasticidin dual selection marker. |
|||
 h MFN2 Expression Lentivirus |
|||
| LVP1327 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: MFN2, containing a RFP-Blasticidin dual selection marker. |
|||
 h ANO1 Expression Lentivirus |
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| LVP1344 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: ANO1 containing a RFP-Blasticidin dual selection marker. |
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 h FGL2 Expression Lentivirus |
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| LVP1346 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: FGL2, containing a RFP-Blasticidin dual selection marker. |
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 h CCR2 Expression Lentivirus |
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| LVP1354 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: CCR2, containing a RFP-Blasticidin dual selection marker. |
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 h NEFL Expression Lentivirus |
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| LVP1360 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: NEFL, containing a RFP-Blasticidin dual selection marker. |
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 h NOS2 Expression Lentivirus |
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| LVP1367 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: NOS2, containing a RFP-Blasticidin dual selection marker. |
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 h BMI1 Expression Lentivirus |
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| LVP1372 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: BMI1, containing a RFP-Blasticidin dual selection marker. |
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 h PSCA Expression Lentivirus |
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| LVP1374 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: PSCA, containing a RFP-Blasticidin dual selection marker. |
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 h POLQ Expression Lentivirus |
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| LVP1421 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: POLQ containing a RFP-Blasticidin dual selection marker. |
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 h ARSA Expression Lentivirus |
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| LVP1425 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: ARSA, containing a RFP-Blasticidin dual selection marker. |
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 h IDUA Expression Lentivirus |
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| LVP1426 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: IDUA, containing a RFP-Blasticidin dual selection marker. |
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 h SGSH Expression Lentivirus |
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| LVP1427 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: SGSH, containing a RFP-Blasticidin dual selection marker. |
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 h CYBB Expression Lentivirus |
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| LVP1430 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: CYBB, containing a RFP-Blasticidin dual selection marker. |
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 h CAV1 Expression Lentivirus |
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| LVP1431 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: CAV1, containing a RFP-Blasticidin dual selection marker. |
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 m INS2 Expression Lentivirus |
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| LVP1456 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for mouse target: INS2 containing a RFP-Blasticidin dual selection marker. |
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 h ROR1 Expression Lentivirus |
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| LVP1458 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for human target: ROR1, containing a RFP-Blasticidin dual selection marker. |
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 h DGKA Expression Lentivirus |
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| LVP1468 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for human target: DGKA, containing a RFP-Blasticidin dual selection marker. |
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 h REST_HA Expression Lentivirus |
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| LVP1475 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for human target: REST, containing a RFP-Blasticidin dual selection marker. |
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 h UBR7 Expression Lentivirus |
|||
| LVP1483 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 346.5 |
|
Description: Pre-made over-expression lentivirus for human target: UBR7, containing a RFP-Blasticidin dual selection marker. |
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 h MMP1 Expression Lentivirus |
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| LVP1516 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
|
Description: Pre-made lentivirus express human MMP1 gene, containing a RFP-Blasticidin dual selection marker. |
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 h BDNF Expression Lentivirus |
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| LVP1520 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
|
Description: Pre-made lentivirus express human BDNF gene, containing a RFP-Blasticidin dual selection marker. |
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 m CCL5 Expression Lentivirus |
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| LVP1619 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 455 |
|
Description: Pre-made lentivirus express mouse CCL5 gene, containing a RFP-Blasticidin dual selection marker. |
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 h TET2 Expression Lentivirus |
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| LVP875 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made lentivirus express human target: TET2 (tet methylcytosine dioxygenase 2). It contains a RFP-Blasticidin dual selection marker. |
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 h TET3 Expression Lentivirus |
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| LVP876 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for expressing human target: TET3 (tet methylcytosine dioxygenase 3), [alternative names: hCG_40738]. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_001287491.1 . It also contains a RFP-Blasticidin dual selection marker. |
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 h CDR1 Expression Lentivirus |
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| LVP917 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker. |
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 h CD47 Expression Lentivirus |
|||
| LVP918 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
|
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker. |
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 h ESR1 Expression Lentivirus |
|||
| LVP924 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker. |
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 r Syn1 Expression Lentivirus |
|||
| LVP925 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker. |
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 h NME4 Expression Lentivirus |
|||
| LVP932 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker. |
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 h OCT4 expression Adenovirus |
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| AVP013 | GenTarget | 1x109 IFU/ml x 200ul | EUR 206.5 |
|
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium. |
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 h SOX2 expression Adenovirus |
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| AVP014 | GenTarget | 1x109 IFU/ml x 50ul | EUR 206.5 |
|
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium. |
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 N.N.C.D.-reagent |
|||
| N632003 | Toronto Research Chemicals | 1g | EUR 1200 |
|
Description: 6035-19-4 |
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 H-Ras Expression Vector Set |
|||
| STA-457 | Cell Biolabs | 1 kit | EUR 1765.2 |
|
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant. |
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 h ZRSR2 Expression Lentivirus |
|||
| LVP1118 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for expressing human target: ZRSR2 (zinc finger CCCH-type, RNA binding motif and serine/arginine rich 2), [alternative names: U2AF1-RS2; U2AF1L2; U2AF1RS2; URP; ZC3H22]. The sub-cloned codon sequence is identical (100% match) to CDS region in NCBI ID: NM_005089. It also contains a RFP-Blasticidin dual selection marker. |
|||
 h HDAC1 Expression Lentivirus |
|||
| LVP1143 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
|||
 h VEGFB Expression Lentivirus |
|||
| LVP1146 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
|||
 h PARP1 Expression Lentivirus |
|||
| LVP1149 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker. |
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 h IL3RA Expression Lentivirus |
|||
| LVP1150 | GenTarget | 1x107 IFU/ml x 200ul | EUR 541.2 |
|
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker. |
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 h PTCH1 Expression Lentivirus |
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| LVP1155 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker. |
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 h SART3 Expression Lentivirus |
|||
| LVP1159 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker. |
|||
 h TIGIT Expression Lentivirus |
|||
| LVP1160 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker. |
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 h EDNRA Expression Lentivirus |
|||
| LVP1165 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker. |
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 h Foxp3 Expression Lentivirus |
|||
| LVP1172 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker. |
|||
 m RIKEN Expression Lentivirus |
|||
| LVP1174 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker. |
|||
 h APLP2 Expression Lentivirus |
|||
| LVP1177 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker. |
|||
 h PCSK9 Expression Lentivirus |
|||
| LVP1178 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker. |
|||
 h NTSR1 Expression Lentivirus |
|||
| LVP1179 | GenTarget | 1x107 IFU/ml x 200ul | EUR 276.5 |
|
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker. |
|||
 h FADS2 Expression Lentivirus |
|||
| LVP1319 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: FADS2, containing a RFP-Blasticidin dual selection marker. |
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 h OVOL2 Expression Lentivirus |
|||
| LVP1322 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: OVOL2, containing a RFP-Blasticidin dual selection marker. |
|||
 h APOA1 Expression Lentivirus |
|||
| LVP1324 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: APOA1, containing a RFP-Blasticidin dual selection marker. |
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 h SOX10 Expression Lentivirus |
|||
| LVP1325 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: SOX10, containing a RFP-Blasticidin dual selection marker. |
|||
 h P2RY1 Expression Lentivirus |
|||
| LVP1328 | GenTarget | 1x10^7 IFU/ml x 200ul | EUR 276.5 |
|
Description: Over-expression lentivirus for human target: P2RY1, containing a RFP-Blasticidin dual selection marker. |
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Arbitrary or unconsidered transformation can result in flawed conclusions for the MVA cluster strategies, dimensionality discount strategies and classification strategies. We advocate transformation of circulation cytometry knowledge through the use of OTflow-defined parameters estimated per channel, so as to forestall peak-splitting and different artifacts within the knowledge. This text is protected by copyright. All rights reserved.