Isolation and immunophenotyping by flow cytometry of canine peripheral blood and intraepithelial and lamina propria duodenal T lymphocytes

Isolation and immunophenotyping by flow cytometry of canine peripheral blood and intraepithelial and lamina propria duodenal T lymphocytes

The intestine related lymphoid tissue (GALT) effector websites play a vital position on the pathogenesis of many immune-mediated gastrointestinal ailments. The lymphocytes at these effector websites are principally T cells which current necessary morphological, phenotypical and purposeful variations. Circulation cytometry (FC) is likely one of the mostly used methods to characterize intestinal lymphocytes in human and animal fashions. Printed research with a deal with canine for intraepithelial lymphocytes (IEL) immunophenotyping exist in very restricted numbers.

Furthermore, no lamina propria lymphocytes (LPL) isolation protocols within the canine species have been described for FC analysis. Along with immune intestinal dysregulation, imbalances within the peripheral blood immune system have been described in each human and animal gastrointestinal issues. The goal of this examine was to supply a protocol for canine IEL and LPL isolation for FC immunophenotyping of T cells subsets. Particularly, T helper, T cytotoxic, activated Th and Tc lymphocytes, regulatory, double unfavorable, double optimistic, IFN-γ and IL-Four producing T cells, and to match their respective populations between these effector websites and with the blood stream compartment in wholesome canine.

The potential relationship of those cells distributions with age, intercourse and breed was additionally evaluated. This examine included sixteen wholesome canine of various sexes and breeds with a imply age of 4.55 ± 2.93 years previous. The chosen protocols for the three immune compartments confirmed correct cell yield, purity, viability, and the absence of phenotypic and purposeful disturbances. Histologically, an satisfactory separation of the duodenal epithelium from the lamina propria was additionally noticed.

All of the proposed T cells subsets have been recognized within the three immune compartments studied, exhibiting some statistically vital variations of their distributions at these areas that spotlight the significance of their particular person analysis. This examine offers an satisfactory methodology for canine small gut IEL and LPL isolation for FC immunophenotyping and is vital for future research on the gastrointestinal immune system related to completely different canine ailments.

Circulation Cytometry Detection of Sperm DNA Fragmentation and Apoptotic Markers within the Semen of Infertile Males

The impact of sperm molecular defects on fertilization and being pregnant end result after assisted reproductive remedy (ART) is extensively documented by each analysis and medical societies. Sperm DNA fragmentation and irregular chromatin condensation symbolize essential causes of male infertility. Superior androgenic methods for precisely figuring out molecular defects assist in deciding on an applicable therapy technique. Moreover, particular markers of apoptosis are more and more necessary in predicting male infertility. The power of circulation cytometry to estimate the amount of sperm with DNA fragmentation or injury and multifactor measurements in immotile sperm have made this developed approach important in fertility facilities.

Isolation and immunophenotyping by flow cytometry of canine peripheral blood and intraepithelial and lamina propria duodenal T lymphocytes

The examine is geared toward assessing the extent of DNA fragmentation and apoptosis by measuring circulation cytometry utilizing new methods. Circulation cytometry evaluation revealed a various diploma of DNA injury. It was in a position to quantify the diploma of impairment even in samples with minimal DNA fragmentation. DNA injury was noticed even in samples that have been thought of regular with a routine semen evaluation. Circulation cytometry was delicate to adjustments in sperm apoptosis. Elevated p53 exercise ranges have been related to excessive DNA fragmentation. In the meantime, B-cell lymphoma 2 (Bcl-2) actions confirmed a unique sample. In conclusion, circulation cytometry for sperm DNA fragmentation and markers of apoptosis could be a priceless instrument in assisted reproductive facilities.

Transformation of Multicolour Circulation Cytometry knowledge with OTflow prevents deceptive multivariate evaluation (MVA) outcomes and incorrect immunological conclusions

The speedy evolution of the circulation cytometry subject, at present permitting the measurement of 30-50 parameters per cell, has led to a marked enhance in deep multivariate info. Handbook gating is inadequate to extract all this info. Due to this fact, multivariate evaluation (MVA) strategies have been developed to extract info and effectively analyse the high-density multicolour circulation cytometry (MFC) knowledge. To help interpretation, MFC knowledge are sometimes logarithmically reworked earlier than MVA.
We studied the implications of various transformations of circulation cytometry knowledge in datasets containing unfavorable intensities attributable to background subtractions and spreading error, as logarithmic transformation of unfavorable knowledge is inconceivable. Transformations akin to logicle or hyperbolic arcsine transformations permit linearity round zero, whereas larger (optimistic and unfavorable) intensities are logarithmically reworked. To outline the linear vary, a parameter (or cofactor) have to be chosen.
We present how the chosen transformation parameter has nice impression on the MVA outcomes. In some instances, peak-splitting is noticed, producing two distributions round zero in an precise homogeneous inhabitants. This can be misinterpreted because the presence of a number of cell populations. Furthermore, when performing arbitrary transformation earlier than MVA evaluation, biologically related and statistically vital info may be missed. We current a brand new algorithm, Optimum Transformation for circulation cytometry knowledge (OTflow), which makes use of numerous statistical strategies to optimally select the parameter of the transformation and forestall artifacts akin to peak splitting.

LITAF Antibody

DF7357 200ul
EUR 304
Description: LITAF Antibody detects endogenous levels of total LITAF.

LITAF Antibody

1-CSB-PA012985ESR1HU
  • EUR 222.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LITAF. Recognizes LITAF from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

LITAF Antibody

1-CSB-PA012985GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against LITAF. Recognizes LITAF from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

LITAF siRNA

20-abx922642
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

LITAF siRNA

20-abx922643
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

LITAF antibody

22499-100ul 100ul
EUR 390

LITAF Antibody

32869-100ul 100ul
EUR 252

LITAF siRNA

20-abx902997
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

HEK-293T Telomerase Over-Expressing Cell Pellet

abx069991-1Pellet 1 Pellet
EUR 398

Anti-LITAF (2E12)

YF-MA16893 100 ug
EUR 363
Description: Mouse monoclonal to LITAF

LITAF Blocking Peptide

DF7357-BP 1mg
EUR 195

Anti-LITAF antibody

PAab04798 100 ug
EUR 355

LITAF cloning plasmid

CSB-CL859109HU-10ug 10ug
EUR 233
Description: A cloning plasmid for the LITAF gene.

LITAF Polyclonal Antibody

ES11138-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against LITAF from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

LITAF Polyclonal Antibody

ES11138-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against LITAF from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

LITAF Polyclonal Antibody

ABP59126-003ml 0.03ml
EUR 158
Description: A polyclonal antibody for detection of LITAF from Human, Mouse, Rat. This LITAF antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LITAF protein

LITAF Polyclonal Antibody

ABP59126-01ml 0.1ml
EUR 289
Description: A polyclonal antibody for detection of LITAF from Human, Mouse, Rat. This LITAF antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LITAF protein

LITAF Polyclonal Antibody

ABP59126-02ml 0.2ml
EUR 414
Description: A polyclonal antibody for detection of LITAF from Human, Mouse, Rat. This LITAF antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human LITAF protein

Anti-LITAF antibody

STJ27422 100 µl
EUR 277
Description: Lipopolysaccharide is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor-alpha (TNF-alpha) and other inflammatory mediators. This gene encodes lipopolysaccharide-induced TNF-alpha factor, which is a DNA-binding protein and can mediate the TNF-alpha expression by direct binding to the promoter region of the TNF-alpha gene. The transcription of this gene is induced by tumor suppressor p53 and has been implicated in the p53-induced apoptotic pathway. Mutations in this gene cause Charcot-Marie-Tooth disease type 1C (CMT1C) and may be involved in the carcinogenesis of extramammary Paget's disease (EMPD). Multiple alternatively spliced transcript variants have been found for this gene.

Polyclonal LITAF Antibody

APR17201G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LITAF . This antibody is tested and proven to work in the following applications:

Anti-LITAF antibody

STJ192296 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to LITAF

Anti-LITAF antibody

STJ71339 100 µg
EUR 359

anti- LITAF antibody

FNab04798 100µg
EUR 505.25
Description: Antibody raised against LITAF

LITAF Conjugated Antibody

C32869 100ul
EUR 397

Chymase reagent

30C-CP1129 5 units
EUR 2185
Description: Purified native Human Chymase reagent

Traut's Reagent

2330-1000
EUR 349

Traut's Reagent

2330-500
EUR 207

MTS Reagent

2808-1000
EUR 990

MTS Reagent

2808-250
EUR 365

MTT Reagent

2809-1G
EUR 180

MTT Reagent

2809-5G
EUR 544

Bradford reagent

BDE641 100ml
EUR 61.01

Beaucage reagent

HY-100951 10mM/1mL
EUR 126

BOP reagent

5-02141 25g Ask for price

BOP reagent

5-02142 100g Ask for price

Bluing Reagent

BRT030 30 ml
EUR 60

Bluing Reagent

BRT125 125 ml
EUR 63

Bluing Reagent

BRT3800 1 Gal.
EUR 184

Bluing Reagent

BRT500 500 ml
EUR 76

Bluing Reagent

BRT999 1000 ml
EUR 88

BOP reagent

A7015-100000 100 g
EUR 200
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

BOP reagent

A7015-25000 25 g
EUR 113
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

LITAF protein (His tag)

80R-2650 100 ug
EUR 322
Description: Purified recombinant Human LITAF protein (His tag)

Human LITAF ELISA KIT

ELI-12371h 96 Tests
EUR 824

Mouse Litaf ELISA KIT

ELI-12372m 96 Tests
EUR 865

LITAF ELISA KIT|Human

EF010691 96 Tests
EUR 689

Chicken LITAF ELISA KIT

ELI-43561c 96 Tests
EUR 928

LITAF Recombinant Protein (Human)

RP040795 100 ug Ask for price

LITAF Recombinant Protein (Rat)

RP208280 100 ug Ask for price

LITAF Recombinant Protein (Mouse)

RP147635 100 ug Ask for price

Mouse LITAF shRNA Plasmid

20-abx974878
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Rat LITAF shRNA Plasmid

20-abx986408
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human LITAF shRNA Plasmid

20-abx956322
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

HAMA blocking reagent

85R-1001 1 gram
EUR 1974
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA

HAMA blocking reagent

85R-1001P 1 gram
EUR 2190
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA

HAMA blocking reagent

85R-1003 1 gram
EUR 1974
Description: HAMA Blocking Reagent for use in immunoassays such as Rapid Tests

HAMA blocking reagent

85R-1014 50 mg
EUR 192
Description: HAMA blocking reagent for use in assays specific for clinical false positive samples

HAMA blocking reagent

85R-1025 50 mg
EUR 192
Description: HAMA blocking reagent for use in immunoassays

HAMA blocking reagent

85R-1026 50 mg
EUR 192
Description: HAMA blocking reagent for use in immunoassays

ExFect2000 Transfection Reagent

T202-01 0.5 ml
EUR 227

ExFect2000 Transfection Reagent

T202-02 1 ml
EUR 316

ExFect2000 Transfection Reagent

T202-03 5 ml
EUR 1052

BSA (Reagent Grade)

30-AB79 1 kg
EUR 1552
Description: Reagent Grade Bovine Serum Albumin (99% pure)

BSA (Reagent Grade)

30-AB81 200 grams
EUR 476
Description: Reagent Grade Sulphydryl Blocked BSA (99% pure)

BODIPY-Acetylene Reagent

2594-1
EUR 207

BODIPY-Acetylene Reagent

2594-5
EUR 675

Biotin reagent (HRP)

65C-CE0202 5 mg
EUR 244
Description: HRP conjugated biotin labelling reagent

Bradford Dye Reagent

0209R 100 ml
EUR 131

BCA Reagent, 16ML

C144-16ML 16ML
EUR 163

Dissociation Reagent, 1ML

X017-1ML 1ML
EUR 109

Dissociation Reagent, 25ML

X017-25ML 25ML
EUR 258

Dissociation Reagent, 5ML

X017-5ML 5ML
EUR 122

Dissociation Reagent, 1ML

X058-1ML 1ML
EUR 73

Dissociation Reagent, 5ML

X058-5ML 5ML
EUR 109

Biolipidure-1002-Reagent

Biolipidure-1002-10 10mL
EUR 196
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

LP4K Transfection Reagent

LP4K 1.0 ml / vial
EUR 304
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types.

Tri-RNA Reagent

FATRR-001 100ml
EUR 236

Tri-RNA Reagent

FATRR-002 50ml
EUR 176

Tri-RNA Reagent

FATRR-003 450ml
EUR 645

DTT (Cleland's reagent)

DB0058 5g
EUR 84.8

DTNB (Ellman's Reagent)

DB0113 5g
EUR 97.85

n-Heptane Reagent

HC5400 1L
EUR 79

PhosphoBlocker Blocking Reagent

AKR-104 4L
EUR 711
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

PhosphoBlocker Blocking Reagent

AKR-103 1L
EUR 328
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins.

Ethyl acetate Reagent

EC4600 1L
EUR 79

EL Transfection Reagent

20-abx098880
  • EUR 384.00
  • EUR 537.00
  • 0.75 ml
  • 1.5 ml

Mycoplasma Prevention Reagent

20-abx098886
  • EUR 425.00
  • EUR 509.00
  • 1 ml
  • 5 ml

Convoy? Transfection Reagent

1110-1ml
EUR 341

FcR blocking Reagent

20-abx290024
  • EUR 377.00
  • EUR 516.00
  • 200 tests
  • 400 tests

Detection Reagent A

abx296004-120ul 120 ul
EUR 321

Mycoplasma Prevention Reagent

20-abx298005
  • EUR 203.00
  • EUR 286.00
  • 1 ml
  • 5 ml

Girard's reagent T

20-abx184099
  • EUR 203.00
  • EUR 314.00
  • 100 g
  • 500 g

TissueDigest Reagent, 20X

T101 10ml
EUR 210

HighGene transfection reagent

RM09014 1000μl
EUR 270

Griess Reagent Kit

30100 1KIT
EUR 149
Description: Minimum order quantity: 1 unit of 1KIT

Biolipidure-1002-Reagent

Biolipidure-1002-100 100mL
EUR 1223
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-103-Reagent

Biolipidure-103-10 10mL
EUR 196
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-103-Reagent

Biolipidure-103-100 100mL
EUR 1223
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1201-Reagent

Biolipidure-1201-10 10mL
EUR 196
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1201-Reagent

Biolipidure-1201-100 100mL
EUR 1223
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1301-Reagent

Biolipidure-1301-10 10mL
EUR 196
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-1301-Reagent

Biolipidure-1301-100 100mL
EUR 1223
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-203-Reagent

Biolipidure-203-10 10mL
EUR 196
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-203-Reagent

Biolipidure-203-100 100mL
EUR 1223
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-206-Reagent

Biolipidure-206-10 10mL
EUR 196
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-206-Reagent

Biolipidure-206-100 100mL
EUR 1223
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-405-Reagent

Biolipidure-405-10 10mL
EUR 196
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-405-Reagent

Biolipidure-405-100 100mL
EUR 1223
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-502-Reagent

Biolipidure-502-10 10mL
EUR 196
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-502-Reagent

Biolipidure-502-100 100mL
EUR 1223
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-702-Reagent

Biolipidure-702-10 10mL
EUR 196
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-702-Reagent

Biolipidure-702-100 100mL
EUR 1223
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-802-Reagent

Biolipidure-802-10 10mL
EUR 196
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Biolipidure-802-Reagent

Biolipidure-802-100 100mL
EUR 1223
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Alcohol, Reagent (70%)

EAS500 500 ml
EUR 79

Alcohol, Reagent (70%)

EAS999 1000 ml
EUR 101

PureFection Transfection Reagent

LV750A-1 1 ml
EUR 359

Arabidopsis Thaliana Lysate

30R-AA023 150 ug
EUR 156
Description: Arabidopsis Thaliana Plant Lysate

CMV Cell Lysate

35-1867 1 mg
EUR 1835
Description: CMV enriched cell lysate

HSV1 Cell Lysate

35-1872 1 ml
EUR 394
Description: HSV1 enriched cell lysate

HSV2 Cell Lysate

35-1873 1 ml
EUR 394
Description: HSV2 enriched cell lysate

BL21-lysate Antibody

abx230905-100ug 100 ug
EUR 551

JM109-lysate Antibody

abx234439-100ug 100 ug
EUR 551

DH5a-lysate Antibody

abx232362-100ug 100 ug
EUR 551

Green Algae Lysate

PABL-1306 50 ug
EUR 164

Anti-LITAF (KO validated) Antibody

A2075-100 100 µl
EUR 399

Litaf ORF Vector (Rat) (pORF)

ORF069428 1.0 ug DNA
EUR 506

LITAF ORF Vector (Human) (pORF)

ORF013599 1.0 ug DNA
EUR 354

Litaf ORF Vector (Mouse) (pORF)

ORF049213 1.0 ug DNA
EUR 506

[KO Validated] LITAF Rabbit pAb

A5469-100ul 100 ul
EUR 308

[KO Validated] LITAF Rabbit pAb

A5469-200ul 200 ul
EUR 459

[KO Validated] LITAF Rabbit pAb

A5469-20ul 20 ul
EUR 183

[KO Validated] LITAF Rabbit pAb

A5469-50ul 50 ul
EUR 223

Polyclonal LITAF Antibody (internal region)

APR17202G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human LITAF (internal region). This antibody is tested and proven to work in the following applications:

LITAF ELISA Kit (Chicken) (OKEH04620)

OKEH04620 96 Wells
EUR 844
Description: Description of target: Probable role in regulating transcription of specific genes.;Species reactivity: Chicken;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 34 pg/mL

LITAF ELISA Kit (Rat) (OKEH06185)

OKEH06185 96 Wells
EUR 779
Description: Description of target: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.083 ng/mL

LITAF ELISA Kit (Mouse) (OKEH05545)

OKEH05545 96 Wells
EUR 779
Description: Description of target: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 7.88 pg/mL

LITAF ELISA Kit (Human) (OKEH05546)

OKEH05546 96 Wells
EUR 779
Description: Description of target: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression. ;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 39.4 pg/mL

LITAF ELISA Kit (Mouse) (OKEH06713)

OKEH06713 96 Wells
EUR 779
Description: Description of target: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 15.6 pg/mL

LITAF ELISA Kit (Rat) (OKEH06714)

OKEH06714 96 Wells
EUR 779
Description: Description of target: Probable role in regulating transcription of specific genes. May regulate through NFKB1 the expression of the CCL2/MCP-1 chemokine. May play a role in tumor necrosis factor alpha (TNF-alpha) gene expression;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.156 ng/mL

Chicken IgY precipitation reagent

85R-1010 4 ml
EUR 554
Description: Chicken IgY precipitation reagent for use in immunoassays

Mouse IgG Blocking Reagent

30R-AH071 20 mg
EUR 258
Description: Mouse IgG (H + L) Blocking Reagent

Biotin reagent (Alk Phos)

65C-CE0201 1 mg
EUR 211
Description: Alkaline Phosphatase conjugated biotin labelling reagent

Biotin reagent (Glucose Oxidase)

65C-CE0203 5 mg
EUR 232
Description: Glucose Oxidase conjugated biotin labelling reagent

Annexin V-Cy5 Reagent

1013-1000
EUR 1132

Annexin V-Cy5 Reagent

1013-200
EUR 403

Annexin V-PE Reagent

1014-1000
EUR 1132

Annexin V-PE Reagent

1014-200
EUR 403

Annexin V-FITC Reagent

1001-1000
EUR 1246

Annexin V-FITC Reagent

1001-200
EUR 441

Annexin V-Cy3 Reagent

1002-1000
EUR 1132

Annexin V-Cy3 Reagent

1002-200
EUR 403

Annexin V-Biotin Reagent

1003-100
EUR 370

Annexin V-Biotin Reagent

1003-1000
EUR 2388

Annexin V-EGFP Reagent

1004-1000
EUR 1132

Annexin V-EGFP Reagent

1004-200
EUR 403

Cytotoxicity Control Reagent (Saponin)

CTTX-050 (50mg)
EUR 135
Description: To be used as a cytotoxic control agent in the CellQuanti-BlueTM and CellQuanti-MTTTM Cell Viability Assay Kits. IC50 = 0.002 wt%. Kit size: (50mg) tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Creatinine Color Reagent, 28ML

C004-28ML 28ML
EUR 268

GST Detection Reagent, 1EA

C035-1EA 1EA
EUR 237

Hemoglobin Detection Reagent, 20ML

C038-20ML 20ML
EUR 258

Hydrogen Peroxide Reagent, 5ML

C115-5ML 5ML
EUR 123

Fluorescent Detection Reagent, 5ML

C116-5ML 5ML
EUR 268

Hydrogen Peroxide Reagent, 5ML

C132-5ML 5 ML
EUR 123

FRAP Reagent A, 1.4ML

C148-1.4ML 1.4ML
EUR 178

FRAP Reagent B, 1.4ML

C149-1.4ML 1.4ML
EUR 178

Detection Reagent Concentrate, 1ML

X041-1ML 1ML
EUR 143
Arbitrary or unconsidered transformation can result in flawed conclusions for the MVA cluster strategies, dimensionality discount strategies and classification strategies. We advocate transformation of circulation cytometry knowledge through the use of OTflow-defined parameters estimated per channel, so as to forestall peak-splitting and different artifacts within the knowledge. This text is protected by copyright. All rights reserved.

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