Isolation and immunophenotyping by flow cytometry of canine peripheral blood and intraepithelial and lamina propria duodenal T lymphocytes

Isolation and immunophenotyping by flow cytometry of canine peripheral blood and intraepithelial and lamina propria duodenal T lymphocytes

The intestine related lymphoid tissue (GALT) effector websites play a vital position on the pathogenesis of many immune-mediated gastrointestinal ailments. The lymphocytes at these effector websites are principally T cells which current necessary morphological, phenotypical and purposeful variations. Circulation cytometry (FC) is likely one of the mostly used methods to characterize intestinal lymphocytes in human and animal fashions. Printed research with a deal with canine for intraepithelial lymphocytes (IEL) immunophenotyping exist in very restricted numbers.

Furthermore, no lamina propria lymphocytes (LPL) isolation protocols within the canine species have been described for FC analysis. Along with immune intestinal dysregulation, imbalances within the peripheral blood immune system have been described in each human and animal gastrointestinal issues. The goal of this examine was to supply a protocol for canine IEL and LPL isolation for FC immunophenotyping of T cells subsets. Particularly, T helper, T cytotoxic, activated Th and Tc lymphocytes, regulatory, double unfavorable, double optimistic, IFN-γ and IL-Four producing T cells, and to match their respective populations between these effector websites and with the blood stream compartment in wholesome canine.

The potential relationship of those cells distributions with age, intercourse and breed was additionally evaluated. This examine included sixteen wholesome canine of various sexes and breeds with a imply age of 4.55 ± 2.93 years previous. The chosen protocols for the three immune compartments confirmed correct cell yield, purity, viability, and the absence of phenotypic and purposeful disturbances. Histologically, an satisfactory separation of the duodenal epithelium from the lamina propria was additionally noticed.

All of the proposed T cells subsets have been recognized within the three immune compartments studied, exhibiting some statistically vital variations of their distributions at these areas that spotlight the significance of their particular person analysis. This examine offers an satisfactory methodology for canine small gut IEL and LPL isolation for FC immunophenotyping and is vital for future research on the gastrointestinal immune system related to completely different canine ailments.

Circulation Cytometry Detection of Sperm DNA Fragmentation and Apoptotic Markers within the Semen of Infertile Males

The impact of sperm molecular defects on fertilization and being pregnant end result after assisted reproductive remedy (ART) is extensively documented by each analysis and medical societies. Sperm DNA fragmentation and irregular chromatin condensation symbolize essential causes of male infertility. Superior androgenic methods for precisely figuring out molecular defects assist in deciding on an applicable therapy technique. Moreover, particular markers of apoptosis are more and more necessary in predicting male infertility. The power of circulation cytometry to estimate the amount of sperm with DNA fragmentation or injury and multifactor measurements in immotile sperm have made this developed approach important in fertility facilities.

Isolation and immunophenotyping by flow cytometry of canine peripheral blood and intraepithelial and lamina propria duodenal T lymphocytes

The examine is geared toward assessing the extent of DNA fragmentation and apoptosis by measuring circulation cytometry utilizing new methods. Circulation cytometry evaluation revealed a various diploma of DNA injury. It was in a position to quantify the diploma of impairment even in samples with minimal DNA fragmentation. DNA injury was noticed even in samples that have been thought of regular with a routine semen evaluation. Circulation cytometry was delicate to adjustments in sperm apoptosis. Elevated p53 exercise ranges have been related to excessive DNA fragmentation. In the meantime, B-cell lymphoma 2 (Bcl-2) actions confirmed a unique sample. In conclusion, circulation cytometry for sperm DNA fragmentation and markers of apoptosis could be a priceless instrument in assisted reproductive facilities.

Transformation of Multicolour Circulation Cytometry knowledge with OTflow prevents deceptive multivariate evaluation (MVA) outcomes and incorrect immunological conclusions

The speedy evolution of the circulation cytometry subject, at present permitting the measurement of 30-50 parameters per cell, has led to a marked enhance in deep multivariate info. Handbook gating is inadequate to extract all this info. Due to this fact, multivariate evaluation (MVA) strategies have been developed to extract info and effectively analyse the high-density multicolour circulation cytometry (MFC) knowledge. To help interpretation, MFC knowledge are sometimes logarithmically reworked earlier than MVA.
We studied the implications of various transformations of circulation cytometry knowledge in datasets containing unfavorable intensities attributable to background subtractions and spreading error, as logarithmic transformation of unfavorable knowledge is inconceivable. Transformations akin to logicle or hyperbolic arcsine transformations permit linearity round zero, whereas larger (optimistic and unfavorable) intensities are logarithmically reworked. To outline the linear vary, a parameter (or cofactor) have to be chosen.
We present how the chosen transformation parameter has nice impression on the MVA outcomes. In some instances, peak-splitting is noticed, producing two distributions round zero in an precise homogeneous inhabitants. This can be misinterpreted because the presence of a number of cell populations. Furthermore, when performing arbitrary transformation earlier than MVA evaluation, biologically related and statistically vital info may be missed. We current a brand new algorithm, Optimum Transformation for circulation cytometry knowledge (OTflow), which makes use of numerous statistical strategies to optimally select the parameter of the transformation and forestall artifacts akin to peak splitting.

baculoCOMPLETE protein expression kit + baculoQUANT all-in-one

400101 5 + 100 reactions
EUR 1419.8

Human LITAF Protein Lysate

MBS8414542-002mg 0.02mg
EUR 365

Human LITAF Protein Lysate

MBS8414542-5x002mg 5x0.02mg
EUR 1410

Human LITAF Protein Lysate 20ug

IHULITAFPLLY20UG each
EUR 213
Description: Human LITAF Protein Lysate 20ug

OCOA19001-20UG - LITAF Protein Lysate

OCOA19001-20UG 20ug
EUR 169

OCOA19002-20UG - LITAF Protein Lysate

OCOA19002-20UG 20ug
EUR 169

OCOA12906-20UG - LITAF Protein Lysate

OCOA12906-20UG 20ug
EUR 169

Protein expression

CRO-4-Ind each Ask for price

EP Reagent Biuret Reagent - 100ML

1011601 1L
EUR 59.4

RFP expression Adenovirus, in vivo ready

AVP001-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made RFP expression adenovirus, provided in PBS solution.

CFP expression Adenovirus, in vivo ready

AVP002-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made CFP expression adenovirus, provided in PBS solution.

GFP expression Adenovirus, in vivo ready

AVP011-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made GFP expression adenovirus, provided in PBS solution.

YFP expression Adenovirus, in vivo ready

AVP012-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made YFP expression adenovirus, provided in PBS solution.

BFP expression Adenovirus, in vivo ready

AVP017-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made BFP expression adenovirus, provided in PBS solution.

PEROXIDE BLOCKING REAGENT Reagent

GWB-Q00308 50 ml Ask for price

YLEX Expression Kit

FYY201-1KT 1 kit Ask for price

EP Reagent Iodoplatinate Reagent - 200ML

1046300 200ML
EUR 502.2

EP Reagent Molybdovanadic Reagent - 100ML

1056700 100ML
EUR 59.4

h OCT4 expression Adenovirus, in vivo ready

AVP013-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application.

h SOX2 expression Adenovirus, in vivo ready

AVP014-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application.

293 Expression MAX-1

EXP-711-11 1ml
EUR 214
Description: 293 Expression MAX-1 is expressed from - -.

EP Reagent Methoxyphenylacetic Reagent - 100ML

1053601 100ML
EUR 490.05

h LIN28 expression Adenovirus, in vivo ready

AVP015-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application.

CNGA2 Expression vector

60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

CNGA2 Expression vector

GWB-PSFA61 20ug Ask for price

EP Reagent Phosphomolybdotungstic Reagent - 100ML

1065000 100ML
EUR 226.8

LEXSYcon2.1 Expression Kit

EGE-1310ble 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310bsd 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310hyg 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310neo 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310pac 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

LEXSYcon2.1 Expression Kit

EGE-1310sat 1Kit
EUR 1296.1
Description: for constitutive cytosolic or secretory protein expression

pYLEX1 - Expression Vector

FYY203-5MG 5mg Ask for price

Blunt E1 Expression Kit

abx098098-10rxns 10 rxns
EUR 560.4

Blunt E2 Expression Kit

abx098873-10rxns 10 rxns
EUR 560.4

Blunt E1 Expression Kit

abx098098-100l 100 µl
EUR 712.5

Blunt E1 Expression Kit

abx098098-1ml 1 ml Ask for price

Blunt E1 Expression Kit

abx098098-200l 200 µl Ask for price

Blunt E2 Expression Kit

abx098873-100l 100 µl
EUR 712.5

Blunt E2 Expression Kit

abx098873-1ml 1 ml Ask for price

Blunt E2 Expression Kit

abx098873-200l 200 µl Ask for price

AVIDIN/BIOTIN BLOCKING REAGENT Reagent

GWB-Q00289 30 ml Ask for price

RFP expression Adenovirus

AVP001 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus

AVP002 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus

AVP011 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus

AVP012 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made YFP expression adenovirus, provided in DMEM medium.

BFP expression Adenovirus

AVP017 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made BFP expression adenovirus, provided in DMEM medium.

Gordon-McLeod Reagent (Oxidase reagent)

R026-100ML 1 unit
EUR 7.76
Description: Gordon-McLeod Reagent (Oxidase reagent)

Oxidase Reagent (Gordon-Macleod Reagent)

76207 100 ml
EUR 3.38
Description: Part B

LEXSinduce3 Expression Kit

EGE-1410ble 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce3 Expression Kit

EGE-1410blecherry 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce3 Expression Kit

EGE-1410neo 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

LEXSinduce4 Expression Kit

EGE-1420blecherry 1Kit
EUR 3037.6
Description: for inducible cytosolic or secretory protein expression

Rac1 Expression Vector Set

STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set

STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

NEU3 Over-expression Lysate

GWB-DL2468 0.1 mg Ask for price

EP Reagent Sulfomolybdic Reagent R3 - 1L

1086500 1L
EUR 398.25

TRIpure Reagent (same as Trizol Reagent)

MBS356001-INQUIRE INQUIRE Ask for price

Cdc42 Expression Vector Set

STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

OPN expression inhibitor 1

T62820-10mg 10mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-1g 1g Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-1mg 1mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-50mg 50mg Ask for price
Description: OPN expression inhibitor 1

OPN expression inhibitor 1

T62820-5mg 5mg Ask for price
Description: OPN expression inhibitor 1

Total RNA Isolation Reagent (Reagent Based)

MBS402052-100mL 100mL
EUR 235

Total RNA Isolation Reagent (Reagent Based)

MBS402052-4Reactions 4Reactions
EUR 165

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 530

h IL6 Expression Lentivirus

LVP1144 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD4 Expression Lentivirus

LVP1152 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.

h IL4 Expression Lentivirus

LVP1161 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.

h IL7 Expression Lentivirus

LVP1162 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KDR Expression Lentivirus

LVP1176 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.

h FAS Expression Lentivirus

LVP1181 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker.

h ADA Expression Lentivirus

LVP923 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.

h FAP Expression Lentivirus

LVP1307 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FAP, containing a RFP-Blasticidin dual selection marker.

h CD1 Expression Lentivirus

LVP1320 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CD1, containing a RFP-Blasticidin dual selection marker.

m FAP Expression Lentivirus

LVP1326 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: FAP, containing a RFP-Blasticidin dual selection marker.

h VIM Expression Lentivirus

LVP1357 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: VIM, containing a RFP-Blasticidin dual selection marker.

h PXN Expression Lentivirus

LVP1358 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PXN, containing a RFP-Blasticidin dual selection marker.

m CD8 Expression Lentivirus

LVP1363 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: CD8, containing a RFP-Blasticidin dual selection marker.

h TPO Expression Lentivirus

LVP1371 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: TPO, containing a RFP-Blasticidin dual selection marker.

h WAS Expression Lentivirus

LVP1429 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: WAS, containing a RFP-Blasticidin dual selection marker.

m PGR Expression Lentivirus

LVP1455 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for mouse target: PGR containing a RFP-Blasticidin dual selection marker.

h MAG Expression Lentivirus

LVP1485 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: MAG, containing a RFP-Blasticidin dual selection marker.

h FST Expression Lentivirus

LVP1518 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human FST gene, containing a RFP-Blasticidin dual selection marker.

pAAV-MCS Expression Vector

MBS169528-001mg 0.01mg
EUR 695

pAAV-MCS Expression Vector

MBS169528-5x001mg 5x0.01mg
EUR 2955

FlashBAC - Baculovirus Expression System (96 reactions)

GWB-6C34E3 Kit Ask for price

FlashBAC - Baculovirus Expression System (5 reactions)

GWB-7676BB Kit Ask for price

FlashBAC - Baculovirus Expression System(24 reactions)

GWB-EB1FF8 Kit Ask for price

Genomic DNA Isolation Reagent (Reagent Based)

MBS402050-100mL 100mL
EUR 255

Genomic DNA Isolation Reagent (Reagent Based)

MBS402050-4Reactions 4Reactions
EUR 185

h OCT4 expression Adenovirus

AVP013 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium.

h SOX2 expression Adenovirus

AVP014 1x109 IFU/ml x 50ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium.

h EGFR Expression Lentivirus

LVP1142 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker.

h NRP1 Expression Lentivirus

LVP1145 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h MUC1 Expression Lentivirus

LVP1148 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD8a Expression Lentivirus

LVP1151 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker.

h PIGF Expression Lentivirus

LVP1154 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker.

h BRAF Expression Lentivirus

LVP1156 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker.

h ROS1 Expression Lentivirus

LVP1158 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker.

h CD3E Expression Lentivirus

LVP1163 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KLK3 Expression Lentivirus

LVP1171 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker.

h NEK6 Expression Lentivirus

LVP1182 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker.

h NEK7 Expression Lentivirus

LVP1183 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker.

h CDR1 Expression Lentivirus

LVP917 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker.

h CD47 Expression Lentivirus

LVP918 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker.

h ESR1 Expression Lentivirus

LVP924 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker.

r Syn1 Expression Lentivirus

LVP925 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker.

h NME4 Expression Lentivirus

LVP932 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker.

h STM2 Expression Lentivirus

LVP1210 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Pre-made over-expression lentivirus for human target: STM2, containing a RFP-Blasticidin dual selection marker.

h CBX7 Expression Lentivirus

LVP1308 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CBX7, containing a RFP-Blasticidin dual selection marker.

h ACE2 Expression Lentivirus

LVP1310 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ACE2, containing a RFP-Blasticidin dual selection marker.

h Rab7 Expression Lentivirus

LVP1315 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Rab7, containing a RFP-Blasticidin dual selection marker.

h Rab5 Expression Lentivirus

LVP1316 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Rab5, containing a RFP-Blasticidin dual selection marker.

m ASPA Expression Lentivirus

LVP1317 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for mouse target: ASPA, containing a RFP-Blasticidin dual selection marker.

h CD44 Expression Lentivirus

LVP1318 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CD44, containing a RFP-Blasticidin dual selection marker.

h MFN2 Expression Lentivirus

LVP1327 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: MFN2, containing a RFP-Blasticidin dual selection marker.

h ANO1 Expression Lentivirus

LVP1344 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ANO1 containing a RFP-Blasticidin dual selection marker.

h FGL2 Expression Lentivirus

LVP1346 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FGL2, containing a RFP-Blasticidin dual selection marker.

h CCR2 Expression Lentivirus

LVP1354 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CCR2, containing a RFP-Blasticidin dual selection marker.

h NEFL Expression Lentivirus

LVP1360 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NEFL, containing a RFP-Blasticidin dual selection marker.

h NOS2 Expression Lentivirus

LVP1367 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NOS2, containing a RFP-Blasticidin dual selection marker.

h BMI1 Expression Lentivirus

LVP1372 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: BMI1, containing a RFP-Blasticidin dual selection marker.

h PSCA Expression Lentivirus

LVP1374 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PSCA, containing a RFP-Blasticidin dual selection marker.

h POLQ Expression Lentivirus

LVP1421 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: POLQ containing a RFP-Blasticidin dual selection marker.

h ARSA Expression Lentivirus

LVP1425 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: ARSA, containing a RFP-Blasticidin dual selection marker.

h IDUA Expression Lentivirus

LVP1426 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: IDUA, containing a RFP-Blasticidin dual selection marker.

h SGSH Expression Lentivirus

LVP1427 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: SGSH, containing a RFP-Blasticidin dual selection marker.

h CYBB Expression Lentivirus

LVP1430 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CYBB, containing a RFP-Blasticidin dual selection marker.

h CAV1 Expression Lentivirus

LVP1431 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CAV1, containing a RFP-Blasticidin dual selection marker.

m INS2 Expression Lentivirus

LVP1456 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for mouse target: INS2 containing a RFP-Blasticidin dual selection marker.

h ROR1 Expression Lentivirus

LVP1458 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: ROR1, containing a RFP-Blasticidin dual selection marker.

h DGKA Expression Lentivirus

LVP1468 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: DGKA, containing a RFP-Blasticidin dual selection marker.

h REST_HA Expression Lentivirus

LVP1475 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: REST, containing a RFP-Blasticidin dual selection marker.

h UBR7 Expression Lentivirus

LVP1483 1x10^7 IFU/ml x 200ul
EUR 346.5
Description: Pre-made over-expression lentivirus for human target: UBR7, containing a RFP-Blasticidin dual selection marker.

h MMP1 Expression Lentivirus

LVP1516 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human MMP1 gene, containing a RFP-Blasticidin dual selection marker.

h BDNF Expression Lentivirus

LVP1520 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express human BDNF gene, containing a RFP-Blasticidin dual selection marker.

m CCL5 Expression Lentivirus

LVP1619 1x10^8 IFU/ml x 200ul
EUR 455
Description: Pre-made lentivirus express mouse CCL5 gene, containing a RFP-Blasticidin dual selection marker.

h LIN28 expression Adenovirus

AVP015 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium.

pscAAV-MCS Expression Vector

VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

h HDAC1 Expression Lentivirus

LVP1143 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h VEGFB Expression Lentivirus

LVP1146 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h PARP1 Expression Lentivirus

LVP1149 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h IL3RA Expression Lentivirus

LVP1150 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker.

h PTCH1 Expression Lentivirus

LVP1155 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker.

h SART3 Expression Lentivirus

LVP1159 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker.

h TIGIT Expression Lentivirus

LVP1160 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker.

h EDNRA Expression Lentivirus

LVP1165 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker.

h Foxp3 Expression Lentivirus

LVP1172 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker.

m RIKEN Expression Lentivirus

LVP1174 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker.

h APLP2 Expression Lentivirus

LVP1177 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker.

h PCSK9 Expression Lentivirus

LVP1178 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker.

h NTSR1 Expression Lentivirus

LVP1179 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker.

h GPNMB Expression Lentivirus

LVP921 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: GPNMB, containing a RFP-Blasticidin dual selection marker.

h DECR1 Expression Lentivirus

LVP927 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR1, containing a RFP-Blasticidin dual selection marker.

h DECR2 Expression Lentivirus

LVP928 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR2, containing a RFP-Blasticidin dual selection marker.

h NMRK1 Expression Lentivirus

LVP930 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NMRK1, containing a RFP-Blasticidin dual selection marker.

h PITX3 Expression Lentivirus

LVP931 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PITX3, containing a RFP-Blasticidin dual selection marker.

h FADS2 Expression Lentivirus

LVP1319 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: FADS2, containing a RFP-Blasticidin dual selection marker.

h OVOL2 Expression Lentivirus

LVP1322 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: OVOL2, containing a RFP-Blasticidin dual selection marker.

h APOA1 Expression Lentivirus

LVP1324 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: APOA1, containing a RFP-Blasticidin dual selection marker.

h SOX10 Expression Lentivirus

LVP1325 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: SOX10, containing a RFP-Blasticidin dual selection marker.

h P2RY1 Expression Lentivirus

LVP1328 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: P2RY1, containing a RFP-Blasticidin dual selection marker.

h TUSC2 Expression Lentivirus

LVP1343 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: TUSC2, containing a RFP-Blasticidin dual selection marker.

h NLRP3 Expression Lentivirus

LVP1350 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: NLRP3, containing a RFP-Blasticidin dual selection marker.

h CCL19 Expression Lentivirus

LVP1353 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: CCL19, containing a RFP-Blasticidin dual selection marker.

h Sirt2 Expression Lentivirus

LVP1373 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: Sirt2, containing a RFP-Blasticidin dual selection marker.

h EPCAM Expression Lentivirus

LVP1375 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: EPCAM, containing a RFP-Blasticidin dual selection marker.

h PDE6B Expression Lentivirus

LVP1423 1x10^7 IFU/ml x 200ul
EUR 276.5
Description: Over-expression lentivirus for human target: PDE6B, containing a RFP-Blasticidin dual selection marker.
Arbitrary or unconsidered transformation can result in flawed conclusions for the MVA cluster strategies, dimensionality discount strategies and classification strategies. We advocate transformation of circulation cytometry knowledge through the use of OTflow-defined parameters estimated per channel, so as to forestall peak-splitting and different artifacts within the knowledge. This text is protected by copyright. All rights reserved.

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