Human IgM ELISA Kit

Product name

Human IgM, ELISA kit

Full product name

Human IgM ELISA Kit

For research use only

For research use only. It should not be used in diagnostic procedures.

Species reactivity: Human

Samples

Immunoperoxidase assay for the determination of IgM in human samples

Test type: sandwich

Intended uses

The Total Human IgM Test Kit is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for measuring IgM in human biological samples.

Preparation and Storage

Store at 4 degrees C.

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, sometimes resulting in the ambiguous or non-relevant product information. It is the customer’s responsibility to review, verify and evaluate the information to ensure it matches their requirements prior to purchasing the kit. Our ELISA Kit assays are dynamic research tools and may be updated and improved from time to time.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current trial details. We cannot guarantee that the sample manual posted online is the most up-to-date manual, it is intended to serve as an example only. Consult the instructions for use provided with the assay kit for precise details.

Other notes

On occasion, small volumes of human IgM Elisa kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly spin the vial in a tabletop centrifuge to dislodge any liquid in the vial cap. Certain products may require dry ice shipping and an additional dry ice fee may apply.

Search terms for the purchase of human IgM

MBS564051 is a ready-to-use microwell, strip, or full plate ELISA (Enzyme-Linked Immunosorbent Assay) kit for testing the presence of Human IgM ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls generate a theoretical detection range of the kit in biological research samples containing human IgM.

The MBS564051 ELISA analytical biochemical technique is based on human IgM antibody-human IgM antigen interactions (immunosorbent) and an HRP colourimetric detection system to detect human IgM antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant human IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, and secretions. Quality control assays that evaluated reproducibility identified intra-assay CV (%) and inter-assay CV (%).

Product related information for human IgM Elisa kit

Assay Principle: The principle of the double-antibody sandwich ELISA is depicted in Figure 1. In this assay, IgM present in samples reacts with anti-IgM antibodies that have been adsorbed on the surface of polystyrene microtiter wells. . After removing unbound proteins by washing, horseradish peroxidase (HRP)-conjugated anti-IgM antibodies are added.

These enzyme-labelled antibodies form complexes with previously bound IgM. After another wash step, the immunosorbent-bound enzyme is analyzed by the addition of a chromogenic substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). The amount of bound enzyme varies directly with the concentration of IgM in the tested sample; therefore, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The amount of IgM in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

Precautions

All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe the intended use will not meet our acceptable guidelines.

Disclaimer

While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource shall not be responsible for any omissions or errors contained in this document. MyBioSource reserves the right to make changes to this datasheet at any time without notice.

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