Grade: reagent grade
Test: ≥95% (HPLC)
Way: buffered aqueous solution
Storage temperature: −20°C
Purified human IgA Elisa Kit can be used to study its physiological and biological effects on processes such as inflammation, pathogen clearance, regulation of antibody-dependent cell-mediated cytotoxicity (ADCC), and modulation of phagocytosis, eosinophil degranulation and basophils and the modulation of the respiratory burst exercise.
The solution in 0.01 M phosphate-buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
Principle of the method
The Human IgA Solid Phase Sandwich ELISA (Enzyme-Linked Immunosorbent Assay) is designed to measure the amount of bound target between a pair of matched antibodies. A target-specific antibody has been pre-coated on the wells of the supplied microplate. Samples, standards, or controls are then added to these wells and bound to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added which reacts with the enzyme-antibody-target complex to produce a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original sample.
The isotype of a primary antibody and the application in which it is used can cause background staining. Background noise from the primary antibody may be due to binding to Fc receptors on target cells; nonspecific interactions with cellular proteins, carbohydrates, and lipids; or cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signals from specific antibody signals because they do not have relevant specificity for a target antigen.
Although isotype controls are most often used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls must be matched to the primary antibody species and isotype so that the level of primary antibody-specific staining can be accurately determined. If using directly labelled primary antibodies, the Isotype Control works best if it is conjugated to the same label as the test antibody.