Properties

Analytical sensitivity: 0.24ng/mL

Test range: 1.6-100ng/mL

Sample type/volume

• Plasma: 10μL
• Serum: 10μL

Hands-on time: 1 hour 20 minutes

Time to result: 1 hour 30 minutes

Homogeneous (without washing): Do not

Inter-assay CV: 2.6%

Intra-assay CV: 5.3%

Instrument: Colorimetric microplate reader

Product size: 96 tests

Content

• Pre-coated 96-well plate
• Standard
• Sample diluent
• Assay buffer concentrate
• HRP-conjugated detection antibody
• wash buffer
• chromogen
• stop solution
• Control S
• Adhesive plate covers

Storage: 2-8°C

About this team

The Human Total Immunoglobulin G (Hu IgG) ELISA Kit quantifies Hu IgG in human serum or plasma. The assay will exclusively recognize natural and recombinant Hu IgG.

Principle of the method

The Human IgG Solid Phase Sandwich ELISA (Enzyme-Linked Immunosorbent Assay) is designed to measure the amount of bound target between a pair of matched antibodies. A target-specific antibody has been pre-coated on the wells of the supplied microplate. Samples, standards, or controls are then added to these wells and bound to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added which reacts with the enzyme-antibody-target complex to produce a measurable signal. The intensity of this signal is directly proportional to the concentration of the target present in the original sample.

Destination information

The isotype of a primary antibody and the application in which it is used can cause background staining. Background noise from the primary antibody may be due to binding to Fc receptors on target cells; nonspecific interactions with cellular proteins, carbohydrates, and lipids; or cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signals from specific antibody signals because they do not have relevant specificity for a target antigen.

Although isotype controls are most often used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls must match the species and isotype of the primary antibody so that the level of specific staining of the primary antibody can be accurately determined. If using directly labelled primary antibodies, the Isotype Control works best if it is conjugated to the same label as the test antibody.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested against criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See the manual for more information on validation.

For research use only. It should not be used in diagnostic procedures. Not for resale without express permission.