Highly sensitive detection of antibody nonspecific interactions using flow cytometry

The quickly evolving nature of antibody drug improvement has resulted in applied sciences that generate huge numbers (a whole bunch to hundreds) of lead antibody candidates throughout early discovery. These candidates have to be quickly pared all the way down to establish probably the most drug-like candidates for in-depth evaluation of their security and efficacy, which may solely be carried out on a restricted variety of antibodies attributable to time and useful resource necessities. One key biophysical property of profitable antibody therapeutics is excessive specificity, outlined as low ranges of nonspecific binding or polyspecificity.

Though there was some progress in creating assays for detecting antibody polyspecificity, most of those assays are restricted by poor sensitivity or assay codecs that require proprietary antibody floor show strategies, and a few of these assays use advanced and poorly outlined polyspecificity reagents. Right here we report the PolySpecificity Particle (PSP) assay, a delicate circulation cytometry assay for evaluating antibody nonspecific interactions that overcomes earlier limitations and can be utilized for evaluating various sorts of IgGs, multispecific antibodies and Fc-fusion proteins. Our strategy makes use of micron-sized magnetic beads coated with Protein A to seize antibodies at extraordinarily dilute concentrations (<0.02 mg/mL).

Move cytometry evaluation of polyspecificity reagent binding to those conjugates ends in delicate detection of variations in nonspecific interactions for clinical-stage antibodies. Our PSP assay strongly discriminates between antibodies with totally different ranges of polyspecificity utilizing beforehand reported polyspecificity reagents which are both well-defined proteins or extremely advanced protein mixtures. Furthermore, we additionally discover {that a} distinctive reagent, particularly ovalbumin, ends in the very best assay sensitivity and specificity. Importantly, our assay is far more delicate than customary assays equivalent to ELISAs. We anticipate that our easy, delicate, and high-throughput PSP assay will speed up the event of protected and efficient antibody therapeutics.

Toxicity of Carbon Nanomaterials-In the direction of Dependable Viability Evaluation by way of New Method in Move Cytometry

The scope of software of carbon nanomaterials in biomedical, environmental and industrial fields is lately considerably growing. Since in vitro toxicity testing is the primary important step for any business utilization, it’s essential to have a dependable methodology to investigate the possibly dangerous results of carbon nanomaterials. Regardless that researchers already reported the interference of carbon nanomaterials with widespread toxicity assays, there’s nonetheless, sadly, a lot of research that neglect this reality. On this research, we investigated interference of 4 bio-promising carbon nanomaterials (graphene acid (GA), cyanographene (GCN), graphitic carbon nitride (g-C3N4) and carbon dots (QCDs)) in generally used LIVE/DEAD assay.

When a regular process was utilized, supplies prompted varied sorts of interference. Whereas positively charged g-C3N4 and QCDs induced false outcomes by means of the creation of free agglomerates and intrinsic fluorescence properties, negatively charged GA and GCN led to false indicators as a result of advanced quenching impact of the fluorescent dye of a LIVE/DEAD package. Thus, we developed a brand new strategy utilizing a particular gating technique primarily based on extra controls that efficiently overcame all sorts of interference and result in dependable ends in LIVE/DEAD assay.

We advise that the newly developed process ought to be a compulsory device for all in vitro circulation cytometry assays of any class of carbon nanomaterials. Imaging circulation cytometry permits for the quantitative evaluation of fluorescent indicators on the subcellular degree. Right here, we describe using a biosensor cell line, particularly, U2OS osteosarcoma cells outfitted with a fusion protein comprising monomeric pink fluorescent protein (mRFP), inexperienced fluorescent protein (GFP) and microtubule-associated proteins 1A/1B gentle chain 3B (greatest often called LC3), for the evaluation of autophagic flux by imaging circulation cytometry.

Highly sensitive detection of antibody nonspecific interactions using flow cytometry

We element all evaluation instruments required to tell apart autophagosomes (that emit each a pink and a inexperienced fluorescence) and autolysosomes (that emit a pink fluorescence, but lose the inexperienced fluorescent sign) and to quantitate autophagic flux in a handy style. Our information, nevertheless, present that information of the kinetics of the interplay permits to reconcile the info obtained by circulation cytometry and LigandTracer and show the complementarity of those two strategies.

Okay D willpower from time-resolved experiments on stay cells with LigandTracer and reconciliation with end-point circulation cytometry measurements

Design of next-generation therapeutics comes with new challenges and emulates expertise and strategies to satisfy them. Characterizing the binding of both pure ligands or therapeutic proteins to cell-surface receptors, for which related recombinant variations could not exist, represents certainly one of these challenges. Right here we report the characterization of the interplay of 5 totally different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate goal receptors utilizing LigandTracer.

Rabbit Polyclonal antibody Anti-CRBN

Anti-CRBN 50 µg
EUR 418.8

Anti-MCCC2 (2B3)

YF-MA19192 100 ug
EUR 435.6
Description: Mouse monoclonal to MCCC2

MCCC2 antibody

70R-18436 50 ul
EUR 522
Description: Rabbit polyclonal MCCC2 antibody

MCCC2 Antibody

1-CSB-PA867199ESR1HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against MCCC2. Recognizes MCCC2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

MCCC2 Antibody

DF12655 200ul
EUR 420

MCCC2 Antibody

1-CSB-PA013573GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against MCCC2. Recognizes MCCC2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

MCCC2 Polyclonal Antibody

31405-100ul 100ul
EUR 302.4

MCCC2 Polyclonal Antibody

31405-50ul 50ul
EUR 224.4

MCCC2 Polyclonal Antibody

28858-100ul 100ul
EUR 302.4

MCCC2 Polyclonal Antibody

28858-50ul 50ul
EUR 224.4

Mccc2/ Rat Mccc2 ELISA Kit

ELI-48233r 96 Tests
EUR 1063.2

MCCC2 siRNA

20-abx903187
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

MCCC2 siRNA

20-abx923707
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

MCCC2 siRNA

20-abx923708
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

Polyclonal MCCC2 Antibody (Center)

APR10893G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MCCC2 (Center). This antibody is tested and proven to work in the following applications:

MCCC2 Polyclonal Conjugated Antibody

C28858 100ul
EUR 476.4

MCCC2 Polyclonal Conjugated Antibody

C31405 100ul
EUR 476.4

Polyclonal Goat anti-GST α-form

GST-ANTI-1 50 uL
EUR 336

Polyclonal Goat anti-GST μ-form

GST-ANTI-2 50 uL
EUR 336

Polyclonal Goat anti-GST p-form

GST-ANTI-3 50 uL
EUR 336

MCCC2 Rabbit pAb

A15181-100ul 100 ul
EUR 369.6

MCCC2 Rabbit pAb

A15181-200ul 200 ul
EUR 550.8

MCCC2 Rabbit pAb

A15181-20ul 20 ul
EUR 219.6

MCCC2 Rabbit pAb

A15181-50ul 50 ul
EUR 267.6

MCCC2 Rabbit pAb

A7990-100ul 100 ul
EUR 369.6

MCCC2 Rabbit pAb

A7990-200ul 200 ul
EUR 550.8

MCCC2 Rabbit pAb

A7990-20ul 20 ul
EUR 219.6

MCCC2 Rabbit pAb

A7990-50ul 50 ul
EUR 267.6

MCCC2 cloning plasmid

CSB-CL867199HU1-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the MCCC2 gene.

MCCC2 cloning plasmid

CSB-CL867199HU2-10ug 10ug
EUR 451.2
Description: A cloning plasmid for the MCCC2 gene.

MCCC2 Blocking Peptide

DF12655-BP 1mg
EUR 234

[One Step] MCCC2 Antibody Kit

RK05665 50 ul
EUR 288

Mouse MCCC2 shRNA Plasmid

20-abx978856
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Human MCCC2 shRNA Plasmid

20-abx961908
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Rat MCCC2 shRNA Plasmid

20-abx990287
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

MCCC2 ELISA KIT|Human

EF000692 96 Tests
EUR 826.8

Mouse Mccc2 ELISA KIT

ELI-46423m 96 Tests
EUR 1038

Human MCCC2 ELISA KIT

ELI-15862h 96 Tests
EUR 988.8

MCCC2 Recombinant Protein (Human)

RP018958 100 ug Ask for price

MCCC2 Recombinant Protein (Human)

RP018961 100 ug Ask for price

MCCC2 Recombinant Protein (Rat)

RP211097 100 ug Ask for price

MCCC2 Recombinant Protein (Mouse)

RP149789 100 ug Ask for price

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

20-abx006595
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx033068-400ul 400 ul
EUR 627.6

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx033068-80l 80 µl
EUR 343.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx030686-400ul 400 ul
EUR 627.6

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx030686-80l 80 µl
EUR 343.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx037012-100ug 100 ug
EUR 469.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

20-abx322517
  • EUR 526.80
  • EUR 393.60
  • 100 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx235048-100ug 100 ug
EUR 610.8

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx131298
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx131299
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx113712
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Mccc2 ORF Vector (Rat) (pORF)

ORF070367 1.0 ug DNA
EUR 607.2

MCCC2 ORF Vector (Human) (pORF)

ORF006320 1.0 ug DNA
EUR 114

MCCC2 ORF Vector (Human) (pORF)

ORF006321 1.0 ug DNA
EUR 114

Mccc2 ORF Vector (Mouse) (pORF)

ORF049931 1.0 ug DNA
EUR 607.2

MCCC2 ELISA Kit (Human) (OKCD00916)

OKCD00916 96 Wells
EUR 997.2
Description: Description of target: Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.9"Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC)."_x005F_x005F_x000D_Chu C.H., Cheng D._x005F_x005F_x000D_Protein Expr. Purif. 53:421-427(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.141 ng/mL

Mccc2 sgRNA CRISPR Lentivector set (Rat)

K7573601 3 x 1.0 ug
EUR 406.8

MCCC2 sgRNA CRISPR Lentivector set (Human)

K1278301 3 x 1.0 ug
EUR 406.8

Mccc2 sgRNA CRISPR Lentivector set (Mouse)

K3464301 3 x 1.0 ug
EUR 406.8

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human)

4-PAD690Hu01
  • EUR 313.20
  • EUR 3280.80
  • EUR 811.20
  • EUR 396.00
  • EUR 264.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse)

4-PAD690Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7573602 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7573603 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7573604 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 1)

K1278302 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 2)

K1278303 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 3)

K1278304 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3464302 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3464303 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3464304 1.0 ug DNA
EUR 184.8

MCCC2 Protein Vector (Human) (pPB-C-His)

PV025277 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-N-His)

PV025278 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-HA)

PV025279 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-His)

PV025280 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-C-His)

PV025281 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-N-His)

PV025282 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-HA)

PV025283 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-His)

PV025284 500 ng
EUR 394.8

MCCC2 Protein Vector (Mouse) (pPB-C-His)

PV199722 500 ng
EUR 723.6

MCCC2 Protein Vector (Mouse) (pPB-N-His)

PV199723 500 ng
EUR 723.6

MCCC2 Protein Vector (Mouse) (pPM-C-HA)

PV199724 500 ng
EUR 723.6

MCCC2 Protein Vector (Mouse) (pPM-C-His)

PV199725 500 ng
EUR 723.6

Recombinant Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

4-RPD690Hu01
  • EUR 631.80
  • EUR 292.80
  • EUR 2039.23
  • EUR 759.74
  • EUR 1399.49
  • EUR 498.00
  • EUR 4918.08
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Methylcrotonoyl Coenzyme A Carboxylase 2 expressed in: E.coli

Recombinant Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

4-RPD690Mu01
  • EUR 653.30
  • EUR 297.60
  • EUR 2119.87
  • EUR 786.62
  • EUR 1453.25
  • EUR 512.40
  • EUR 5119.68
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 expressed in: E.coli

MCCC2 3'UTR GFP Stable Cell Line

TU063103 1.0 ml
EUR 1825.2

Mccc2 3'UTR GFP Stable Cell Line

TU262953 1.0 ml Ask for price

Mccc2 3'UTR Luciferase Stable Cell Line

TU112987 1.0 ml Ask for price

Mccc2 3'UTR GFP Stable Cell Line

TU162987 1.0 ml Ask for price

MCCC2 Protein Vector (Rat) (pPB-C-His)

PV281466 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPB-N-His)

PV281467 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPM-C-HA)

PV281468 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPM-C-His)

PV281469 500 ng
EUR 723.6

MCCC2 3'UTR Luciferase Stable Cell Line

TU013103 1.0 ml
EUR 1825.2

Mccc2 3'UTR Luciferase Stable Cell Line

TU212953 1.0 ml Ask for price

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC

4-PAD690Hu01-APC
  • EUR 440.40
  • EUR 4297.20
  • EUR 1186.80
  • EUR 564.00
  • EUR 273.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Biotinylated

4-PAD690Hu01-Biotin
  • EUR 392.40
  • EUR 3220.80
  • EUR 939.60
  • EUR 483.60
  • EUR 270.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Cy3

4-PAD690Hu01-Cy3
  • EUR 536.40
  • EUR 5679.60
  • EUR 1532.40
  • EUR 702.00
  • EUR 315.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), FITC

4-PAD690Hu01-FITC
  • EUR 375.60
  • EUR 3460.80
  • EUR 973.20
  • EUR 475.20
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), HRP

4-PAD690Hu01-HRP
  • EUR 400.80
  • EUR 3744.00
  • EUR 1047.60
  • EUR 508.80
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), PE

4-PAD690Hu01-PE
  • EUR 375.60
  • EUR 3460.80
  • EUR 973.20
  • EUR 475.20
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC

4-PAD690Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Biotinylated

4-PAD690Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Cy3

4-PAD690Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), FITC

4-PAD690Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), HRP

4-PAD690Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), PE

4-PAD690Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Protein

20-abx650437
  • EUR 878.40
  • EUR 343.20
  • EUR 2749.20
  • EUR 1045.20
  • EUR 627.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Protein

20-abx650438
  • EUR 910.80
  • EUR 360.00
  • EUR 2848.80
  • EUR 1078.80
  • EUR 644.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV712203 1.0 ug DNA
EUR 379.2

MCCC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712207 1.0 ug DNA
EUR 379.2

MCCC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV712208 1.0 ug DNA
EUR 379.2

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV655585 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV655589 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV655590 1.0 ug DNA
EUR 818.4

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC-Cy7

4-PAD690Hu01-APC-Cy7
  • EUR 736.80
  • EUR 8450.40
  • EUR 2229.60
  • EUR 985.20
  • EUR 404.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC-Cy7

4-PAD690Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) CLIA Kit

20-abx494354
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

20-abx258564
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

4-SED690Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

ELISA kit for Human MCCC2 (Methylcrotonoyl Coenzyme A Carboxylase 2)

ELK5290 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Methylcrotonoyl Coenzyme A Carboxylase 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7573605 3 x 1.0 ug
EUR 451.2

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1278305 3 x 1.0 ug
EUR 451.2

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3464305 3 x 1.0 ug
EUR 451.2

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV712204 1.0 ug DNA
EUR 379.2

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV712205 1.0 ug DNA
EUR 448.8

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV712206 1.0 ug DNA
EUR 448.8

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV655586 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV655587 1.0 ug DNA
EUR 888

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV655588 1.0 ug DNA
EUR 888

ELISA kit for Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2)

KTE61696-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2)

KTE61696-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2)

KTE61696-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7573606 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7573607 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7573608 1.0 ug DNA
EUR 200.4

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1278306 1.0 ug DNA
EUR 200.4

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1278307 1.0 ug DNA
EUR 200.4

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1278308 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3464306 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3464307 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3464308 1.0 ug DNA
EUR 200.4

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 332.4
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
EUR 332.4

Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
EUR 332.4

Anti-Anti-SEPT11 Antibody antibody

STJ113941 100 µl
EUR 332.4

Anti-Anti-SEPT11 Antibody antibody

STJ114081 100 µl
EUR 332.4

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

STJ114828 100 µl
EUR 332.4

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
EUR 332.4
Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

STJ118549 100 µl
EUR 332.4

Anti-Anti-MARCH6 Antibody antibody

STJ118550 100 µl
EUR 332.4

Anti-Anti-MARCH7 Antibody antibody

STJ118752 100 µl
EUR 332.4

Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
EUR 332.4

Anti-Anti-SEPT2 Antibody antibody

STJ28365 100 µl
EUR 332.4

Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 332.4
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 332.4

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 332.4
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
EUR 332.4
Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
EUR 332.4
Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-DDB1 Antibody

A00333 100uL
EUR 546
Description: Rabbit Polyclonal DDB1 Antibody. Validated in IP and tested in Human, Mouse.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

AEA465Hu-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Competitive Enzyme-linked immunosorbent assay for Antibody Detection.detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in serum, plasma and other biological fluids.

Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) ELISA Kit

4-AEA465Hu
  • EUR 6837.60
  • EUR 3613.20
  • EUR 896.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Human Anti-Anti-Sperm Antibody Antibody (Anti-AsAb) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

ELISA kit for Human Anti-AsAb (Anti-Anti-Sperm Antibody Antibody)

ELK8071 1 plate of 96 wells
EUR 518.4
Description: A competitive Inhibition ELISA kit for detection of Anti-Anti-Sperm Antibody Antibody from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Anti-ZNF660 antibody

44742-100ul 100ul
EUR 302.4

Anti-ZNF660 antibody

44742-50ul 50ul
EUR 224.4

Anti-CCV Antibody

10-2789 1 mg
EUR 217.2
Description: Anti-Canine Corona Virus Antibody

Anti-CCV Antibody

10-2790 1 mg
EUR 217.2
Description: Anti-Canine Corona Virus Antibody

Anti-CDV antibody

10-2791 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody

10-2792 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody

10-2793 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CDV antibody

10-2794 1 mg
EUR 198
Description: Anti-Canine Distemper Virus antibody

Anti-CPV Antibody

10-2795 500 uig
EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-CPV Antibody

10-2796 500 ug
EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-CPV Antibody

10-2797 500 ug
EUR 165.6
Description: Anti-Canine Parvo Virus Antibody

Anti-C.CRP antibody

10-2798 500 ug
EUR 165.6
Description: Anti-Canine CRP antibody

Anti-C.CRP antibody

10-2799 500 ug
EUR 165.6
Description: Anti-Canine CRP antibody

Anti-CHW antibody

10-2800 1 mg
EUR 198
Description: Anti-Canine Heartworm antibody

The tactic provides the benefit of being carried out on stay cells, assuaging the necessity for a recombinant supply of the receptor. Moreover, time-resolved measurements, along with permitting the willpower of the affinity of the studied drug to its goal, give entry to the binding kinetics thereby offering a full characterization of the system. On this research, we additionally in contrast time-resolved LigandTracer information with end-point OkayD willpower from circulation cytometry experiments and hypothesize that discrepancies between these two approaches, once they exist, typically come from circulation cytometry titration curves being acquired previous to full equilibration of the system.

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