Highly sensitive detection of antibody nonspecific interactions using flow cytometry

The quickly evolving nature of antibody drug improvement has resulted in applied sciences that generate huge numbers (a whole bunch to hundreds) of lead antibody candidates throughout early discovery. These candidates have to be quickly pared all the way down to establish probably the most drug-like candidates for in-depth evaluation of their security and efficacy, which may solely be carried out on a restricted variety of antibodies attributable to time and useful resource necessities. One key biophysical property of profitable antibody therapeutics is excessive specificity, outlined as low ranges of nonspecific binding or polyspecificity.

Though there was some progress in creating assays for detecting antibody polyspecificity, most of those assays are restricted by poor sensitivity or assay codecs that require proprietary antibody floor show strategies, and a few of these assays use advanced and poorly outlined polyspecificity reagents. Right here we report the PolySpecificity Particle (PSP) assay, a delicate circulation cytometry assay for evaluating antibody nonspecific interactions that overcomes earlier limitations and can be utilized for evaluating various sorts of IgGs, multispecific antibodies and Fc-fusion proteins. Our strategy makes use of micron-sized magnetic beads coated with Protein A to seize antibodies at extraordinarily dilute concentrations (<0.02 mg/mL).

Move cytometry evaluation of polyspecificity reagent binding to those conjugates ends in delicate detection of variations in nonspecific interactions for clinical-stage antibodies. Our PSP assay strongly discriminates between antibodies with totally different ranges of polyspecificity utilizing beforehand reported polyspecificity reagents which are both well-defined proteins or extremely advanced protein mixtures. Furthermore, we additionally discover {that a} distinctive reagent, particularly ovalbumin, ends in the very best assay sensitivity and specificity. Importantly, our assay is far more delicate than customary assays equivalent to ELISAs. We anticipate that our easy, delicate, and high-throughput PSP assay will speed up the event of protected and efficient antibody therapeutics.

Toxicity of Carbon Nanomaterials-In the direction of Dependable Viability Evaluation by way of New Method in Move Cytometry

The scope of software of carbon nanomaterials in biomedical, environmental and industrial fields is lately considerably growing. Since in vitro toxicity testing is the primary important step for any business utilization, it’s essential to have a dependable methodology to investigate the possibly dangerous results of carbon nanomaterials. Regardless that researchers already reported the interference of carbon nanomaterials with widespread toxicity assays, there’s nonetheless, sadly, a lot of research that neglect this reality. On this research, we investigated interference of 4 bio-promising carbon nanomaterials (graphene acid (GA), cyanographene (GCN), graphitic carbon nitride (g-C3N4) and carbon dots (QCDs)) in generally used LIVE/DEAD assay.

When a regular process was utilized, supplies prompted varied sorts of interference. Whereas positively charged g-C3N4 and QCDs induced false outcomes by means of the creation of free agglomerates and intrinsic fluorescence properties, negatively charged GA and GCN led to false indicators as a result of advanced quenching impact of the fluorescent dye of a LIVE/DEAD package. Thus, we developed a brand new strategy utilizing a particular gating technique primarily based on extra controls that efficiently overcame all sorts of interference and result in dependable ends in LIVE/DEAD assay.

We advise that the newly developed process ought to be a compulsory device for all in vitro circulation cytometry assays of any class of carbon nanomaterials. Imaging circulation cytometry permits for the quantitative evaluation of fluorescent indicators on the subcellular degree. Right here, we describe using a biosensor cell line, particularly, U2OS osteosarcoma cells outfitted with a fusion protein comprising monomeric pink fluorescent protein (mRFP), inexperienced fluorescent protein (GFP) and microtubule-associated proteins 1A/1B gentle chain 3B (greatest often called LC3), for the evaluation of autophagic flux by imaging circulation cytometry.

Highly sensitive detection of antibody nonspecific interactions using flow cytometry

We element all evaluation instruments required to tell apart autophagosomes (that emit each a pink and a inexperienced fluorescence) and autolysosomes (that emit a pink fluorescence, but lose the inexperienced fluorescent sign) and to quantitate autophagic flux in a handy style. Our information, nevertheless, present that information of the kinetics of the interplay permits to reconcile the info obtained by circulation cytometry and LigandTracer and show the complementarity of those two strategies.

Okay D willpower from time-resolved experiments on stay cells with LigandTracer and reconciliation with end-point circulation cytometry measurements

Design of next-generation therapeutics comes with new challenges and emulates expertise and strategies to satisfy them. Characterizing the binding of both pure ligands or therapeutic proteins to cell-surface receptors, for which related recombinant variations could not exist, represents certainly one of these challenges. Right here we report the characterization of the interplay of 5 totally different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate goal receptors utilizing LigandTracer.

MCCC2 Antibody

1-CSB-PA013573GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against MCCC2. Recognizes MCCC2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

MCCC2 Polyclonal Antibody

31405-100ul 100ul
EUR 302.4

MCCC2 Polyclonal Antibody

31405-50ul 50ul
EUR 224.4

MCCC2 Polyclonal Antibody

28858-100ul 100ul
EUR 302.4

MCCC2 Polyclonal Antibody

28858-50ul 50ul
EUR 224.4

Polyclonal MCCC2 Antibody (Center)

APR10893G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MCCC2 (Center). This antibody is tested and proven to work in the following applications:

[One Step] MCCC2 Antibody Kit

RK05665 50 ul
EUR 288

MCCC2 Polyclonal Conjugated Antibody

C31405 100ul
EUR 476.4

MCCC2 Polyclonal Conjugated Antibody

C28858 100ul
EUR 476.4

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx235048-100ug 100 ug
EUR 610.8

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

20-abx322517
  • EUR 526.80
  • EUR 393.60
  • 100 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx030686-400ul 400 ul
EUR 627.6

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx030686-80l 80 µl
EUR 343.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx037012-100ug 100 ug
EUR 469.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx033068-400ul 400 ul
EUR 627.6

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx033068-80l 80 µl
EUR 343.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

20-abx006595
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx113712
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx131298
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx131299
  • EUR 543.60
  • EUR 159.60
  • EUR 1562.40
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mccc2/ Rat Mccc2 ELISA Kit

ELI-48233r 96 Tests
EUR 1063.2

MCCC2 siRNA

20-abx903187
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

MCCC2 siRNA

20-abx923707
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

MCCC2 siRNA

20-abx923708
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

anti- MCCC1 antibody

FNab05047 100µg
EUR 658.5
Description: Antibody raised against MCCC1

MCCC2 Rabbit pAb

A15181-100ul 100 ul
EUR 369.6

MCCC2 Rabbit pAb

A15181-200ul 200 ul
EUR 550.8

MCCC2 Rabbit pAb

A15181-20ul 20 ul
EUR 219.6

MCCC2 Rabbit pAb

A15181-50ul 50 ul
EUR 267.6

MCCC2 Rabbit pAb

A7990-100ul 100 ul
EUR 369.6

MCCC2 Rabbit pAb

A7990-200ul 200 ul
EUR 550.8

MCCC2 Rabbit pAb

A7990-20ul 20 ul
EUR 219.6

MCCC2 Rabbit pAb

A7990-50ul 50 ul
EUR 267.6

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human)

4-PAD690Hu01
  • EUR 313.20
  • EUR 3280.80
  • EUR 811.20
  • EUR 396.00
  • EUR 264.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse)

4-PAD690Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

MCCC2 cloning plasmid

CSB-CL867199HU1-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the MCCC2 gene.

MCCC2 cloning plasmid

CSB-CL867199HU2-10ug 10ug
EUR 451.2
Description: A cloning plasmid for the MCCC2 gene.

MCCC2 Blocking Peptide

DF12655-BP 1mg
EUR 234

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC

4-PAD690Hu01-APC
  • EUR 440.40
  • EUR 4297.20
  • EUR 1186.80
  • EUR 564.00
  • EUR 273.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Cy3

4-PAD690Hu01-Cy3
  • EUR 536.40
  • EUR 5679.60
  • EUR 1532.40
  • EUR 702.00
  • EUR 315.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), HRP

4-PAD690Hu01-HRP
  • EUR 400.80
  • EUR 3744.00
  • EUR 1047.60
  • EUR 508.80
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), PE

4-PAD690Hu01-PE
  • EUR 375.60
  • EUR 3460.80
  • EUR 973.20
  • EUR 475.20
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC

4-PAD690Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Cy3

4-PAD690Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), HRP

4-PAD690Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), PE

4-PAD690Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), FITC

4-PAD690Hu01-FITC
  • EUR 375.60
  • EUR 3460.80
  • EUR 973.20
  • EUR 475.20
  • EUR 242.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), FITC

4-PAD690Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC.

Mouse Mccc2 ELISA KIT

ELI-46423m 96 Tests
EUR 1038

MCCC2 ELISA KIT|Human

EF000692 96 Tests
EUR 826.8

Human MCCC2 ELISA KIT

ELI-15862h 96 Tests
EUR 988.8

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Biotinylated

4-PAD690Hu01-Biotin
  • EUR 392.40
  • EUR 3220.80
  • EUR 939.60
  • EUR 483.60
  • EUR 270.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Biotinylated

4-PAD690Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin.

Rat MCCC2 shRNA Plasmid

20-abx990287
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC-Cy7

4-PAD690Hu01-APC-Cy7
  • EUR 736.80
  • EUR 8450.40
  • EUR 2229.60
  • EUR 985.20
  • EUR 404.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC-Cy7

4-PAD690Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7.

Human MCCC2 shRNA Plasmid

20-abx961908
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse MCCC2 shRNA Plasmid

20-abx978856
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

MCCC2 Recombinant Protein (Rat)

RP211097 100 ug Ask for price

MCCC2 Recombinant Protein (Human)

RP018958 100 ug Ask for price

MCCC2 Recombinant Protein (Human)

RP018961 100 ug Ask for price

MCCC2 Recombinant Protein (Mouse)

RP149789 100 ug Ask for price

Mccc2 ORF Vector (Rat) (pORF)

ORF070367 1.0 ug DNA
EUR 607.2

MCCC2 ORF Vector (Human) (pORF)

ORF006320 1.0 ug DNA
EUR 114

MCCC2 ORF Vector (Human) (pORF)

ORF006321 1.0 ug DNA
EUR 114

Mccc2 ORF Vector (Mouse) (pORF)

ORF049931 1.0 ug DNA
EUR 607.2

MCCC2 ELISA Kit (Human) (OKCD00916)

OKCD00916 96 Wells
EUR 997.2
Description: Description of target: Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.9"Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC)."_x005F_x005F_x000D_Chu C.H., Cheng D._x005F_x005F_x000D_Protein Expr. Purif. 53:421-427(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.141 ng/mL

Mccc2 sgRNA CRISPR Lentivector set (Rat)

K7573601 3 x 1.0 ug
EUR 406.8

Mccc2 3'UTR GFP Stable Cell Line

TU162987 1.0 ml Ask for price

MCCC2 3'UTR GFP Stable Cell Line

TU063103 1.0 ml
EUR 1825.2

Mccc2 3'UTR GFP Stable Cell Line

TU262953 1.0 ml Ask for price

MCCC1 antibody

70R-18435 50 ul
EUR 522
Description: Rabbit polyclonal MCCC1 antibody

MCCC1 Antibody

47317-100ul 100ul
EUR 302.4

MCCC1 Antibody

1-CSB-PA853497EA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

MCCC1 Antibody

1-CSB-PA853497ESR1HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IP; Recommended dilution: IHC:1:20-1:200, IP:1:200-1:2000

MCCC1 Antibody

1-CSB-PA853497ESR2HU
  • EUR 266.40
  • EUR 402.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

MCCC1 Antibody

1-CSB-PA013572GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

MCCC2 sgRNA CRISPR Lentivector set (Human)

K1278301 3 x 1.0 ug
EUR 406.8

Mccc2 sgRNA CRISPR Lentivector set (Mouse)

K3464301 3 x 1.0 ug
EUR 406.8

MCCC2 Protein Vector (Rat) (pPM-C-HA)

PV281468 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPB-C-His)

PV281466 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPB-N-His)

PV281467 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPM-C-His)

PV281469 500 ng
EUR 723.6

MCCC2 Protein Vector (Human) (pPM-C-HA)

PV025279 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-HA)

PV025283 500 ng
EUR 394.8

MCCC2 Protein Vector (Mouse) (pPM-C-HA)

PV199724 500 ng
EUR 723.6

MCCC2 Protein Vector (Human) (pPB-C-His)

PV025277 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-N-His)

PV025278 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-His)

PV025280 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-C-His)

PV025281 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-N-His)

PV025282 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-His)

PV025284 500 ng
EUR 394.8

MCCC2 Protein Vector (Mouse) (pPB-C-His)

PV199722 500 ng
EUR 723.6

MCCC2 Protein Vector (Mouse) (pPB-N-His)

PV199723 500 ng
EUR 723.6

MCCC2 Protein Vector (Mouse) (pPM-C-His)

PV199725 500 ng
EUR 723.6

Mccc2 3'UTR Luciferase Stable Cell Line

TU112987 1.0 ml Ask for price

Mccc2 3'UTR Luciferase Stable Cell Line

TU212953 1.0 ml Ask for price

MCCC2 3'UTR Luciferase Stable Cell Line

TU013103 1.0 ml
EUR 1825.2

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7573602 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7573603 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7573604 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 1)

K1278302 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 2)

K1278303 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 3)

K1278304 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3464302 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3464303 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3464304 1.0 ug DNA
EUR 184.8

MCCC1 Antibody, HRP conjugated

1-CSB-PA853497EB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

MCCC1 Antibody, FITC conjugated

1-CSB-PA853497EC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

MCCC1 Antibody, Biotin conjugated

1-CSB-PA853497ED01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV655585 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV655589 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)

LV712203 1.0 ug DNA
EUR 379.2

MCCC2 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)

LV712207 1.0 ug DNA
EUR 379.2

MCCC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV655590 1.0 ug DNA
EUR 818.4

Anti-MCCC2 antibody

PAab05048 100 ug
EUR 463.2

Anti-MCCC2 antibody

STJ110297 100 µl
EUR 332.4
Description: This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Anti-MCCC2 antibody

STJ117375 100 µl
EUR 332.4
Description: This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Recombinant Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

4-RPD690Hu01
  • EUR 631.80
  • EUR 292.80
  • EUR 2039.23
  • EUR 759.74
  • EUR 1399.49
  • EUR 498.00
  • EUR 4918.08
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Methylcrotonoyl Coenzyme A Carboxylase 2 expressed in: E.coli

Recombinant Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

4-RPD690Mu01
  • EUR 653.30
  • EUR 297.60
  • EUR 2119.87
  • EUR 786.62
  • EUR 1453.25
  • EUR 512.40
  • EUR 5119.68
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 expressed in: E.coli

MCCC2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)

LV712208 1.0 ug DNA
EUR 379.2

MCCC1 Conjugated Antibody

C47317 100ul
EUR 476.4

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Protein

20-abx650437
  • EUR 878.40
  • EUR 343.20
  • EUR 2749.20
  • EUR 1045.20
  • EUR 627.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Protein

20-abx650438
  • EUR 910.80
  • EUR 360.00
  • EUR 2848.80
  • EUR 1078.80
  • EUR 644.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Monoclonal MCCC1 Antibody (monoclonal) (M01), Clone: 2G8

APR08376G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human MCCC1 (monoclonal) (M01). The antibodies are raised in mouse and are from clone 2G8. This antibody is applicable in WB, E

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7573605 3 x 1.0 ug
EUR 451.2

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) CLIA Kit

20-abx494354
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

20-abx258564
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

SED690Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in serum, plasma, tissue homogenates and other biological fluids.

Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) ELISA Kit

4-SED690Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K1278305 3 x 1.0 ug
EUR 451.2

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3464305 3 x 1.0 ug
EUR 451.2

Methylcrotonoyl-CoA Carboxylase 1 (MCCC1) Antibody

20-abx135938
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 1 (MCCC1) Antibody

abx235047-100ug 100 ug
EUR 610.8

Methylcrotonoyl-CoA Carboxylase 1 (MCCC1) Antibody

20-abx321317
  • EUR 526.80
  • EUR 393.60
  • 100 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 1 (MCCC1) Antibody

20-abx322082
  • EUR 526.80
  • EUR 393.60
  • 100 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 1 (MCCC1) Antibody

abx037719-100ug 100 ug
EUR 469.2

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Antibody

20-abx113711
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Antibody

20-abx131297
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7573606 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7573607 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7573608 1.0 ug DNA
EUR 200.4

ELISA kit for Human MCCC2 (Methylcrotonoyl Coenzyme A Carboxylase 2)

ELK5290 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Methylcrotonoyl Coenzyme A Carboxylase 2 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K1278306 1.0 ug DNA
EUR 200.4

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K1278307 1.0 ug DNA
EUR 200.4

MCCC2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K1278308 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3464306 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3464307 1.0 ug DNA
EUR 200.4

Mccc2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3464308 1.0 ug DNA
EUR 200.4

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV655586 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV712204 1.0 ug DNA
EUR 379.2

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV655587 1.0 ug DNA
EUR 888

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV655588 1.0 ug DNA
EUR 888

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV712205 1.0 ug DNA
EUR 448.8

MCCC2 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV712206 1.0 ug DNA
EUR 448.8

ELISA kit for Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2)

KTE61696-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2)

KTE61696-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2)

KTE61696-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial (MCCC2) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse)

4-PAD689Mu01
  • EUR 301.20
  • EUR 3091.20
  • EUR 768.00
  • EUR 379.20
  • EUR 258.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1)

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse), APC

4-PAD689Mu01-APC
  • EUR 421.20
  • EUR 4038.00
  • EUR 1122.00
  • EUR 538.80
  • EUR 266.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1). This antibody is labeled with APC.

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse), Cy3

4-PAD689Mu01-Cy3
  • EUR 512.40
  • EUR 5334.00
  • EUR 1446.00
  • EUR 668.40
  • EUR 304.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1). This antibody is labeled with Cy3.

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse), HRP

4-PAD689Mu01-HRP
  • EUR 385.20
  • EUR 3519.60
  • EUR 992.40
  • EUR 486.00
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1). This antibody is labeled with HRP.

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse), PE

4-PAD689Mu01-PE
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1). This antibody is labeled with PE.

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse), FITC

4-PAD689Mu01-FITC
  • EUR 361.20
  • EUR 3254.40
  • EUR 921.60
  • EUR 454.80
  • EUR 236.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1). This antibody is labeled with FITC.

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse), APC-Cy7

4-PAD689Mu01-APC-Cy7
  • EUR 699.60
  • EUR 7932.00
  • EUR 2100.00
  • EUR 933.60
  • EUR 388.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1). This antibody is labeled with APC-Cy7.

anti-MCCC2

YF-PA20435 50 ul
EUR 435.6
Description: Mouse polyclonal to MCCC2

anti-MCCC2

YF-PA20436 50 ug
EUR 435.6
Description: Mouse polyclonal to MCCC2

anti-MCCC2

YF-PA20437 100 ug
EUR 483.6
Description: Rabbit polyclonal to MCCC2

anti-MCCC2

YF-PA26541 50 ul
EUR 400.8
Description: Mouse polyclonal to MCCC2

Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1) Polyclonal Antibody (Mouse), Biotinylated

4-PAD689Mu01-Biotin
  • EUR 379.20
  • EUR 3031.20
  • EUR 892.80
  • EUR 464.40
  • EUR 265.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 1 (MCCC1). This antibody is labeled with Biotin.

Anti-MCCC2 (2B3)

YF-MA19192 100 ug
EUR 435.6
Description: Mouse monoclonal to MCCC2

Mccc1/ Rat Mccc1 ELISA Kit

ELI-22768r 96 Tests
EUR 1063.2

MCCC1 siRNA

20-abx903186
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

MCCC1 siRNA

20-abx923705
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

MCCC1 siRNA

20-abx923706
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

MCCC1 Rabbit pAb

A10020-100ul 100 ul
EUR 369.6

MCCC1 Rabbit pAb

A10020-200ul 200 ul
EUR 550.8

MCCC1 Rabbit pAb

A10020-20ul 20 ul
EUR 219.6

MCCC1 Rabbit pAb

A10020-50ul 50 ul
EUR 267.6

MCCC1 ELISA KIT|Human

EF000691 96 Tests
EUR 826.8

Human MCCC1 ELISA KIT

ELI-39149h 96 Tests
EUR 988.8

Mouse Mccc1 ELISA KIT

ELI-20526m 96 Tests
EUR 1038

Mccc1 ORF Vector (Rat) (pORF)

ORF070366 1.0 ug DNA
EUR 607.2

Rat MCCC1 shRNA Plasmid

20-abx988730
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

MCCC1 cloning plasmid

CSB-CL853497HU-10ug 10ug
EUR 568.8
Description: A cloning plasmid for the MCCC1 gene.

MCCC1 ORF Vector (Human) (pORF)

ORF006319 1.0 ug DNA
EUR 114

Mccc1 ORF Vector (Mouse) (pORF)

ORF049930 1.0 ug DNA
EUR 607.2

Mccc1 3'UTR GFP Stable Cell Line

TU162986 1.0 ml Ask for price

MCCC1 3'UTR GFP Stable Cell Line

TU063102 1.0 ml
EUR 1825.2

Mccc1 3'UTR GFP Stable Cell Line

TU262952 1.0 ml Ask for price

Mouse MCCC1 shRNA Plasmid

20-abx977639
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

The tactic provides the benefit of being carried out on stay cells, assuaging the necessity for a recombinant supply of the receptor. Moreover, time-resolved measurements, along with permitting the willpower of the affinity of the studied drug to its goal, give entry to the binding kinetics thereby offering a full characterization of the system. On this research, we additionally in contrast time-resolved LigandTracer information with end-point OkayD willpower from circulation cytometry experiments and hypothesize that discrepancies between these two approaches, once they exist, typically come from circulation cytometry titration curves being acquired previous to full equilibration of the system.

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