The quickly evolving nature of antibody drug improvement has resulted in applied sciences that generate huge numbers (a whole bunch to hundreds) of lead antibody candidates throughout early discovery. These candidates have to be quickly pared all the way down to establish probably the most drug-like candidates for in-depth evaluation of their security and efficacy, which may solely be carried out on a restricted variety of antibodies attributable to time and useful resource necessities. One key biophysical property of profitable antibody therapeutics is excessive specificity, outlined as low ranges of nonspecific binding or polyspecificity.
Though there was some progress in creating assays for detecting antibody polyspecificity, most of those assays are restricted by poor sensitivity or assay codecs that require proprietary antibody floor show strategies, and a few of these assays use advanced and poorly outlined polyspecificity reagents. Right here we report the PolySpecificity Particle (PSP) assay, a delicate circulation cytometry assay for evaluating antibody nonspecific interactions that overcomes earlier limitations and can be utilized for evaluating various sorts of IgGs, multispecific antibodies and Fc-fusion proteins. Our strategy makes use of micron-sized magnetic beads coated with Protein A to seize antibodies at extraordinarily dilute concentrations (<0.02 mg/mL).
Move cytometry evaluation of polyspecificity reagent binding to those conjugates ends in delicate detection of variations in nonspecific interactions for clinical-stage antibodies. Our PSP assay strongly discriminates between antibodies with totally different ranges of polyspecificity utilizing beforehand reported polyspecificity reagents which are both well-defined proteins or extremely advanced protein mixtures. Furthermore, we additionally discover {that a} distinctive reagent, particularly ovalbumin, ends in the very best assay sensitivity and specificity. Importantly, our assay is far more delicate than customary assays equivalent to ELISAs. We anticipate that our easy, delicate, and high-throughput PSP assay will speed up the event of protected and efficient antibody therapeutics.
Toxicity of Carbon Nanomaterials-In the direction of Dependable Viability Evaluation by way of New Method in Move Cytometry
The scope of software of carbon nanomaterials in biomedical, environmental and industrial fields is lately considerably growing. Since in vitro toxicity testing is the primary important step for any business utilization, it’s essential to have a dependable methodology to investigate the possibly dangerous results of carbon nanomaterials. Regardless that researchers already reported the interference of carbon nanomaterials with widespread toxicity assays, there’s nonetheless, sadly, a lot of research that neglect this reality. On this research, we investigated interference of 4 bio-promising carbon nanomaterials (graphene acid (GA), cyanographene (GCN), graphitic carbon nitride (g-C3N4) and carbon dots (QCDs)) in generally used LIVE/DEAD assay.
When a regular process was utilized, supplies prompted varied sorts of interference. Whereas positively charged g-C3N4 and QCDs induced false outcomes by means of the creation of free agglomerates and intrinsic fluorescence properties, negatively charged GA and GCN led to false indicators as a result of advanced quenching impact of the fluorescent dye of a LIVE/DEAD package. Thus, we developed a brand new strategy utilizing a particular gating technique primarily based on extra controls that efficiently overcame all sorts of interference and result in dependable ends in LIVE/DEAD assay.
We advise that the newly developed process ought to be a compulsory device for all in vitro circulation cytometry assays of any class of carbon nanomaterials. Imaging circulation cytometry permits for the quantitative evaluation of fluorescent indicators on the subcellular degree. Right here, we describe using a biosensor cell line, particularly, U2OS osteosarcoma cells outfitted with a fusion protein comprising monomeric pink fluorescent protein (mRFP), inexperienced fluorescent protein (GFP) and microtubule-associated proteins 1A/1B gentle chain 3B (greatest often called LC3), for the evaluation of autophagic flux by imaging circulation cytometry.
We element all evaluation instruments required to tell apart autophagosomes (that emit each a pink and a inexperienced fluorescence) and autolysosomes (that emit a pink fluorescence, but lose the inexperienced fluorescent sign) and to quantitate autophagic flux in a handy style. Our information, nevertheless, present that information of the kinetics of the interplay permits to reconcile the info obtained by circulation cytometry and LigandTracer and show the complementarity of those two strategies.
Okay D willpower from time-resolved experiments on stay cells with LigandTracer and reconciliation with end-point circulation cytometry measurements
Design of next-generation therapeutics comes with new challenges and emulates expertise and strategies to satisfy them. Characterizing the binding of both pure ligands or therapeutic proteins to cell-surface receptors, for which related recombinant variations could not exist, represents certainly one of these challenges. Right here we report the characterization of the interplay of 5 totally different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate goal receptors utilizing LigandTracer.
MCCC2 Antibody |
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DF12655-100ul | Affinity Biosciences | 100ul | EUR 280 |
MCCC2 Antibody |
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DF12655-200ul | Affinity Biosciences | 200ul | EUR 350 |
MCCC2 antibody |
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E39-05048 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
MCCC2 Antibody |
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E306903 | EnoGene | 100ug/200ul | EUR 275 |
Description: Available in various conjugation types. |
MCCC2 antibody |
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70R-18436 | Fitzgerald | 50 ul | EUR 289 |
Description: Rabbit polyclonal MCCC2 antibody |
MCCC2 Antibody |
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1-CSB-PA867199ESR1HU | Cusabio |
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Description: A polyclonal antibody against MCCC2. Recognizes MCCC2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
MCCC2 Polyclonal Antibody |
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28858 | SAB | 100ul | EUR 439 |
MCCC2 Polyclonal Antibody |
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28858-100ul | SAB | 100ul | EUR 302.4 |
MCCC2 Polyclonal Antibody |
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28858-50ul | SAB | 50ul | EUR 224.4 |
MCCC2 Polyclonal Antibody |
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31405 | SAB | 100ul | EUR 439 |
MCCC2 Polyclonal Antibody |
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31405-100ul | SAB | 100ul | EUR 302.4 |
MCCC2 Polyclonal Antibody |
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31405-50ul | SAB | 50ul | EUR 224.4 |
MCCC2 Polyclonal Antibody |
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E915181 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
MCCC2 Polyclonal Antibody |
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E-AB-66453-120uL | Elabscience Biotech | 120uL | EUR 320 |
Description: Unconjugated |
MCCC2 Polyclonal Antibody |
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E-AB-66453-200uL | Elabscience Biotech | 200uL | EUR 530 |
Description: Unconjugated |
MCCC2 Polyclonal Antibody |
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E-AB-66453-60uL | Elabscience Biotech | 60uL | EUR 200 |
Description: Unconjugated |
MCCC2 Polyclonal Antibody |
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E-AB-66453-each | Elabscience Biotech | each | Ask for price |
Description: Unconjugated |
OACD00477-1ML - MCCC2 Antibody |
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OACD00477-1ML | Aviva Systems Biology | 1ml | EUR 739 |
OACD00478-1ML - MCCC2 Antibody |
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OACD00478-1ML | Aviva Systems Biology | 1ml | EUR 649 |
OACA08635-50UL - MCCC2 Antibody |
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OACA08635-50UL | Aviva Systems Biology | 50ul | EUR 189 |
OAAN02555-50UL - MCCC2 Antibody |
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OAAN02555-50UL | Aviva Systems Biology | 50ul | EUR 149 |
Polyclonal MCCC2 Antibody (Center) |
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APR10893G | Leading Biology | 0.1ml | EUR 633.6 |
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MCCC2 (Center). This antibody is tested and proven to work in the following applications: |
OACA08635-100UL - MCCC2 Antibody |
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OACA08635-100UL | Aviva Systems Biology | 100ul | EUR 230 |
OAGA01973-100UL - MCCC2 Antibody |
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OAGA01973-100UL | Aviva Systems Biology | 100ul | EUR 369 |
OAGA05503-100UL - MCCC2 Antibody |
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OAGA05503-100UL | Aviva Systems Biology | 100ul | EUR 369 |
OAAN02555-100UL - MCCC2 Antibody |
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OAAN02555-100UL | Aviva Systems Biology | 100ul | EUR 249 |
OAAN02555-200UL - MCCC2 Antibody |
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OAAN02555-200UL | Aviva Systems Biology | 200ul | EUR 389 |
OAAL00786-100UG - MCCC2 Antibody |
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OAAL00786-100UG | Aviva Systems Biology | 100ug | EUR 349 |
OACD00477-100UL - MCCC2 Antibody |
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OACD00477-100UL | Aviva Systems Biology | 100ul | EUR 209 |
OACD00478-100UL - MCCC2 Antibody |
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OACD00478-100UL | Aviva Systems Biology | 100ul | EUR 199 |
[One Step] MCCC2 Antibody Kit |
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RK05665 | Abclonal | 50 ul | EUR 288 |
MCCC2 Polyclonal Conjugated Antibody |
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C31405 | SAB | 100ul | EUR 476.4 |
MCCC2 Polyclonal Conjugated Antibody |
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C28858 | SAB | 100ul | EUR 476.4 |
MCCC2 (Center) rabbit polyclonal antibody |
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AP17553PU-N | Origene Technologies GmbH | 400 µl | Ask for price |
OAAB04039-400UL - MCCC2 Antibody - middle region |
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OAAB04039-400UL | Aviva Systems Biology | 400ul | EUR 409 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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20-abx322517 | Abbexa |
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Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx037012-100ug | Abbexa | 100 ug | EUR 469.2 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx235048-100ug | Abbexa | 100 ug | EUR 610.8 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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20-abx006595 | Abbexa |
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Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx030686-400ul | Abbexa | 400 ul | EUR 627.6 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx030686-80l | Abbexa | 80 µl | EUR 343.2 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx033068-400ul | Abbexa | 400 ul | EUR 627.6 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx033068-80l | Abbexa | 80 µl | EUR 343.2 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx006595-100g | Abbexa | 100 µg | EUR 275 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx006595-10g | Abbexa | 10 µg | EUR 175 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx006595-200g | Abbexa | 200 µg | EUR 400 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx235048-100g | Abbexa | 100 µg | EUR 350 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx037012-1096tests | Abbexa | 10 × 96 tests | Ask for price |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx037012-596tests | Abbexa | 5 × 96 tests | Ask for price |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx037012-96tests | Abbexa | 96 tests | EUR 337.5 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx030686-400l | Abbexa | 400 µl | EUR 518.75 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx033068-400l | Abbexa | 400 µl | EUR 518.75 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx322517-100g | Abbexa | 100 µg | EUR 350 |
Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody |
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abx322517-50g | Abbexa | 50 µg | EUR 250 |
ARP82826_P050-25UL - MCCC2 Antibody - C-terminal region |
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ARP82826_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
ARP46313_P050-25UL - MCCC2 Antibody - C-terminal region |
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ARP46313_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
ARP46314_P050-25UL - MCCC2 Antibody - N-terminal region |
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ARP46314_P050-25UL | Aviva Systems Biology | 25ul | EUR 99 |
OAAB14516-400UL - MCCC2 Antibody - C-terminal region |
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OAAB14516-400UL | Aviva Systems Biology | 400ul | EUR 389 |
ARP82826_P050 - MCCC2 Antibody - C-terminal region (ARP82826_P050) |
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ARP82826_P050 | Aviva Systems Biology | 100ul | EUR 389 |
ARP46313_P050 - MCCC2 Antibody - C-terminal region (ARP46313_P050) |
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ARP46313_P050 | Aviva Systems Biology | 100ul | EUR 389 |
ARP46314_P050 - MCCC2 Antibody - N-terminal region (ARP46314_P050) |
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ARP46314_P050 | Aviva Systems Biology | 100ul | EUR 389 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody |
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20-abx131298 | Abbexa |
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Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody |
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20-abx131299 | Abbexa |
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Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody |
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20-abx113712 | Abbexa |
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Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody |
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abx113712-100l | Abbexa | 100 µl | EUR 612.5 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody |
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abx131298-05mg | Abbexa | 0.5 mg | EUR 275 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody |
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abx131299-05mg | Abbexa | 0.5 mg | EUR 287.5 |
Mccc2/ Rat Mccc2 ELISA Kit |
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ELI-48233r | Lifescience Market | 96 Tests | EUR 1063.2 |
MCCC2 |
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E8ER1912-86 | EnoGene | 100ul | EUR 275 |
Description: Available in various conjugation types. |
MCCC2 |
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CSB-CL867199HU1 | Cusabio | 10 μg plasmid + 200μl Glycerol | Ask for price |
MCCC2 |
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CSB-CL867199HU2 | Cusabio | 10 μg plasmid + 200μl Glycerol | Ask for price |
MCCC2 siRNA |
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20-abx923707 | Abbexa |
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MCCC2 siRNA |
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20-abx923708 | Abbexa |
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MCCC2 siRNA |
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20-abx903187 | Abbexa |
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Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody |
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CAU23159-100ul | Biomatik Corporation | 100ul | EUR 241.9 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody |
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CAU23159-200ul | Biomatik Corporation | 200ul | EUR 302.4 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody |
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CAU23160-100ul | Biomatik Corporation | 100ul | EUR 258.2 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody |
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CAU23160-200ul | Biomatik Corporation | 200ul | EUR 322.6 |
Polyclonal Antibody to Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) |
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PAD690Hu01 | Cloud-Clone | 100ul | EUR 269 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human) |
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4-PAD690Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse) |
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4-PAD690Mu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) |
MCCC2 Rabbit pAb |
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E2507990 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
MCCC2 Rabbit pAb |
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E2515181 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
MCCC2 Rabbit pAb |
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A15181-100ul | Abclonal | 100 ul | EUR 369.6 |
MCCC2 Rabbit pAb |
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A15181-200ul | Abclonal | 200 ul | EUR 550.8 |
MCCC2 Rabbit pAb |
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A15181-20ul | Abclonal | 20 ul | EUR 219.6 |
MCCC2 Rabbit pAb |
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A15181-50ul | Abclonal | 50 ul | EUR 267.6 |
MCCC2 Rabbit pAb |
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A7990-100ul | Abclonal | 100 ul | EUR 369.6 |
MCCC2 Rabbit pAb |
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A7990-200ul | Abclonal | 200 ul | EUR 550.8 |
MCCC2 Rabbit pAb |
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A7990-20ul | Abclonal | 20 ul | EUR 219.6 |
MCCC2 Rabbit pAb |
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A7990-50ul | Abclonal | 50 ul | EUR 267.6 |
MCCC2 cloning plasmid |
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CSB-CL867199HU1-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the MCCC2 gene. |
MCCC2 cloning plasmid |
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CSB-CL867199HU2-10ug | Cusabio | 10ug | EUR 451.2 |
Description: A cloning plasmid for the MCCC2 gene. |
MCCC2 Blocking Peptide |
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DF12655-BP | Affbiotech | 1mg | EUR 234 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC |
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4-PAD690Hu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Cy3 |
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4-PAD690Hu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), HRP |
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4-PAD690Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), PE |
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4-PAD690Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC |
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4-PAD690Mu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Cy3 |
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4-PAD690Mu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), HRP |
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4-PAD690Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), PE |
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4-PAD690Mu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE. |
anti- MCCC1 antibody |
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FNab05047 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against MCCC1 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), FITC |
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4-PAD690Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), FITC |
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4-PAD690Mu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Biotinylated |
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4-PAD690Hu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Biotinylated |
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4-PAD690Mu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin. |
Mouse Mccc2 ELISA KIT |
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ELI-46423m | Lifescience Market | 96 Tests | EUR 1038 |
MCCC2 ELISA KIT|Human |
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EF000692 | Lifescience Market | 96 Tests | EUR 826.8 |
Human MCCC2 ELISA KIT |
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ELI-15862h | Lifescience Market | 96 Tests | EUR 988.8 |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC-Cy7 |
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4-PAD690Hu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7. |
Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC-Cy7 |
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4-PAD690Mu01-APC-Cy7 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7. |
MCCC2 (Center) Rabbit pAb |
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E2620575 | EnoGene | 100ul | EUR 225 |
Description: Available in various conjugation types. |
Rat MCCC2 shRNA Plasmid |
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20-abx990287 | Abbexa |
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Human MCCC2 shRNA Plasmid |
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20-abx961908 | Abbexa |
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Mouse MCCC2 shRNA Plasmid |
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20-abx978856 | Abbexa |
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MCCC2 Recombinant Protein (Rat) |
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RP211097 | ABM | 100 ug | Ask for price |
MCCC2 Recombinant Protein (Mouse) |
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RP149789 | ABM | 100 ug | Ask for price |
MCCC2 Recombinant Protein (Human) |
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RP018958 | ABM | 100 ug | Ask for price |
MCCC2 Recombinant Protein (Human) |
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RP018961 | ABM | 100 ug | Ask for price |
Mccc2 ORF Vector (Rat) (pORF) |
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ORF070367 | ABM | 1.0 ug DNA | EUR 607.2 |
MCCC2 ORF Vector (Human) (pORF) |
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ORF006320 | ABM | 1.0 ug DNA | EUR 114 |
MCCC2 ORF Vector (Human) (pORF) |
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ORF006321 | ABM | 1.0 ug DNA | EUR 114 |
Mccc2 ORF Vector (Mouse) (pORF) |
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ORF049931 | ABM | 1.0 ug DNA | EUR 607.2 |
Human MCCC2 knockout cell line |
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ABC-KH9104 | AcceGen | 1 vial | Ask for price |
Description: Human MCCC2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human MCCC2 knockdown cell line |
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ABC-KD9104 | AcceGen | 1 vial | Ask for price |
Description: Human MCCC2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human MCCC2 Protein Lysate 20ug |
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IHUMCCC2PLLY20UG | Innovative research | each | EUR 213 |
Description: Human MCCC2 Protein Lysate 20ug |
MCCC2 ELISA Kit (Human) (OKCD00916) |
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OKCD00916 | Aviva Systems Biology | 96 Wells | EUR 997.2 |
Description: Description of target: Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.1 Publication
<p>Manually curated information for which there is published experimental evidence.</p>
<p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.9"Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC)."_x005F_x005F_x000D_Chu C.H., Cheng D._x005F_x005F_x000D_Protein Expr. Purif. 53:421-427(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.141 ng/mL |
OCOA08656-20UG - MCCC2 Protein Lysate |
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OCOA08656-20UG | Aviva Systems Biology | 20ug | EUR 169 |
MCCC2 Over-expression Lysate Product |
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GWB-C1A7F5 | GenWay Biotech | 0.1 mg | Ask for price |
OPCD00165-10UG - MCCC2 Recombinant Protein |
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OPCD00165-10UG | Aviva Systems Biology | 10ug | EUR 149 |
OPCD00165-50UG - MCCC2 Recombinant Protein |
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OPCD00165-50UG | Aviva Systems Biology | 50ug | EUR 369 |
OPCD00166-10UG - MCCC2 Recombinant Protein |
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OPCD00166-10UG | Aviva Systems Biology | 10ug | EUR 159 |
OPCD00166-50UG - MCCC2 Recombinant Protein |
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OPCD00166-50UG | Aviva Systems Biology | 50ug | EUR 389 |
OPCD00165-200UG - MCCC2 Recombinant Protein |
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OPCD00165-200UG | Aviva Systems Biology | 200ug | EUR 739 |
OPCD00166-200UG - MCCC2 Recombinant Protein |
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OPCD00166-200UG | Aviva Systems Biology | 200ug | EUR 769 |
MCCC2 (untagged)-Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein |
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SC125472 | Origene Technologies GmbH | 10 µg | Ask for price |
OKCD00916-96W - MCCC2 ELISA Kit (Human) |
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OKCD00916-96W | Aviva Systems Biology | 96Wells | EUR 675 |
Mccc2 sgRNA CRISPR Lentivector set (Rat) |
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K7573601 | ABM | 3 x 1.0 ug | EUR 406.8 |
MCCC2 3'UTR GFP Stable Cell Line |
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TU063103 | ABM | 1.0 ml | EUR 1825.2 |
Mccc2 3'UTR GFP Stable Cell Line |
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TU162987 | ABM | 1.0 ml | Ask for price |
Mccc2 3'UTR GFP Stable Cell Line |
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TU262953 | ABM | 1.0 ml | Ask for price |
MCCC2 sgRNA CRISPR Lentivector set (Human) |
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K1278301 | ABM | 3 x 1.0 ug | EUR 406.8 |
Mccc2 sgRNA CRISPR Lentivector set (Mouse) |
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K3464301 | ABM | 3 x 1.0 ug | EUR 406.8 |
MCCC2 (GFP-tagged) - Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein |
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RG209488 | Origene Technologies GmbH | 10 µg | Ask for price |
MCCC2 Protein Vector (Rat) (pPM-C-HA) |
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PV281468 | ABM | 500 ng | EUR 723.6 |
MCCC2 Protein Vector (Rat) (pPB-C-His) |
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PV281466 | ABM | 500 ng | EUR 723.6 |
MCCC2 Protein Vector (Rat) (pPB-N-His) |
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PV281467 | ABM | 500 ng | EUR 723.6 |
MCCC2 Protein Vector (Rat) (pPM-C-His) |
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PV281469 | ABM | 500 ng | EUR 723.6 |
MCCC2 Protein Vector (Human) (pPM-C-HA) |
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PV025279 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Human) (pPM-C-HA) |
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PV025283 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Mouse) (pPM-C-HA) |
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PV199724 | ABM | 500 ng | EUR 723.6 |
MCCC2 Protein Vector (Human) (pPB-C-His) |
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PV025277 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Human) (pPB-N-His) |
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PV025278 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Human) (pPM-C-His) |
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PV025280 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Human) (pPB-C-His) |
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PV025281 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Human) (pPB-N-His) |
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PV025282 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Human) (pPM-C-His) |
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PV025284 | ABM | 500 ng | EUR 394.8 |
MCCC2 Protein Vector (Mouse) (pPB-C-His) |
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PV199722 | ABM | 500 ng | EUR 723.6 |
MCCC2 Protein Vector (Mouse) (pPB-N-His) |
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PV199723 | ABM | 500 ng | EUR 723.6 |
MCCC2 Protein Vector (Mouse) (pPM-C-His) |
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PV199725 | ABM | 500 ng | EUR 723.6 |
Mccc2 3'UTR Luciferase Stable Cell Line |
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TU112987 | ABM | 1.0 ml | Ask for price |
MCCC2 3'UTR Luciferase Stable Cell Line |
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TU013103 | ABM | 1.0 ml | EUR 1825.2 |
Mccc2 3'UTR Luciferase Stable Cell Line |
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TU212953 | ABM | 1.0 ml | Ask for price |
MCCC2 (Myc-DDK-tagged)-Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein |
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RC209488 | Origene Technologies GmbH | 10 µg | Ask for price |
Mccc2 (untagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10ug) |
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MC219103 | Origene Technologies GmbH | 10 µg | Ask for price |
Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K7573602 | ABM | 1.0 ug DNA | EUR 184.8 |
Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K7573603 | ABM | 1.0 ug DNA | EUR 184.8 |
Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K7573604 | ABM | 1.0 ug DNA | EUR 184.8 |
Human MCCC2 Over-expressing Stable Cell Line |
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ABC-X1839 | AcceGen | 1 vial | Ask for price |
Description: Gentaur can provide custom lentiviral constructs expressing any genes of interest as long as it is less than about 3 kb. Lentiviral technology enables us to efficiently generate stable expression lines which are then selected for moderate or high expressers, depending on the experimental requirements. If you are interested in specific lentiviral DNA constructs or have further questions, please contact us to discuss the details. MCCC2 methylcrotonoyl-CoA carboxylase 2 (beta) [ Homo sapiens ] http://www.ncbi.nlm.nih.gov/gene/64087 |
MCCC2 sgRNA CRISPR Lentivector (Human) (Target 1) |
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K1278302 | ABM | 1.0 ug DNA | EUR 184.8 |
MCCC2 sgRNA CRISPR Lentivector (Human) (Target 2) |
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K1278303 | ABM | 1.0 ug DNA | EUR 184.8 |
MCCC2 sgRNA CRISPR Lentivector (Human) (Target 3) |
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K1278304 | ABM | 1.0 ug DNA | EUR 184.8 |
Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K3464302 | ABM | 1.0 ug DNA | EUR 184.8 |
Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K3464303 | ABM | 1.0 ug DNA | EUR 184.8 |
Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K3464304 | ABM | 1.0 ug DNA | EUR 184.8 |
MCCC1 Antibody |
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47317 | SAB | 100ul | EUR 319 |
MCCC1 Antibody |
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47317-100ul | SAB | 100ul | EUR 302.4 |
MCCC1 Antibody |
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1-CSB-PA013572GA01HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
MCCC1 Antibody |
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E047317 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
MCCC1 Antibody |
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E306902 | EnoGene | 100ug/200ul | EUR 275 |
Description: Available in various conjugation types. |
MCCC1 antibody |
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70R-18435 | Fitzgerald | 50 ul | EUR 289 |
Description: Rabbit polyclonal MCCC1 antibody |
MCCC1 Antibody |
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1-CSB-PA853497EA01HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200 |
MCCC1 Antibody |
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1-CSB-PA853497ESR1HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IP; Recommended dilution: IHC:1:20-1:200, IP:1:200-1:2000 |
MCCC1 Antibody |
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1-CSB-PA853497ESR2HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200 |
Mccc2 (GFP-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2) nuclear gene encoding mitochondrial protein, (10ug) |
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MG224804 | Origene Technologies GmbH | 10 µg | Ask for price |
AAP46313-100UG - MCCC2 Peptide - C-terminal region |
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AAP46313-100UG | Aviva Systems Biology | 100ug | EUR 99 |
AAP46314-100UG - MCCC2 Peptide - N-terminal region |
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AAP46314-100UG | Aviva Systems Biology | 100ug | EUR 99 |
AAP82826-100UG - MCCC2 Peptide - C-terminal region |
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AAP82826-100UG | Aviva Systems Biology | 100ug | EUR 99 |
Mccc2 (Myc-DDK-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein |
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MR224804 | Origene Technologies GmbH | 10 µg | Ask for price |
Mccc2 (untagged ORF) - Rat methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10 ug) |
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RN203166 | Origene Technologies GmbH | 10 µg | Ask for price |
MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV655585 | ABM | 1.0 ug DNA | EUR 818.4 |
MCCC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV655589 | ABM | 1.0 ug DNA | EUR 818.4 |
MCCC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV655590 | ABM | 1.0 ug DNA | EUR 818.4 |
The tactic provides the benefit of being carried out on stay cells, assuaging the necessity for a recombinant supply of the receptor. Moreover, time-resolved measurements, along with permitting the willpower of the affinity of the studied drug to its goal, give entry to the binding kinetics thereby offering a full characterization of the system. On this research, we additionally in contrast time-resolved LigandTracer information with end-point OkayD willpower from circulation cytometry experiments and hypothesize that discrepancies between these two approaches, once they exist, typically come from circulation cytometry titration curves being acquired previous to full equilibration of the system.