Highly sensitive detection of antibody nonspecific interactions using flow cytometry

The quickly evolving nature of antibody drug improvement has resulted in applied sciences that generate huge numbers (a whole bunch to hundreds) of lead antibody candidates throughout early discovery. These candidates have to be quickly pared all the way down to establish probably the most drug-like candidates for in-depth evaluation of their security and efficacy, which may solely be carried out on a restricted variety of antibodies attributable to time and useful resource necessities. One key biophysical property of profitable antibody therapeutics is excessive specificity, outlined as low ranges of nonspecific binding or polyspecificity.

Though there was some progress in creating assays for detecting antibody polyspecificity, most of those assays are restricted by poor sensitivity or assay codecs that require proprietary antibody floor show strategies, and a few of these assays use advanced and poorly outlined polyspecificity reagents. Right here we report the PolySpecificity Particle (PSP) assay, a delicate circulation cytometry assay for evaluating antibody nonspecific interactions that overcomes earlier limitations and can be utilized for evaluating various sorts of IgGs, multispecific antibodies and Fc-fusion proteins. Our strategy makes use of micron-sized magnetic beads coated with Protein A to seize antibodies at extraordinarily dilute concentrations (<0.02 mg/mL).

Move cytometry evaluation of polyspecificity reagent binding to those conjugates ends in delicate detection of variations in nonspecific interactions for clinical-stage antibodies. Our PSP assay strongly discriminates between antibodies with totally different ranges of polyspecificity utilizing beforehand reported polyspecificity reagents which are both well-defined proteins or extremely advanced protein mixtures. Furthermore, we additionally discover {that a} distinctive reagent, particularly ovalbumin, ends in the very best assay sensitivity and specificity. Importantly, our assay is far more delicate than customary assays equivalent to ELISAs. We anticipate that our easy, delicate, and high-throughput PSP assay will speed up the event of protected and efficient antibody therapeutics.

Toxicity of Carbon Nanomaterials-In the direction of Dependable Viability Evaluation by way of New Method in Move Cytometry

The scope of software of carbon nanomaterials in biomedical, environmental and industrial fields is lately considerably growing. Since in vitro toxicity testing is the primary important step for any business utilization, it’s essential to have a dependable methodology to investigate the possibly dangerous results of carbon nanomaterials. Regardless that researchers already reported the interference of carbon nanomaterials with widespread toxicity assays, there’s nonetheless, sadly, a lot of research that neglect this reality. On this research, we investigated interference of 4 bio-promising carbon nanomaterials (graphene acid (GA), cyanographene (GCN), graphitic carbon nitride (g-C3N4) and carbon dots (QCDs)) in generally used LIVE/DEAD assay.

When a regular process was utilized, supplies prompted varied sorts of interference. Whereas positively charged g-C3N4 and QCDs induced false outcomes by means of the creation of free agglomerates and intrinsic fluorescence properties, negatively charged GA and GCN led to false indicators as a result of advanced quenching impact of the fluorescent dye of a LIVE/DEAD package. Thus, we developed a brand new strategy utilizing a particular gating technique primarily based on extra controls that efficiently overcame all sorts of interference and result in dependable ends in LIVE/DEAD assay.

We advise that the newly developed process ought to be a compulsory device for all in vitro circulation cytometry assays of any class of carbon nanomaterials. Imaging circulation cytometry permits for the quantitative evaluation of fluorescent indicators on the subcellular degree. Right here, we describe using a biosensor cell line, particularly, U2OS osteosarcoma cells outfitted with a fusion protein comprising monomeric pink fluorescent protein (mRFP), inexperienced fluorescent protein (GFP) and microtubule-associated proteins 1A/1B gentle chain 3B (greatest often called LC3), for the evaluation of autophagic flux by imaging circulation cytometry.

Highly sensitive detection of antibody nonspecific interactions using flow cytometry

We element all evaluation instruments required to tell apart autophagosomes (that emit each a pink and a inexperienced fluorescence) and autolysosomes (that emit a pink fluorescence, but lose the inexperienced fluorescent sign) and to quantitate autophagic flux in a handy style. Our information, nevertheless, present that information of the kinetics of the interplay permits to reconcile the info obtained by circulation cytometry and LigandTracer and show the complementarity of those two strategies.

Okay D willpower from time-resolved experiments on stay cells with LigandTracer and reconciliation with end-point circulation cytometry measurements

Design of next-generation therapeutics comes with new challenges and emulates expertise and strategies to satisfy them. Characterizing the binding of both pure ligands or therapeutic proteins to cell-surface receptors, for which related recombinant variations could not exist, represents certainly one of these challenges. Right here we report the characterization of the interplay of 5 totally different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate goal receptors utilizing LigandTracer.

MCCC2 Antibody

DF12655-100ul 100ul
EUR 280

MCCC2 Antibody

DF12655-200ul 200ul
EUR 350

MCCC2 antibody

E39-05048 100ug/100ul
EUR 225
Description: Available in various conjugation types.

MCCC2 Antibody

E306903 100ug/200ul
EUR 275
Description: Available in various conjugation types.

MCCC2 antibody

70R-18436 50 ul
EUR 289
Description: Rabbit polyclonal MCCC2 antibody

MCCC2 Antibody

1-CSB-PA867199ESR1HU
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  • 100ul
  • 50ul
Description: A polyclonal antibody against MCCC2. Recognizes MCCC2 from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

MCCC2 Polyclonal Antibody

28858 100ul
EUR 439

MCCC2 Polyclonal Antibody

28858-100ul 100ul
EUR 302.4

MCCC2 Polyclonal Antibody

28858-50ul 50ul
EUR 224.4

MCCC2 Polyclonal Antibody

31405 100ul
EUR 439

MCCC2 Polyclonal Antibody

31405-100ul 100ul
EUR 302.4

MCCC2 Polyclonal Antibody

31405-50ul 50ul
EUR 224.4

MCCC2 Polyclonal Antibody

E915181 100ul
EUR 225
Description: Available in various conjugation types.

MCCC2 Polyclonal Antibody

E-AB-66453-120uL 120uL
EUR 320
Description: Unconjugated

MCCC2 Polyclonal Antibody

E-AB-66453-200uL 200uL
EUR 530
Description: Unconjugated

MCCC2 Polyclonal Antibody

E-AB-66453-60uL 60uL
EUR 200
Description: Unconjugated

MCCC2 Polyclonal Antibody

E-AB-66453-each each Ask for price
Description: Unconjugated

OACD00477-1ML - MCCC2 Antibody

OACD00477-1ML 1ml
EUR 739

OACD00478-1ML - MCCC2 Antibody

OACD00478-1ML 1ml
EUR 649

OACA08635-50UL - MCCC2 Antibody

OACA08635-50UL 50ul
EUR 189

OAAN02555-50UL - MCCC2 Antibody

OAAN02555-50UL 50ul
EUR 149

Polyclonal MCCC2 Antibody (Center)

APR10893G 0.1ml
EUR 633.6
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human MCCC2 (Center). This antibody is tested and proven to work in the following applications:

OACA08635-100UL - MCCC2 Antibody

OACA08635-100UL 100ul
EUR 230

OAGA01973-100UL - MCCC2 Antibody

OAGA01973-100UL 100ul
EUR 369

OAGA05503-100UL - MCCC2 Antibody

OAGA05503-100UL 100ul
EUR 369

OAAN02555-100UL - MCCC2 Antibody

OAAN02555-100UL 100ul
EUR 249

OAAN02555-200UL - MCCC2 Antibody

OAAN02555-200UL 200ul
EUR 389

OAAL00786-100UG - MCCC2 Antibody

OAAL00786-100UG 100ug
EUR 349

OACD00477-100UL - MCCC2 Antibody

OACD00477-100UL 100ul
EUR 209

OACD00478-100UL - MCCC2 Antibody

OACD00478-100UL 100ul
EUR 199

[One Step] MCCC2 Antibody Kit

RK05665 50 ul
EUR 288

MCCC2 Polyclonal Conjugated Antibody

C31405 100ul
EUR 476.4

MCCC2 Polyclonal Conjugated Antibody

C28858 100ul
EUR 476.4

MCCC2 (Center) rabbit polyclonal antibody

AP17553PU-N 400 µl Ask for price

OAAB04039-400UL - MCCC2 Antibody - middle region

OAAB04039-400UL 400ul
EUR 409

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

20-abx322517
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  • 100 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx037012-100ug 100 ug
EUR 469.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx235048-100ug 100 ug
EUR 610.8

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

20-abx006595
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  • 100 ul
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  • 20 ul
  • 50 ul

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx030686-400ul 400 ul
EUR 627.6

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx030686-80l 80 µl
EUR 343.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx033068-400ul 400 ul
EUR 627.6

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx033068-80l 80 µl
EUR 343.2

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx006595-100g 100 µg
EUR 275

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx006595-10g 10 µg
EUR 175

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx006595-200g 200 µg
EUR 400

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx235048-100g 100 µg
EUR 350

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx037012-1096tests 10 × 96 tests Ask for price

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx037012-596tests 5 × 96 tests Ask for price

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx037012-96tests 96 tests
EUR 337.5

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx030686-400l 400 µl
EUR 518.75

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx033068-400l 400 µl
EUR 518.75

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx322517-100g 100 µg
EUR 350

Methylcrotonoyl-CoA Carboxylase 2 (MCCC2) Antibody

abx322517-50g 50 µg
EUR 250

ARP82826_P050-25UL - MCCC2 Antibody - C-terminal region

ARP82826_P050-25UL 25ul
EUR 99

ARP46313_P050-25UL - MCCC2 Antibody - C-terminal region

ARP46313_P050-25UL 25ul
EUR 99

ARP46314_P050-25UL - MCCC2 Antibody - N-terminal region

ARP46314_P050-25UL 25ul
EUR 99

OAAB14516-400UL - MCCC2 Antibody - C-terminal region

OAAB14516-400UL 400ul
EUR 389

ARP82826_P050 - MCCC2 Antibody - C-terminal region (ARP82826_P050)

ARP82826_P050 100ul
EUR 389

ARP46313_P050 - MCCC2 Antibody - C-terminal region (ARP46313_P050)

ARP46313_P050 100ul
EUR 389

ARP46314_P050 - MCCC2 Antibody - N-terminal region (ARP46314_P050)

ARP46314_P050 100ul
EUR 389

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx131298
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx131299
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

20-abx113712
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  • 150 ul
  • 50 ul

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

abx113712-100l 100 µl
EUR 612.5

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

abx131298-05mg 0.5 mg
EUR 275

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Antibody

abx131299-05mg 0.5 mg
EUR 287.5

Mccc2/ Rat Mccc2 ELISA Kit

ELI-48233r 96 Tests
EUR 1063.2

MCCC2

E8ER1912-86 100ul
EUR 275
Description: Available in various conjugation types.

MCCC2

CSB-CL867199HU1 10 μg plasmid + 200μl Glycerol Ask for price

MCCC2

CSB-CL867199HU2 10 μg plasmid + 200μl Glycerol Ask for price

MCCC2 siRNA

20-abx923707
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  • 15 nmol
  • 30 nmol

MCCC2 siRNA

20-abx923708
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  • 15 nmol
  • 30 nmol

MCCC2 siRNA

20-abx903187
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  • 15 nmol
  • 30 nmol

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody

CAU23159-100ul 100ul
EUR 241.9

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody

CAU23159-200ul 200ul
EUR 302.4

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody

CAU23160-100ul 100ul
EUR 258.2

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody

CAU23160-200ul 200ul
EUR 322.6

Polyclonal Antibody to Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

PAD690Hu01 100ul
EUR 269

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human)

4-PAD690Hu01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse)

4-PAD690Mu01
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2)

MCCC2 Rabbit pAb

E2507990 100ul
EUR 225
Description: Available in various conjugation types.

MCCC2 Rabbit pAb

E2515181 100ul
EUR 225
Description: Available in various conjugation types.

MCCC2 Rabbit pAb

A15181-100ul 100 ul
EUR 369.6

MCCC2 Rabbit pAb

A15181-200ul 200 ul
EUR 550.8

MCCC2 Rabbit pAb

A15181-20ul 20 ul
EUR 219.6

MCCC2 Rabbit pAb

A15181-50ul 50 ul
EUR 267.6

MCCC2 Rabbit pAb

A7990-100ul 100 ul
EUR 369.6

MCCC2 Rabbit pAb

A7990-200ul 200 ul
EUR 550.8

MCCC2 Rabbit pAb

A7990-20ul 20 ul
EUR 219.6

MCCC2 Rabbit pAb

A7990-50ul 50 ul
EUR 267.6

MCCC2 cloning plasmid

CSB-CL867199HU1-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the MCCC2 gene.

MCCC2 cloning plasmid

CSB-CL867199HU2-10ug 10ug
EUR 451.2
Description: A cloning plasmid for the MCCC2 gene.

MCCC2 Blocking Peptide

DF12655-BP 1mg
EUR 234

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC

4-PAD690Hu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Cy3

4-PAD690Hu01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), HRP

4-PAD690Hu01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), PE

4-PAD690Hu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC

4-PAD690Mu01-APC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Cy3

4-PAD690Mu01-Cy3
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Cy3.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), HRP

4-PAD690Mu01-HRP
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with HRP.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), PE

4-PAD690Mu01-PE
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with PE.

anti- MCCC1 antibody

FNab05047 100µg
EUR 658.5
Description: Antibody raised against MCCC1

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), FITC

4-PAD690Hu01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), FITC

4-PAD690Mu01-FITC
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with FITC.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), Biotinylated

4-PAD690Hu01-Biotin
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), Biotinylated

4-PAD690Mu01-Biotin
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with Biotin.

Mouse Mccc2 ELISA KIT

ELI-46423m 96 Tests
EUR 1038

MCCC2 ELISA KIT|Human

EF000692 96 Tests
EUR 826.8

Human MCCC2 ELISA KIT

ELI-15862h 96 Tests
EUR 988.8

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Human), APC-Cy7

4-PAD690Hu01-APC-Cy7
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7.

Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2) Polyclonal Antibody (Mouse), APC-Cy7

4-PAD690Mu01-APC-Cy7
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Mouse Methylcrotonoyl Coenzyme A Carboxylase 2 (MCCC2). This antibody is labeled with APC-Cy7.

MCCC2 (Center) Rabbit pAb

E2620575 100ul
EUR 225
Description: Available in various conjugation types.

Rat MCCC2 shRNA Plasmid

20-abx990287
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  • 150 µg
  • 300 µg

Human MCCC2 shRNA Plasmid

20-abx961908
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  • 150 µg
  • 300 µg

Mouse MCCC2 shRNA Plasmid

20-abx978856
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  • 150 µg
  • 300 µg

MCCC2 Recombinant Protein (Rat)

RP211097 100 ug Ask for price

MCCC2 Recombinant Protein (Mouse)

RP149789 100 ug Ask for price

MCCC2 Recombinant Protein (Human)

RP018958 100 ug Ask for price

MCCC2 Recombinant Protein (Human)

RP018961 100 ug Ask for price

Mccc2 ORF Vector (Rat) (pORF)

ORF070367 1.0 ug DNA
EUR 607.2

MCCC2 ORF Vector (Human) (pORF)

ORF006320 1.0 ug DNA
EUR 114

MCCC2 ORF Vector (Human) (pORF)

ORF006321 1.0 ug DNA
EUR 114

Mccc2 ORF Vector (Mouse) (pORF)

ORF049931 1.0 ug DNA
EUR 607.2

Human MCCC2 knockout cell line

ABC-KH9104 1 vial Ask for price
Description: Human MCCC2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human MCCC2 knockdown cell line

ABC-KD9104 1 vial Ask for price
Description: Human MCCC2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human MCCC2 Protein Lysate 20ug

IHUMCCC2PLLY20UG each
EUR 213
Description: Human MCCC2 Protein Lysate 20ug

MCCC2 ELISA Kit (Human) (OKCD00916)

OKCD00916 96 Wells
EUR 997.2
Description: Description of target: Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.1 Publication <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.9"Expression, purification, characterization of human 3-methylcrotonyl-CoA carboxylase (MCCC)."_x005F_x005F_x000D_Chu C.H., Cheng D._x005F_x005F_x000D_Protein Expr. Purif. 53:421-427(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.141 ng/mL

OCOA08656-20UG - MCCC2 Protein Lysate

OCOA08656-20UG 20ug
EUR 169

MCCC2 Over-expression Lysate Product

GWB-C1A7F5 0.1 mg Ask for price

OPCD00165-10UG - MCCC2 Recombinant Protein

OPCD00165-10UG 10ug
EUR 149

OPCD00165-50UG - MCCC2 Recombinant Protein

OPCD00165-50UG 50ug
EUR 369

OPCD00166-10UG - MCCC2 Recombinant Protein

OPCD00166-10UG 10ug
EUR 159

OPCD00166-50UG - MCCC2 Recombinant Protein

OPCD00166-50UG 50ug
EUR 389

OPCD00165-200UG - MCCC2 Recombinant Protein

OPCD00165-200UG 200ug
EUR 739

OPCD00166-200UG - MCCC2 Recombinant Protein

OPCD00166-200UG 200ug
EUR 769

MCCC2 (untagged)-Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein

SC125472 10 µg Ask for price

OKCD00916-96W - MCCC2 ELISA Kit (Human)

OKCD00916-96W 96Wells
EUR 675

Mccc2 sgRNA CRISPR Lentivector set (Rat)

K7573601 3 x 1.0 ug
EUR 406.8

MCCC2 3'UTR GFP Stable Cell Line

TU063103 1.0 ml
EUR 1825.2

Mccc2 3'UTR GFP Stable Cell Line

TU162987 1.0 ml Ask for price

Mccc2 3'UTR GFP Stable Cell Line

TU262953 1.0 ml Ask for price

MCCC2 sgRNA CRISPR Lentivector set (Human)

K1278301 3 x 1.0 ug
EUR 406.8

Mccc2 sgRNA CRISPR Lentivector set (Mouse)

K3464301 3 x 1.0 ug
EUR 406.8

MCCC2 (GFP-tagged) - Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein

RG209488 10 µg Ask for price

MCCC2 Protein Vector (Rat) (pPM-C-HA)

PV281468 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPB-C-His)

PV281466 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPB-N-His)

PV281467 500 ng
EUR 723.6

MCCC2 Protein Vector (Rat) (pPM-C-His)

PV281469 500 ng
EUR 723.6

MCCC2 Protein Vector (Human) (pPM-C-HA)

PV025279 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-HA)

PV025283 500 ng
EUR 394.8

MCCC2 Protein Vector (Mouse) (pPM-C-HA)

PV199724 500 ng
EUR 723.6

MCCC2 Protein Vector (Human) (pPB-C-His)

PV025277 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-N-His)

PV025278 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-His)

PV025280 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-C-His)

PV025281 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPB-N-His)

PV025282 500 ng
EUR 394.8

MCCC2 Protein Vector (Human) (pPM-C-His)

PV025284 500 ng
EUR 394.8

MCCC2 Protein Vector (Mouse) (pPB-C-His)

PV199722 500 ng
EUR 723.6

MCCC2 Protein Vector (Mouse) (pPB-N-His)

PV199723 500 ng
EUR 723.6

MCCC2 Protein Vector (Mouse) (pPM-C-His)

PV199725 500 ng
EUR 723.6

Mccc2 3'UTR Luciferase Stable Cell Line

TU112987 1.0 ml Ask for price

MCCC2 3'UTR Luciferase Stable Cell Line

TU013103 1.0 ml
EUR 1825.2

Mccc2 3'UTR Luciferase Stable Cell Line

TU212953 1.0 ml Ask for price

MCCC2 (Myc-DDK-tagged)-Human methylcrotonoyl-CoA carboxylase 2 (beta) (MCCC2), nuclear gene encoding mitochondrial protein

RC209488 10 µg Ask for price

Mccc2 (untagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10ug)

MC219103 10 µg Ask for price

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7573602 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7573603 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7573604 1.0 ug DNA
EUR 184.8

Human MCCC2 Over-expressing Stable Cell Line

ABC-X1839 1 vial Ask for price
Description: Gentaur can provide custom lentiviral constructs expressing any genes of interest as long as it is less than about 3 kb. Lentiviral technology enables us to efficiently generate stable expression lines which are then selected for moderate or high expressers, depending on the experimental requirements. If you are interested in specific lentiviral DNA constructs or have further questions, please contact us to discuss the details. MCCC2 methylcrotonoyl-CoA carboxylase 2 (beta) [ Homo sapiens ] http://www.ncbi.nlm.nih.gov/gene/64087

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 1)

K1278302 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 2)

K1278303 1.0 ug DNA
EUR 184.8

MCCC2 sgRNA CRISPR Lentivector (Human) (Target 3)

K1278304 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3464302 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3464303 1.0 ug DNA
EUR 184.8

Mccc2 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3464304 1.0 ug DNA
EUR 184.8

MCCC1 Antibody

47317 100ul
EUR 319

MCCC1 Antibody

47317-100ul 100ul
EUR 302.4

MCCC1 Antibody

1-CSB-PA013572GA01HU
  • Ask for price
  • Ask for price
  • 150ul
  • 50ul
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

MCCC1 Antibody

E047317 100μg/100μl
EUR 255
Description: Available in various conjugation types.

MCCC1 Antibody

E306902 100ug/200ul
EUR 275
Description: Available in various conjugation types.

MCCC1 antibody

70R-18435 50 ul
EUR 289
Description: Rabbit polyclonal MCCC1 antibody

MCCC1 Antibody

1-CSB-PA853497EA01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IF; Recommended dilution: IF:1:50-1:200

MCCC1 Antibody

1-CSB-PA853497ESR1HU
  • Ask for price
  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC, IP; Recommended dilution: IHC:1:20-1:200, IP:1:200-1:2000

MCCC1 Antibody

1-CSB-PA853497ESR2HU
  • Ask for price
  • Ask for price
  • 100ul
  • 50ul
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200

Mccc2 (GFP-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2) nuclear gene encoding mitochondrial protein, (10ug)

MG224804 10 µg Ask for price

AAP46313-100UG - MCCC2 Peptide - C-terminal region

AAP46313-100UG 100ug
EUR 99

AAP46314-100UG - MCCC2 Peptide - N-terminal region

AAP46314-100UG 100ug
EUR 99

AAP82826-100UG - MCCC2 Peptide - C-terminal region

AAP82826-100UG 100ug
EUR 99

Mccc2 (Myc-DDK-tagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein

MR224804 10 µg Ask for price

Mccc2 (untagged ORF) - Rat methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10 ug)

RN203166 10 µg Ask for price

MCCC2 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV655585 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV655589 1.0 ug DNA
EUR 818.4

MCCC2 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV655590 1.0 ug DNA
EUR 818.4

The tactic provides the benefit of being carried out on stay cells, assuaging the necessity for a recombinant supply of the receptor. Moreover, time-resolved measurements, along with permitting the willpower of the affinity of the studied drug to its goal, give entry to the binding kinetics thereby offering a full characterization of the system. On this research, we additionally in contrast time-resolved LigandTracer information with end-point OkayD willpower from circulation cytometry experiments and hypothesize that discrepancies between these two approaches, once they exist, typically come from circulation cytometry titration curves being acquired previous to full equilibration of the system.

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