The mix of those ideas raised new analytical potentialities, opening an unprecedented subject of refined exploration for a lot of situations, together with hematopoiesis and hematological issues. On this assessment, the final ideas and progress achieved within the growth of recent analytical approaches for exploring high-dimensional knowledge units on the single-cell stage will likely be described as they appeared over the previous few years. A bigger and extra sensible half will element the assorted steps that should be mastered, each in knowledge acquisition and within the preanalytical test of information recordsdata.
Lastly, a step-by-step rationalization of the answer in growth to mix the Bioconductor clustering algorithm FlowSOM and the favored and broadly used software program Kaluza® (Beckman Coulter) will likely be introduced. The goal of this assessment was to level out that the day when these progresses will attain routine hematology laboratories doesn’t appear so far-off.
Detection of Immune Checkpoint Receptors – A Present Problem in Scientific Movement Cytometry
Immunological remedy ideas are more and more figuring out fashionable drugs. They’re used to deal with ailments of the immune system, for tumors, but additionally for infections, neurological ailments, and plenty of others. Most of those therapies base on antibodies, however small molecules, soluble receptors or cells and modified cells are additionally used. The event of immune checkpoint inhibitors is amazingly quick. T-cell directed antibody therapies in opposition to PD-1 or CTLA-Four are already firmly established within the clinic. Additional targets are continually being added and it’s changing into more and more clear that their expression just isn’t solely related on T cells. Moreover, we don’t but have any expertise with the long-term systemic results of the therapy.
Movement cytometry can be utilized for prognosis, monitoring, and detection of unwanted effects. On this assessment, we concentrate on checkpoint molecules as goal molecules and purposeful markers of cells of the innate and purchased immune system. Nonetheless, for many of the attention-grabbing and doubtlessly related parameters, there are nonetheless no take a look at kits appropriate for routine use. Right here we give an outline of the detection of checkpoint molecules on immune cells within the peripheral blood and present examples of a potential design of antibody panels.
The design of focused nanomedicines requires intracellular space- and time-resolved knowledge of nanoparticle distribution following uptake. Present strategies to review intracellular trafficking, akin to dynamic colocalization by fluorescence microscopy in stay cells, are normally low throughput and require in depth evaluation of enormous datasets to quantify colocalization in a number of particular person cells. Right here a way primarily based on move cytometry to simply detect and characterize the organelles through which nanoparticles are internalized and trafficked over time is proposed.
Standard cell fractionation strategies are mixed with immunostaining and high-sensitivity organelle move cytometry to get space-resolved knowledge of nanoparticle intracellular distribution. By extracting the organelles at completely different instances, time-resolved knowledge of nanoparticle intracellular trafficking are obtained. The strategy is validated by figuring out how nanoparticle measurement impacts the kinetics of arrival to the lysosomes. The outcomes reveal that this methodology permits high-throughput evaluation of nanoparticle uptake and intracellular trafficking by cells, subsequently it may be used to find out how nanoparticle design impacts their intracellular habits.
Movement cytometry knowledge mining by cytoChain identifies determinants of exhaustion and stemness in TCR-engineered T cells
The phenotype of infused cells is a serious determinant of Adoptive T-cell remedy (ACT) efficacy. But, the problem in deciphering multiparametric cytometry knowledge restricted the high quality characterization of mobile merchandise. To permit the evaluation of dynamic and sophisticated move cytometry samples we developed cytoChain, a novel dataset mining instrument and a brand new analytical workflow. CytoChain was challenged to match state-of-the-art and revolutionary tradition situations to generate stem-like reminiscence cells (TSCM ) appropriate for ACT. Noticeably, the mix of IL-7/15 and super-oxides scavenging sustained the emergence of a beforehand un-identified non-exhausted Match-TSCM signature, ignored by handbook gating and endowed with superior growth potential.
CytoChain proficiently traced again this inhabitants in unbiased datasets, and in T-cell receptor engineered lymphocytes. cytoChain flexibility and performance had been then additional validated on a broadcast dataset from circulating T cells in COVID-19 sufferers. Collectively, our outcomes assist using cytoChain to establish novel, functionally essential immunophenotypes for ACT and sufferers immunomonitoring. This text is protected by copyright. All rights reserved. Environment friendly doubled haploid (DH) plant manufacturing is of nice curiosity within the plant breeding business and analysis as a result of homozygous traces are obtained inside a single era shortening the breeding cycle considerably.
SDCCAG8 antibody |
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| 70R-20131 | Fitzgerald | 50 ul | EUR 435 |
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Description: Rabbit polyclonal SDCCAG8 antibody |
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SDCCAG8 antibody |
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| 70R-4002 | Fitzgerald | 50 ug | EUR 467 |
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Description: Rabbit polyclonal SDCCAG8 antibody raised against the N terminal of SDCCAG8 |
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SDCCAG8 antibody |
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| 70R-4128 | Fitzgerald | 50 ug | EUR 467 |
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Description: Rabbit polyclonal SDCCAG8 antibody raised against the middle region of SDCCAG8 |
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Mouse Monoclonal Anti-Actin (Pan, alpha, beta, gamma) IgG-Cy3 conjugate |
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| ACTB24-Cy3 | Alpha Diagnostics | 100 ul | EUR 408 |
Goat Anti-Mouse IgG-Cy3 Conjugate (Adsorbed with Bov, Ch, Gt, Gp, Ha, Hs, Hu, Rb, Sh) |
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| 40337-Cy3 | Alpha Diagnostics | 0.5 ml | EUR 263 |
Anti-SDCCAG8 antibody |
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| STJ72779 | St John's Laboratory | 100 µg | EUR 359 |
anti- SDCCAG8 antibody |
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| FNab07666 | FN Test | 100µg | EUR 505.25 |
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Description: Antibody raised against SDCCAG8 |
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Anti-SDCCAG8 antibody |
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| PAab07666 | Lifescience Market | 100 ug | EUR 355 |
SDCCAG8 siRNA |
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| 20-abx932704 | Abbexa |
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SDCCAG8 siRNA |
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| 20-abx932705 | Abbexa |
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anti-SDCCAG8 |
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| YF-PA17224 | Abfrontier | 50 ul | EUR 363 |
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Description: Mouse polyclonal to SDCCAG8 |
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anti-SDCCAG8 |
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| YF-PA17225 | Abfrontier | 50 ug | EUR 363 |
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Description: Mouse polyclonal to SDCCAG8 |
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SDCCAG8 Peptide |
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| 43-022P | ProSci | 0.1 mg | EUR 338 |
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Description: SDCCAG8 Peptide |
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SDCCAG8 Peptide |
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| 43-023P | ProSci | 0.1 mg | EUR 338 |
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Description: SDCCAG8 Peptide |
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Polyclonal SDCCAG8 Antibody (Internal) |
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| APG01216G | Leading Biology | 0.05mg | EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human SDCCAG8 (Internal). This antibody is tested and proven to work in the following applications: |
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SDCCAG8 Blocking Peptide |
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| 20-abx161437 | Abbexa |
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SDCCAG8 cloning plasmid |
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| CSB-CL020899HU-10ug | Cusabio | 10ug | EUR 413 |
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Description: A cloning plasmid for the SDCCAG8 gene. |
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SDCCAG8 Blocking Peptide |
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| DF8846-BP | Affbiotech | 1mg | EUR 195 |
SDCCAG8 Blocking Peptide |
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| 33R-3716 | Fitzgerald | 100 ug | EUR 180 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of SDCCAG8 antibody, catalog no. 70R-4002 |
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SDCCAG8 Blocking Peptide |
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| 33R-4109 | Fitzgerald | 100 ug | EUR 180 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of SDCCAG8 antibody, catalog no. 70R-4128 |
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Anti-SDCCAG8 (aa469-483) antibody |
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| STJ72780 | St John's Laboratory | 100 µg | EUR 359 |
Polyclonal SDCCAG8 Antibody (internal region) |
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| APG00810G | Leading Biology | 0.1mg | EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human SDCCAG8 (internal region). This antibody is tested and proven to work in the following applications: |
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Polyclonal SDCCAG8 Antibody (aa469-483) |
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| APG01240G | Leading Biology | 0.05mg | EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human SDCCAG8 (aa469-483). This antibody is tested and proven to work in the following applications: |
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Cy3 Conjugated Avidin |
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| BA1037 | BosterBio | 0.5ml | EUR 162 |
Mouse SDCCAG8 shRNA Plasmid |
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| 20-abx978693 | Abbexa |
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Human SDCCAG8 shRNA Plasmid |
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| 20-abx957365 | Abbexa |
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SDCCAG8 ELISA KIT|Human |
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| EF002773 | Lifescience Market | 96 Tests | EUR 689 |
Human SDCCAG8 ELISA KIT |
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| ELI-29645h | Lifescience Market | 96 Tests | EUR 824 |
Mouse Sdccag8 ELISA KIT |
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| ELI-13424m | Lifescience Market | 96 Tests | EUR 865 |
SDCCAG8 Recombinant Protein (Mouse) |
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| RP170432 | ABM | 100 ug | Ask for price |
SDCCAG8 Recombinant Protein (Human) |
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| RP096258 | ABM | 100 ug | Ask for price |
pCMV-SPORT6-SDCCAG8 Plasmid |
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| PVT16613 | Lifescience Market | 2 ug | EUR 325 |
SDCCAG8 Recombinant Protein (Rat) |
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| RP227798 | ABM | 100 ug | Ask for price |
Polyclonal SDCCAG8 (aa469-483) Antibody (internal region) |
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| APG00811G | Leading Biology | 0.1mg | EUR 484 |
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Description: A polyclonal antibody raised in Goat that recognizes and binds to Human SDCCAG8 (aa469-483) (internal region). This antibody is tested and proven to work in the following applications: |
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Sdccag8 ORF Vector (Mouse) (pORF) |
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| ORF056812 | ABM | 1.0 ug DNA | EUR 506 |
SDCCAG8 ORF Vector (Human) (pORF) |
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| ORF032087 | ABM | 1.0 ug DNA | EUR 405 |
Sdccag8 ORF Vector (Rat) (pORF) |
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| ORF075934 | ABM | 1.0 ug DNA | EUR 506 |
Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| 20-abx121727 | Abbexa |
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Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| 20-abx115458 | Abbexa |
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Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| abx029545-400ul | Abbexa | 400 ul | EUR 523 |
Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| abx029545-80l | Abbexa | 80 µl | EUR 286 |
Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| abx432203-200ul | Abbexa | 200 ul | EUR 384 |
Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| abx432204-200ul | Abbexa | 200 ul | EUR 286 |
Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| abx237666-100ug | Abbexa | 100 ug | EUR 481 |
Serologically Defined Colon Cancer Antigen 8 (SDCCAG8) Antibody |
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| 20-abx325189 | Abbexa |
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Sdccag8 sgRNA CRISPR Lentivector set (Mouse) |
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| K3434501 | ABM | 3 x 1.0 ug | EUR 339 |
SDCCAG8 sgRNA CRISPR Lentivector set (Human) |
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| K2108401 | ABM | 3 x 1.0 ug | EUR 339 |
Sdccag8 sgRNA CRISPR Lentivector set (Rat) |
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| K7466701 | ABM | 3 x 1.0 ug | EUR 339 |
Goat Anti-Rabbit IgG Secondary Antibody Cy3 Conjugated |
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| L30111 | SAB | 1.0 ml | EUR 112 |
Goat Anti-Human IgG Secondary Antibody Cy3 Conjugated |
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| L30211 | SAB | 1.0 ml | EUR 112 |
Goat Anti-Mouse IgG Secondary Antibody Cy3 Conjugated |
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| L30311 | SAB | 1.0 ml | EUR 112 |
Sdccag8 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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| K3434502 | ABM | 1.0 ug DNA | EUR 154 |
Sdccag8 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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| K3434503 | ABM | 1.0 ug DNA | EUR 154 |
Sdccag8 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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| K3434504 | ABM | 1.0 ug DNA | EUR 154 |
SDCCAG8 sgRNA CRISPR Lentivector (Human) (Target 1) |
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| K2108402 | ABM | 1.0 ug DNA | EUR 154 |
SDCCAG8 sgRNA CRISPR Lentivector (Human) (Target 2) |
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| K2108403 | ABM | 1.0 ug DNA | EUR 154 |
SDCCAG8 sgRNA CRISPR Lentivector (Human) (Target 3) |
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| K2108404 | ABM | 1.0 ug DNA | EUR 154 |
Sdccag8 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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| K7466702 | ABM | 1.0 ug DNA | EUR 154 |
Sdccag8 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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| K7466703 | ABM | 1.0 ug DNA | EUR 154 |
Sdccag8 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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| K7466704 | ABM | 1.0 ug DNA | EUR 154 |
Sdccag8 3'UTR Luciferase Stable Cell Line |
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| TU220027 | ABM | 1.0 ml | Ask for price |
Sdccag8 3'UTR GFP Stable Cell Line |
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| TU270027 | ABM | 1.0 ml | Ask for price |
Sdccag8 3'UTR GFP Stable Cell Line |
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| TU168462 | ABM | 1.0 ml | Ask for price |
Sdccag8 3'UTR Luciferase Stable Cell Line |
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| TU118462 | ABM | 1.0 ml | Ask for price |
SDCCAG8 3'UTR Luciferase Stable Cell Line |
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| TU022786 | ABM | 1.0 ml | EUR 1394 |
SDCCAG8 3'UTR GFP Stable Cell Line |
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| TU072786 | ABM | 1.0 ml | EUR 1394 |
SDCCAG8 Protein Vector (Mouse) (pPB-C-His) |
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| PV227246 | ABM | 500 ng | EUR 1065 |
SDCCAG8 Protein Vector (Mouse) (pPB-N-His) |
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| PV227247 | ABM | 500 ng | EUR 1065 |
SDCCAG8 Protein Vector (Mouse) (pPM-C-HA) |
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| PV227248 | ABM | 500 ng | EUR 1065 |
SDCCAG8 Protein Vector (Mouse) (pPM-C-His) |
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| PV227249 | ABM | 500 ng | EUR 1065 |
SDCCAG8 Protein Vector (Rat) (pPB-C-His) |
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| PV303734 | ABM | 500 ng | EUR 1166 |
SDCCAG8 Protein Vector (Rat) (pPB-N-His) |
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| PV303735 | ABM | 500 ng | EUR 1166 |
SDCCAG8 Protein Vector (Rat) (pPM-C-HA) |
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| PV303736 | ABM | 500 ng | EUR 1166 |
SDCCAG8 Protein Vector (Rat) (pPM-C-His) |
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| PV303737 | ABM | 500 ng | EUR 1166 |
SDCCAG8 Protein Vector (Human) (pPB-C-His) |
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| PV128346 | ABM | 500 ng | EUR 552 |
SDCCAG8 Protein Vector (Human) (pPB-N-His) |
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| PV128347 | ABM | 500 ng | EUR 552 |
SDCCAG8 Protein Vector (Human) (pPM-C-HA) |
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| PV128348 | ABM | 500 ng | EUR 552 |
SDCCAG8 Protein Vector (Human) (pPM-C-His) |
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| PV128349 | ABM | 500 ng | EUR 552 |
SDCCAG8 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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| LV637435 | ABM | 1.0 ug DNA | EUR 1355 |
SDCCAG8 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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| LV637439 | ABM | 1.0 ug DNA | EUR 1355 |
SDCCAG8 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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| LV637440 | ABM | 1.0 ug DNA | EUR 1355 |
Cy3 Conjugated anti-Mouse IgG SABC Kit |
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| SA1072 | BosterBio | 1 kit | EUR 355 |
Cy3 Conjugated anti-Mouse IgM SABC Kit |
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| SA1073 | BosterBio | 1 kit | EUR 375 |
Cy3 Conjugated anti-Rabbit IgG SABC Kit |
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| SA1074 | BosterBio | 1 kit | EUR 355 |
Cy3 Conjugated anti-Goat IgG SABC Kit |
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| SA1076 | BosterBio | 1 kit | EUR 355 |
Cy3 Conjugated anti-Human IgG SABC Kit |
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| SA1078 | BosterBio | 1 kit | EUR 355 |
Cy3 Conjugated anti-Rat IgG SABC Kit |
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| SA1079 | BosterBio | 1 kit | EUR 355 |
Cy3 Conjugated Goat Anti-Mouse IgG (H+L) secondary antibody |
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| BA1031 | BosterBio | 0.5ml | EUR 182 |
Cy3 Conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody |
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| BA1032 | BosterBio | 0.5ml | EUR 182 |
Cy3 Conjugated Rabbit Anti-Goat IgG (H+L) secondary antibody |
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| BA1034 | BosterBio | 0.5ml | EUR 182 |
Cy3 Conjugated Rabbit Anti-Human IgG (H+L) secondary antibody |
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| BA1035 | BosterBio | 0.5ml | EUR 182 |
Cy3 tertrazine [Cy3 tertrazine] |
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| 910 | AAT Bioquest | 1 mg | EUR 219 |
DH protocol growth is usually a time- and resource-consuming course of resulting from quite a few components affecting its success and effectivity. Right here we current ideas and examples about how essential success components might be recognized all through a DH protocol and an early microspore response monitored by easy impedance move cytometry (IFC) measurements, which can assist to optimize every step of an androgenesis-based DH protocol.