Plasma cell myeloma (a number of myeloma [MM]) is a malignant neoplasm originating from the plasma cells. Apart from different strategies, circulation cytometric evaluation of the affected person’s bone marrow aspirate has an vital function within the prognosis and in addition within the response evaluation. Because the cell floor markers, used for figuring out irregular plasma cells, are expressed diversely and the remedy also can alter the phenotype of the plasma cells, there may be an rising demand for brand spanking new plasma cell markers. VS38c is a monoclonal antibody that acknowledges the CLIMP-63 protein within the membrane of the endoplasmic reticulum.
CLIMP-63 is understood to be expressed at excessive ranges in regular and pathologic plasma cells within the bone marrow, thus VS38c antibody can be utilized to determine them. Though VS38c staining of plasma cells is reported to be fixed and robust even in myeloma, we have been questioning whether or not pattern preparation can have an effect on the staining. We’ve investigated the impact of various permeabilization brokers and washing of the cells on the standard of the VS38c staining and located that in lots of circumstances the staining is insufficient to determine the plasma cells.
We measured the VS38c staining of the bone marrow aspirates of 196 MM sufferers and noticed that the majority circumstances confirmed vivid staining with VS38c. Nonetheless, permeabilization with gentle detergent resulted within the look of a big VS38cdim subpopulation, which confirmed elevated sensitivity to mechanical stress (centrifugation). Our outcomes point out that VS38cdim MM cells can seem as a result of improper permeabilization of the endoplasmic reticulum and this discovering raises the opportunity of the existence of a plasma cell subpopulation with totally different membrane properties.
The importance of this inhabitants is unclear but, however these cells could be simply missed with VS38c staining and could be misplaced because of centrifugation-induced lysis throughout pattern preparation. Within the wholesome adults, the reference interval for the neutrophil phagocytosis to Escherichia coli was 46.91%-83.09% and to Staphylococcus aureus was 33.92%-69.48%. This methodology confirmed good reproducibility. Neutrophil phagocytosis was negatively correlated with the neutrophil depend, neutrophil share, and neutrophil-to-lymphocyte ratio
Quantification of Extracellular Double-stranded RNA Uptake and Subcellular Localization Utilizing Stream Cytometry and Confocal Microscopy
Double-stranded RNA is a potent pathogen-associated molecular sample (PAMP) produced as a by-product of viral replication and a well known hallmark of viral an infection. Viral dsRNAs could be launched from contaminated cells into the extracellular house and internalized by neighboring cells by way of endocytosis. Mammals possess a number of sample recognition receptors (PRRs) able to detecting viral dsRNAs reminiscent of endosomal toll-like receptor 3 (TLR3) and cytosolic RIG-I-like receptors (RLRs) which result in the manufacturing of kind I interferons (IFNs). Thus, intracellular localization of viral dsRNA can present perception into the downstream signaling pathways resulting in innate immune activation.
Right here, we describe a quantitative methodology for measuring extracellular dsRNA uptake and visualizing subcellular localization of internalized dsRNA by way of circulation cytometry and confocal microscopy respectively. The protocol described right here has been developed to detect RNA on the single cell degree. Fluorescent probes hybridize to focus on RNAs and are detected by circulation cytometry after a number of amplification steps. Several types of RNA could be detected reminiscent of mRNA, lengthy noncoding RNA, viral RNA or telomere RNA and as much as four totally different goal probes can be utilized concurrently. We used this protocol to particularly measure the expression of two transcription issue mRNAs, MAFB and IRF4, in human monocytes.
Analysis of measurable residual illness in a number of myeloma by multiparametric circulation cytometry: Present paradigm, pointers, and future purposes
A number of myeloma (MM) is a heterogeneous group of mature B-cell ailments which might be sometimes characterised by the presence and accumulation of irregular plasma cells (PCs), which ends up in the surplus manufacturing of monoclonal immunoglobulin and/or gentle chain discovered within the serum and/or urine. Multiparametric circulation cytometry (MFC) is an indispensable software to complement the prognosis, classification and monitoring of the illness because of its excessive affected person applicability, wonderful sensitivity and inspiring outcomes from numerous scientific trials.
On this regard, minimal or, extra appropriately, measurable residual illness (MRD) negativity by MFC has been acknowledged as a robust predictor of beneficial long-term outcomes. Earlier than circulation cytometry could be successfully carried out within the scientific setting for MM MRD testing, pattern preparation, panel configuration, evaluation and gating methods have to be optimized to make sure correct outcomes. This manuscript will talk about the present consensus pointers for circulation cytometric processing of samples and reporting of outcomes for MM MRD testing.
We additionally talk about various approaches to detect plasma cells within the presence of daratumumab remedy. Lastly, there’s a lack of awareness describing the subclonal distribution of myeloma cells based mostly on their protein expression. The arrival of high-dimensional evaluation might help in following the evolution of antigen expression patterns on irregular plasma cells in sufferers with relapsed/refractory illness. This in flip may also help determine clonal subtypes which might be extra aggressive for potential knowledgeable resolution.
MCCC1 Antibody |
|||
| 47317-100ul | SAB | 100ul | EUR 252 |
MCCC1 antibody |
|||
| 70R-18435 | Fitzgerald | 50 ul | EUR 435 |
|
Description: Rabbit polyclonal MCCC1 antibody |
|||
anti-MCCC1 |
|||
| YF-PA20071 | Abfrontier | 50 ug | EUR 363 |
|
Description: Mouse polyclonal to MCCC1 |
|||
HEK-293T Telomerase Over-Expressing Cell Pellet |
|||
| abx069991-1Pellet | Abbexa | 1 Pellet | EUR 398 |
MCCC1 cloning plasmid |
|||
| CSB-CL853497HU-10ug | Cusabio | 10ug | EUR 474 |
|
Description: A cloning plasmid for the MCCC1 gene. |
|||
Anti-MCCC1 antibody |
|||
| STJ112060 | St John's Laboratory | 100 µl | EUR 277 |
|
Description: This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. |
|||
MCCC1 Rabbit pAb |
|||
| A10020-100ul | Abclonal | 100 ul | EUR 308 |
MCCC1 Rabbit pAb |
|||
| A10020-200ul | Abclonal | 200 ul | EUR 459 |
MCCC1 Rabbit pAb |
|||
| A10020-20ul | Abclonal | 20 ul | EUR 183 |
MCCC1 Rabbit pAb |
|||
| A10020-50ul | Abclonal | 50 ul | EUR 223 |
anti- MCCC1 antibody |
|||
| FNab05047 | FN Test | 100µg | EUR 548.75 |
|
Description: Antibody raised against MCCC1 |
|||
MCCC1 Conjugated Antibody |
|||
| C47317 | SAB | 100ul | EUR 397 |
Anti-MCCC1 antibody |
|||
| PAab05047 | Lifescience Market | 100 ug | EUR 386 |
Anti-MCCC1 (2G8) |
|||
| YF-MA18990 | Abfrontier | 100 ug | EUR 363 |
|
Description: Mouse monoclonal to MCCC1 |
|||
Beaucage reagent |
|||
| HY-100951 | MedChemExpress | 10mM/1mL | EUR 126 |
BOP reagent |
|||
| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
|||
| 5-02142 | CHI Scientific | 100g | Ask for price |
Bradford reagent |
|||
| BDE641 | Bio Basic | 100ml | EUR 61.01 |
BOP reagent |
|||
| A7015-100000 | ApexBio | 100 g | EUR 200 |
|
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
|||
BOP reagent |
|||
| A7015-25000 | ApexBio | 25 g | EUR 113 |
|
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
|||
Bluing Reagent |
|||
| BRT030 | ScyTek Laboratories | 30 ml | EUR 60 |
Bluing Reagent |
|||
| BRT125 | ScyTek Laboratories | 125 ml | EUR 63 |
Bluing Reagent |
|||
| BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 184 |
Bluing Reagent |
|||
| BRT500 | ScyTek Laboratories | 500 ml | EUR 76 |
Bluing Reagent |
|||
| BRT999 | ScyTek Laboratories | 1000 ml | EUR 88 |
Chymase reagent |
|||
| 30C-CP1129 | Fitzgerald | 5 units | EUR 2185 |
|
Description: Purified native Human Chymase reagent |
|||
Traut's Reagent |
|||
| 2330-1000 | Biovision | EUR 349 | |
Traut's Reagent |
|||
| 2330-500 | Biovision | EUR 207 | |
MTS Reagent |
|||
| 2808-1000 | Biovision | EUR 990 | |
MTS Reagent |
|||
| 2808-250 | Biovision | EUR 365 | |
MTT Reagent |
|||
| 2809-1G | Biovision | EUR 180 | |
MTT Reagent |
|||
| 2809-5G | Biovision | EUR 544 | |
MCCC1 Antibody, HRP conjugated |
|||
| 1-CSB-PA853497EB01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
|||
MCCC1 Antibody, FITC conjugated |
|||
| 1-CSB-PA853497EC01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
|||
MCCC1 Antibody, Biotin conjugated |
|||
| 1-CSB-PA853497ED01HU | Cusabio |
|
|
|
Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
|||
Human MCCC1 shRNA Plasmid |
|||
| 20-abx961224 | Abbexa |
|
|
Rat MCCC1 shRNA Plasmid |
|||
| 20-abx988730 | Abbexa |
|
|
Mouse MCCC1 shRNA Plasmid |
|||
| 20-abx977639 | Abbexa |
|
|
Mouse Mccc1 ELISA KIT |
|||
| ELI-20526m | Lifescience Market | 96 Tests | EUR 865 |
Human MCCC1 ELISA KIT |
|||
| ELI-39149h | Lifescience Market | 96 Tests | EUR 824 |
MCCC1 ELISA KIT|Human |
|||
| EF000691 | Lifescience Market | 96 Tests | EUR 689 |
MCCC1 Recombinant Protein (Rat) |
|||
| RP211094 | ABM | 100 ug | Ask for price |
MCCC1 Recombinant Protein (Human) |
|||
| RP018955 | ABM | 100 ug | Ask for price |
MCCC1 Recombinant Protein (Mouse) |
|||
| RP149786 | ABM | 100 ug | Ask for price |
BCA Reagent, 16ML |
|||
| C144-16ML | Arbor Assays | 16ML | EUR 163 |
Dissociation Reagent, 1ML |
|||
| X017-1ML | Arbor Assays | 1ML | EUR 109 |
Dissociation Reagent, 25ML |
|||
| X017-25ML | Arbor Assays | 25ML | EUR 258 |
Dissociation Reagent, 5ML |
|||
| X017-5ML | Arbor Assays | 5ML | EUR 122 |
Dissociation Reagent, 1ML |
|||
| X058-1ML | Arbor Assays | 1ML | EUR 73 |
Dissociation Reagent, 5ML |
|||
| X058-5ML | Arbor Assays | 5ML | EUR 109 |
Biolipidure-1002-Reagent |
|||
| Biolipidure-1002-10 | Cusabio | 10mL | EUR 196 |
|
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
HighGene transfection reagent |
|||
| RM09014 | Abclonal | 1000μl | EUR 270 |
LP4K Transfection Reagent |
|||
| LP4K | GenTarget | 1.0 ml / vial | EUR 304 |
|
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types. |
|||
n-Heptane Reagent |
|||
| HC5400 | Bio Basic | 1L | EUR 79 |
Tri-RNA Reagent |
|||
| FATRR-001 | Favorgen | 100ml | EUR 236 |
Tri-RNA Reagent |
|||
| FATRR-002 | Favorgen | 50ml | EUR 176 |
Tri-RNA Reagent |
|||
| FATRR-003 | Favorgen | 450ml | EUR 645 |
DTT (Cleland's reagent) |
|||
| DB0058 | Bio Basic | 5g | EUR 84.8 |
DTNB (Ellman's Reagent) |
|||
| DB0113 | Bio Basic | 5g | EUR 97.85 |
Ethyl acetate Reagent |
|||
| EC4600 | Bio Basic | 1L | EUR 79 |
TissueDigest Reagent, 20X |
|||
| T101 | Insitus Biotechnologies | 10ml | EUR 210 |
Convoy? Transfection Reagent |
|||
| 1110-1ml | ACTGene | EUR 341 | |
Griess Reagent Kit |
|||
| 30100 | Biotium | 1KIT | EUR 149 |
|
Description: Minimum order quantity: 1 unit of 1KIT |
|||
PhosphoBlocker Blocking Reagent |
|||
| AKR-103 | Cell Biolabs | 1L | EUR 328 |
|
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
|||
PhosphoBlocker Blocking Reagent |
|||
| AKR-104 | Cell Biolabs | 4L | EUR 711 |
|
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
|||
EL Transfection Reagent |
|||
| 20-abx098880 | Abbexa |
|
|
Mycoplasma Prevention Reagent |
|||
| 20-abx098886 | Abbexa |
|
|
Girard's reagent T |
|||
| 20-abx184099 | Abbexa |
|
|
FcR blocking Reagent |
|||
| 20-abx290024 | Abbexa |
|
|
Detection Reagent A |
|||
| abx296004-120ul | Abbexa | 120 ul | EUR 321 |
Mycoplasma Prevention Reagent |
|||
| 20-abx298005 | Abbexa |
|
|
Biolipidure-1002-Reagent |
|||
| Biolipidure-1002-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-103-Reagent |
|||
| Biolipidure-103-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-103-Reagent |
|||
| Biolipidure-103-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-1201-Reagent |
|||
| Biolipidure-1201-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-1201-Reagent |
|||
| Biolipidure-1201-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-1301-Reagent |
|||
| Biolipidure-1301-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-1301-Reagent |
|||
| Biolipidure-1301-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-203-Reagent |
|||
| Biolipidure-203-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-203-Reagent |
|||
| Biolipidure-203-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-206-Reagent |
|||
| Biolipidure-206-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-206-Reagent |
|||
| Biolipidure-206-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-405-Reagent |
|||
| Biolipidure-405-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-405-Reagent |
|||
| Biolipidure-405-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-502-Reagent |
|||
| Biolipidure-502-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-502-Reagent |
|||
| Biolipidure-502-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-702-Reagent |
|||
| Biolipidure-702-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-702-Reagent |
|||
| Biolipidure-702-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-802-Reagent |
|||
| Biolipidure-802-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-802-Reagent |
|||
| Biolipidure-802-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Alcohol, Reagent (70%) |
|||
| EAS500 | ScyTek Laboratories | 500 ml | EUR 79 |
Alcohol, Reagent (70%) |
|||
| EAS999 | ScyTek Laboratories | 1000 ml | EUR 101 |
PureFection Transfection Reagent |
|||
| LV750A-1 | SBI | 1 ml | EUR 359 |
Biotin reagent (HRP) |
|||
| 65C-CE0202 | Fitzgerald | 5 mg | EUR 244 |
|
Description: HRP conjugated biotin labelling reagent |
|||
BSA (Reagent Grade) |
|||
| 30-AB79 | Fitzgerald | 1 kg | EUR 1552 |
|
Description: Reagent Grade Bovine Serum Albumin (99% pure) |
|||
BSA (Reagent Grade) |
|||
| 30-AB81 | Fitzgerald | 200 grams | EUR 476 |
|
Description: Reagent Grade Sulphydryl Blocked BSA (99% pure) |
|||
HAMA blocking reagent |
|||
| 85R-1001 | Fitzgerald | 1 gram | EUR 1974 |
|
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
|||
HAMA blocking reagent |
|||
| 85R-1001P | Fitzgerald | 1 gram | EUR 2190 |
|
Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
|||
HAMA blocking reagent |
|||
| 85R-1003 | Fitzgerald | 1 gram | EUR 1974 |
|
Description: HAMA Blocking Reagent for use in immunoassays such as Rapid Tests |
|||
HAMA blocking reagent |
|||
| 85R-1014 | Fitzgerald | 50 mg | EUR 192 |
|
Description: HAMA blocking reagent for use in assays specific for clinical false positive samples |
|||
HAMA blocking reagent |
|||
| 85R-1025 | Fitzgerald | 50 mg | EUR 192 |
|
Description: HAMA blocking reagent for use in immunoassays |
|||
HAMA blocking reagent |
|||
| 85R-1026 | Fitzgerald | 50 mg | EUR 192 |
|
Description: HAMA blocking reagent for use in immunoassays |
|||
Specimen Preservation Reagent |
|||
| DA0970 | Daan Gene | 100 test/kit | Ask for price |
Bradford Dye Reagent |
|||
| 0209R | AthenaES | 100 ml | EUR 131 |
BODIPY-Acetylene Reagent |
|||
| 2594-1 | Biovision | EUR 207 | |
BODIPY-Acetylene Reagent |
|||
| 2594-5 | Biovision | EUR 675 | |
ExFect2000 Transfection Reagent |
|||
| T202-01 | Vazyme | 0.5 ml | EUR 227 |
ExFect2000 Transfection Reagent |
|||
| T202-02 | Vazyme | 1 ml | EUR 316 |
ExFect2000 Transfection Reagent |
|||
| T202-03 | Vazyme | 5 ml | EUR 1052 |
Lung Lysate |
|||
| 1402 | ProSci | 0.1 mg | EUR 191 |
|
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Brain Lysate |
|||
| 1403 | ProSci | 0.1 mg | EUR 191 |
|
Description: Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Liver Lysate |
|||
| 1404 | ProSci | 0.1 mg | EUR 191 |
|
Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Kidney Lysate |
|||
| 1405 | ProSci | 0.1 mg | EUR 191 |
|
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Spleen Lysate |
|||
| 1406 | ProSci | 0.1 mg | EUR 191 |
|
Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Thymus Lysate |
|||
| 1409 | ProSci | 0.1 mg | EUR 191 |
|
Description: Thymus tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Bladder Lysate |
|||
| 1410 | ProSci | 0.1 mg | EUR 191 |
|
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Colon Lysate |
|||
| 1411 | ProSci | 0.1 mg | EUR 191 |
|
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Cerebellum Lysate |
|||
| 1412 | ProSci | 0.1 mg | EUR 191 |
|
Description: Cerebellum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Cerebrum Lysate |
|||
| 1413 | ProSci | 0.1 mg | EUR 191 |
|
Description: Cerebrum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Pancreas Lysate |
|||
| 1414 | ProSci | 0.1 mg | EUR 191 |
|
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
Stomach Lysate |
|||
| 1415 | ProSci | 0.1 mg | EUR 191 |
|
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
|||
An evaluation utilizing t-SNE to determine the emergence of PCs subclones by MFC, together with the evaluation of their immunophenotypic profiles are offered as a future perspective. Pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923) cultured for 18-24 h have been labeled by fluorescence probe carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), after which incubated with complete blood at 37℃. The phagocytosis of pathogens by neutrophils was detected by circulation cytometry, and a reference interval was established.