Plasma cell myeloma (a number of myeloma [MM]) is a malignant neoplasm originating from the plasma cells. Apart from different strategies, circulation cytometric evaluation of the affected person’s bone marrow aspirate has an vital function within the prognosis and in addition within the response evaluation. Because the cell floor markers, used for figuring out irregular plasma cells, are expressed diversely and the remedy also can alter the phenotype of the plasma cells, there may be an rising demand for brand spanking new plasma cell markers. VS38c is a monoclonal antibody that acknowledges the CLIMP-63 protein within the membrane of the endoplasmic reticulum.
CLIMP-63 is understood to be expressed at excessive ranges in regular and pathologic plasma cells within the bone marrow, thus VS38c antibody can be utilized to determine them. Though VS38c staining of plasma cells is reported to be fixed and robust even in myeloma, we have been questioning whether or not pattern preparation can have an effect on the staining. We’ve investigated the impact of various permeabilization brokers and washing of the cells on the standard of the VS38c staining and located that in lots of circumstances the staining is insufficient to determine the plasma cells.
We measured the VS38c staining of the bone marrow aspirates of 196 MM sufferers and noticed that the majority circumstances confirmed vivid staining with VS38c. Nonetheless, permeabilization with gentle detergent resulted within the look of a big VS38cdim subpopulation, which confirmed elevated sensitivity to mechanical stress (centrifugation). Our outcomes point out that VS38cdim MM cells can seem as a result of improper permeabilization of the endoplasmic reticulum and this discovering raises the opportunity of the existence of a plasma cell subpopulation with totally different membrane properties.
The importance of this inhabitants is unclear but, however these cells could be simply missed with VS38c staining and could be misplaced because of centrifugation-induced lysis throughout pattern preparation. Within the wholesome adults, the reference interval for the neutrophil phagocytosis to Escherichia coli was 46.91%-83.09% and to Staphylococcus aureus was 33.92%-69.48%. This methodology confirmed good reproducibility. Neutrophil phagocytosis was negatively correlated with the neutrophil depend, neutrophil share, and neutrophil-to-lymphocyte ratio
Quantification of Extracellular Double-stranded RNA Uptake and Subcellular Localization Utilizing Stream Cytometry and Confocal Microscopy
Double-stranded RNA is a potent pathogen-associated molecular sample (PAMP) produced as a by-product of viral replication and a well known hallmark of viral an infection. Viral dsRNAs could be launched from contaminated cells into the extracellular house and internalized by neighboring cells by way of endocytosis. Mammals possess a number of sample recognition receptors (PRRs) able to detecting viral dsRNAs reminiscent of endosomal toll-like receptor 3 (TLR3) and cytosolic RIG-I-like receptors (RLRs) which result in the manufacturing of kind I interferons (IFNs). Thus, intracellular localization of viral dsRNA can present perception into the downstream signaling pathways resulting in innate immune activation.
Right here, we describe a quantitative methodology for measuring extracellular dsRNA uptake and visualizing subcellular localization of internalized dsRNA by way of circulation cytometry and confocal microscopy respectively. The protocol described right here has been developed to detect RNA on the single cell degree. Fluorescent probes hybridize to focus on RNAs and are detected by circulation cytometry after a number of amplification steps. Several types of RNA could be detected reminiscent of mRNA, lengthy noncoding RNA, viral RNA or telomere RNA and as much as four totally different goal probes can be utilized concurrently. We used this protocol to particularly measure the expression of two transcription issue mRNAs, MAFB and IRF4, in human monocytes.
Analysis of measurable residual illness in a number of myeloma by multiparametric circulation cytometry: Present paradigm, pointers, and future purposes
A number of myeloma (MM) is a heterogeneous group of mature B-cell ailments which might be sometimes characterised by the presence and accumulation of irregular plasma cells (PCs), which ends up in the surplus manufacturing of monoclonal immunoglobulin and/or gentle chain discovered within the serum and/or urine. Multiparametric circulation cytometry (MFC) is an indispensable software to complement the prognosis, classification and monitoring of the illness because of its excessive affected person applicability, wonderful sensitivity and inspiring outcomes from numerous scientific trials.
On this regard, minimal or, extra appropriately, measurable residual illness (MRD) negativity by MFC has been acknowledged as a robust predictor of beneficial long-term outcomes. Earlier than circulation cytometry could be successfully carried out within the scientific setting for MM MRD testing, pattern preparation, panel configuration, evaluation and gating methods have to be optimized to make sure correct outcomes. This manuscript will talk about the present consensus pointers for circulation cytometric processing of samples and reporting of outcomes for MM MRD testing.
We additionally talk about various approaches to detect plasma cells within the presence of daratumumab remedy. Lastly, there’s a lack of awareness describing the subclonal distribution of myeloma cells based mostly on their protein expression. The arrival of high-dimensional evaluation might help in following the evolution of antigen expression patterns on irregular plasma cells in sufferers with relapsed/refractory illness. This in flip may also help determine clonal subtypes which might be extra aggressive for potential knowledgeable resolution.
 MCCC1 Antibody |
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| 1-CSB-PA013572GA01HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
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MCCC1 Antibody |
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| 47317-100ul | SAB | 100ul | EUR 302.4 |
 MCCC1 antibody |
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| 70R-18435 | Fitzgerald | 50 ul | EUR 522 |
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Description: Rabbit polyclonal MCCC1 antibody |
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 MCCC1 siRNA |
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| 20-abx903186 | Abbexa |
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MCCC1 siRNA |
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| 20-abx923705 | Abbexa |
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MCCC1 siRNA |
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| 20-abx923706 | Abbexa |
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 anti-MCCC1 |
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| YF-PA20071 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to MCCC1 |
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 Visitor Over Spectacle Clear |
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| SAF1410 | Scientific Laboratory Supplies | EACH | EUR 4.56 |
 MCCC1 Conjugated Antibody |
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| C47317 | SAB | 100ul | EUR 476.4 |
 MCCC1 cloning plasmid |
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| CSB-CL853497HU-10ug | Cusabio | 10ug | EUR 568.8 |
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Description: A cloning plasmid for the MCCC1 gene. |
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 anti- MCCC1 antibody |
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| FNab05047 | FN Test | 100µg | EUR 658.5 |
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Description: Antibody raised against MCCC1 |
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 MCCC1 Rabbit pAb |
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| A10020-100ul | Abclonal | 100 ul | EUR 369.6 |
MCCC1 Rabbit pAb |
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| A10020-200ul | Abclonal | 200 ul | EUR 550.8 |
MCCC1 Rabbit pAb |
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| A10020-20ul | Abclonal | 20 ul | EUR 219.6 |
MCCC1 Rabbit pAb |
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| A10020-50ul | Abclonal | 50 ul | EUR 267.6 |
 Anti-MCCC1 antibody |
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| PAab05047 | Lifescience Market | 100 ug | EUR 463.2 |
Anti-MCCC1 antibody |
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| STJ112060 | St John's Laboratory | 100 µl | EUR 332.4 |
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Description: This gene encodes the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. |
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) Anti-MCCC1 (2G8) |
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| YF-MA18990 | Abfrontier | 100 ug | EUR 435.6 |
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Description: Mouse monoclonal to MCCC1 |
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 Gas Change Over Unit 30Psi |
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| GAS1000 | Scientific Laboratory Supplies | EACH | EUR 904.02 |
 Gas Change Over Unit 60Psi |
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| GAS1002 | Scientific Laboratory Supplies | EACH | EUR 905.16 |
 Gas Change Over Unit 100Psi |
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| GAS1004 | Scientific Laboratory Supplies | EACH | EUR 922.26 |
 Automatic CO2 Change Over Unit |
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| INC6176 | Scientific Laboratory Supplies | EACH | EUR 1288.2 |
 Bolle TG10 Safety Over Glasses |
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| SAF1106 | Scientific Laboratory Supplies | EACH | EUR 8.39 |
 Forceps Dissecting Turn Over End |
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| D02129 | Scientific Laboratory Supplies | EACH | EUR 4.67 |
 EP Reagent Biuret Reagent |
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| 1011601 | Scientific Laboratory Supplies | 1L | EUR 42.18 |
 EP Reagent Iodoplatinate Reagent |
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| 1046300 | Scientific Laboratory Supplies | 200ML | EUR 360.24 |
 EP Reagent Methoxyphenylacetic Reagent |
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| 1053601 | Scientific Laboratory Supplies | 100ML | EUR 354.54 |
 EP Reagent Molybdovanadic Reagent |
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| 1056700 | Scientific Laboratory Supplies | 100ML | EUR 42.18 |
 EP Reagent Phosphomolybdotungstic Reagent |
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| 1065000 | Scientific Laboratory Supplies | 100ML | EUR 161.88 |
 Human MCCC1 shRNA Plasmid |
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| 20-abx961224 | Abbexa |
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 Rat MCCC1 shRNA Plasmid |
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| 20-abx988730 | Abbexa |
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 Mouse MCCC1 shRNA Plasmid |
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| 20-abx977639 | Abbexa |
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 MCCC1 Antibody, HRP conjugated |
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| 1-CSB-PA853497EB01HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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 MCCC1 Antibody, FITC conjugated |
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| 1-CSB-PA853497EC01HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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 MCCC1 Antibody, Biotin conjugated |
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| 1-CSB-PA853497ED01HU | Cusabio |
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Description: A polyclonal antibody against MCCC1. Recognizes MCCC1 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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 Human MCCC1 ELISA KIT |
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| ELI-39149h | Lifescience Market | 96 Tests | EUR 988.8 |
 Mouse Mccc1 ELISA KIT |
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| ELI-20526m | Lifescience Market | 96 Tests | EUR 1038 |
 MCCC1 ELISA KIT|Human |
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| EF000691 | Lifescience Market | 96 Tests | EUR 826.8 |
) MCCC1 Recombinant Protein (Human) |
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| RP018955 | ABM | 100 ug | Ask for price |
) MCCC1 Recombinant Protein (Rat) |
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| RP211094 | ABM | 100 ug | Ask for price |
) MCCC1 Recombinant Protein (Mouse) |
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| RP149786 | ABM | 100 ug | Ask for price |
 DURAN Over-Cap 45mm Black Phenolic |
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| BOT2596 | Scientific Laboratory Supplies | PK10 | EUR 25.08 |
 EP Reagent Sulfomolybdic Reagent R3 |
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| 1086500 | Scientific Laboratory Supplies | 1L | EUR 287.28 |
 Bluing Reagent |
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| BRT030 | ScyTek Laboratories | 30 ml | EUR 72 |
Bluing Reagent |
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| BRT125 | ScyTek Laboratories | 125 ml | EUR 75.6 |
Bluing Reagent |
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| BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 220.8 |
Bluing Reagent |
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| BRT500 | ScyTek Laboratories | 500 ml | EUR 91.2 |
Bluing Reagent |
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| BRT999 | ScyTek Laboratories | 1000 ml | EUR 105.6 |
 BOP reagent |
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| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | Ask for price |
 Chymase reagent |
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| 30C-CP1129 | Fitzgerald | 5 units | EUR 2622 |
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Description: Purified native Human Chymase reagent |
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BOP reagent |
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| A7015-100000 | ApexBio | 100 g | EUR 240 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | EUR 135.6 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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 Bradford reagent |
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| BDE641 | Bio Basic | 100ml | EUR 73.21 |
 Beaucage reagent |
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| HY-100951 | MedChemExpress | 10mM/1mL | EUR 151.2 |
 Ninhydrin Reagent |
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| MIC6746 | Scientific Laboratory Supplies | EACH | EUR 31.12 |
 Nessler Reagent |
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| NESSR | Scientific Laboratory Supplies | 500ML | EUR 148.2 |
 Phosphate Reagent |
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| 106199 | Scientific Laboratory Supplies | PK100 | EUR 61.29 |
 Chromium Reagent |
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| 1206699 | Scientific Laboratory Supplies | EACH | EUR 141.72 |
 Thioacetamide Reagent |
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| THIOR01 | Scientific Laboratory Supplies | 100ML | EUR 78.66 |
 MTS Reagent |
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| 2808-1000 | Biovision | each | EUR 1188 |
MTS Reagent |
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| 2808-250 | Biovision | each | EUR 438 |
 MTT Reagent |
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| 2809-1G | Biovision | each | EUR 216 |
MTT Reagent |
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| 2809-5G | Biovision | each | EUR 652.8 |
 Traut's Reagent |
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| 2330-1000 | Biovision | each | EUR 418.8 |
Traut's Reagent |
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| 2330-500 | Biovision | each | EUR 248.4 |
 HEK-293T Telomerase Over-Expressing Cell Pellet |
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| abx069991-1Pellet | Abbexa | 1 Pellet | EUR 477.6 |
 Visitor Eye Shield Over Specs - Portwest PW30 |
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| SAF1021 | Scientific Laboratory Supplies | EACH | EUR 3.42 |
 (OKCA00723)) MCCC1 ELISA Kit (Human) (OKCA00723) |
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| OKCA00723 | Aviva Systems Biology | 96 Wells | EUR 999.6 |
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Description: Description of target: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 5.8 pg/mL |
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 (pORF)) Mccc1 ORF Vector (Rat) (pORF) |
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| ORF070366 | ABM | 1.0 ug DNA | EUR 607.2 |
 (pORF)) MCCC1 ORF Vector (Human) (pORF) |
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| ORF006319 | ABM | 1.0 ug DNA | EUR 114 |
 (pORF)) Mccc1 ORF Vector (Mouse) (pORF) |
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| ORF049930 | ABM | 1.0 ug DNA | EUR 607.2 |
 Kidney Lysate |
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| 21-104 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Bovine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Liver Lysate |
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| 21-105 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Bovine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Heart Lysate |
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| 21-115 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Guinea Pig heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig heart tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Kidney Lysate |
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| 21-116 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Guinea Pig kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Adrenal Lysate |
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| 21-160 | ProSci | 0.1 mg | EUR 468.6 |
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Description: Monkey (Cynomolgus) adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) adrenal tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Colon Lysate |
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| 21-179 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Cynomolgus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Gallbladder Lysate |
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| 21-188 | ProSci | 0.1 mg | EUR 468.6 |
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Description: Monkey (Cynomolgus) gallbladder tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) gallbladder tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the gallbladder tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The gallbladder tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Kidney Lysate |
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| 21-190 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Cynomolgus) kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Lung Lysate |
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| 21-194 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Cynomolgus) lung tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) lung tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lung tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lung tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Skin Lysate |
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| 21-204 | ProSci | 0.1 mg | EUR 468.6 |
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Description: Monkey (Cynomolgus) skin tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skin tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skin tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skin tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Spleen Lysate |
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| 21-209 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Cynomolgus) spleen tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) spleen tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the spleen tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The spleen tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Brain Lysate |
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| 21-272 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Rhesus) brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Colon Lysate |
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| 21-288 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Rhesus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Gallbladder Lysate |
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| 21-298 | ProSci | 0.1 mg | EUR 468.6 |
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Description: Monkey (Rhesus) gallbladder tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) gallbladder tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the gallbladder tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The gallbladder tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Heart Lysate |
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| 21-299 | ProSci | 0.1 mg | EUR 468.6 |
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Description: Monkey (Rhesus) heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) heart tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Lung Lysate |
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| 21-305 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Monkey (Rhesus) lung tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) lung tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lung tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lung tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Skin Lysate |
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| 21-315 | ProSci | 0.1 mg | EUR 468.6 |
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Description: Monkey (Rhesus) skin tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) skin tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skin tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skin tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Placenta Lysate |
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| 21-393 | ProSci | 0.1 mg | EUR 500.1 |
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Description: Mouse placenta tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The mouse placenta tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the placenta tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The placenta tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Rectum Lysate |
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| 21-394 | ProSci | 0.1 mg | EUR 663.9 |
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Description: Mouse rectum tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The mouse rectum tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the rectum tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The rectum tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Heart Lysate |
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| 21-404 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Porcine heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The porcine heart tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Kidney Lysate |
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| 21-405 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Porcine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The porcine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Liver Lysate |
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| 21-406 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Porcine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The porcine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Brain Lysate |
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| 21-414 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Rabbit brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Kidney Lysate |
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| 21-418 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Rabbit kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Liver Lysate |
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| 21-419 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Rabbit liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Lung Lysate |
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| 21-420 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Rabbit lung tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rabbit lung tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lung tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lung tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Ovary Lysate |
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| 21-468 | ProSci | 0.1 mg | EUR 468.6 |
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Description: Rat ovary tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rat ovary tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the ovary tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The ovary tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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Placenta Lysate |
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| 21-469 | ProSci | 0.1 mg | EUR 342.6 |
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Description: Rat placenta tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rat placenta tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the placenta tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The placenta tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
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 Esophagus Lysate |
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| 1365 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Esophagus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Ileum Lysate |
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| 1367 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Ileum tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Rectum Lysate |
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| 1373 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Rectum tissue lysate was prepared by homogenization in homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Skin Lysate |
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| 1376 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Skin tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Thyroid Lysate |
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| 1380 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Thyroid tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Spleen Lysate |
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| 1406 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Bladder Lysate |
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| 1410 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Cerebellum Lysate |
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| 1412 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Cerebellum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Cerebrum Lysate |
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| 1413 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Cerebrum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Pancreas Lysate |
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| 1414 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Stomach Lysate |
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| 1415 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Testis Lysate |
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| 1416 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Adrenal Lysate |
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| 1417 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Skin Lysate |
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| 1419 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Skin tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Eye Lysate |
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| 1420 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Eye tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Trachea Lysate |
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| 1422 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Trachea tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Lung Lysate |
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| 1462 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Liver Lysate |
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| 1464 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Kidney Lysate |
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| 1465 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Spleen Lysate |
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| 1466 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Pancreas Lysate |
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| 1469 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Adrenal Lysate |
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| 1470 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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 Thymus Lysate |
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| 1471 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Thymus tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Colon Lysate |
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| 1472 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Cerebellum Lysate |
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| 1473 | ProSci | 0.1 mg | EUR 229.2 |
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Description: Cerebellum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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An evaluation utilizing t-SNE to determine the emergence of PCs subclones by MFC, together with the evaluation of their immunophenotypic profiles are offered as a future perspective. Pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923) cultured for 18-24 h have been labeled by fluorescence probe carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), after which incubated with complete blood at 37℃. The phagocytosis of pathogens by neutrophils was detected by circulation cytometry, and a reference interval was established.