Limitations of VS38c labeling in the detection of plasma cell myeloma by flow cytometry
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Limitations of VS38c labeling in the detection of plasma cell myeloma by flow cytometry

- August 21, 2021 - Dominique

Plasma cell myeloma (a number of myeloma [MM]) is a malignant neoplasm originating from the plasma cells. Apart from different strategies, circulation cytometric evaluation of the affected person’s bone marrow aspirate has an vital function within the prognosis and in addition within the response evaluation. Because the cell floor markers, used for figuring out irregular plasma cells, are expressed diversely and the remedy also can alter the phenotype of the plasma cells, there may be an rising demand for brand spanking new plasma cell markers. VS38c is a monoclonal antibody that acknowledges the CLIMP-63 protein within the membrane of the endoplasmic reticulum.

CLIMP-63 is understood to be expressed at excessive ranges in regular and pathologic plasma cells within the bone marrow, thus VS38c antibody can be utilized to determine them. Though VS38c staining of plasma cells is reported to be fixed and robust even in myeloma, we have been questioning whether or not pattern preparation can have an effect on the staining. We’ve investigated the impact of various permeabilization brokers and washing of the cells on the standard of the VS38c staining and located that in lots of circumstances the staining is insufficient to determine the plasma cells.

We measured the VS38c staining of the bone marrow aspirates of 196 MM sufferers and noticed that the majority circumstances confirmed vivid staining with VS38c. Nonetheless, permeabilization with gentle detergent resulted within the look of a big VS38cdim subpopulation, which confirmed elevated sensitivity to mechanical stress (centrifugation). Our outcomes point out that VS38cdim MM cells can seem as a result of improper permeabilization of the endoplasmic reticulum and this discovering raises the opportunity of the existence of a plasma cell subpopulation with totally different membrane properties.

The importance of this inhabitants is unclear but, however these cells could be simply missed with VS38c staining and could be misplaced because of centrifugation-induced lysis throughout pattern preparation. Within the wholesome adults, the reference interval for the neutrophil phagocytosis to Escherichia coli was 46.91%-83.09% and to Staphylococcus aureus was 33.92%-69.48%. This methodology confirmed good reproducibility. Neutrophil phagocytosis was negatively correlated with the neutrophil depend, neutrophil share, and neutrophil-to-lymphocyte ratio

Quantification of Extracellular Double-stranded RNA Uptake and Subcellular Localization Utilizing Stream Cytometry and Confocal Microscopy

Double-stranded RNA is a potent pathogen-associated molecular sample (PAMP) produced as a by-product of viral replication and a well known hallmark of viral an infection. Viral dsRNAs could be launched from contaminated cells into the extracellular house and internalized by neighboring cells by way of endocytosis. Mammals possess a number of sample recognition receptors (PRRs) able to detecting viral dsRNAs reminiscent of endosomal toll-like receptor 3 (TLR3) and cytosolic RIG-I-like receptors (RLRs) which result in the manufacturing of kind I interferons (IFNs). Thus, intracellular localization of viral dsRNA can present perception into the downstream signaling pathways resulting in innate immune activation.

Right here, we describe a quantitative methodology for measuring extracellular dsRNA uptake and visualizing subcellular localization of internalized dsRNA by way of circulation cytometry and confocal microscopy respectively. The protocol described right here has been developed to detect RNA on the single cell degree. Fluorescent probes hybridize to focus on RNAs and are detected by circulation cytometry after a number of amplification steps. Several types of RNA could be detected reminiscent of mRNA, lengthy noncoding RNA, viral RNA or telomere RNA and as much as four totally different goal probes can be utilized concurrently. We used this protocol to particularly measure the expression of two transcription issue mRNAs, MAFB and IRF4, in human monocytes.

Limitations of VS38c labeling in the detection of plasma cell myeloma by flow cytometry

Analysis of measurable residual illness in a number of myeloma by multiparametric circulation cytometry: Present paradigm, pointers, and future purposes

A number of myeloma (MM) is a heterogeneous group of mature B-cell ailments which might be sometimes characterised by the presence and accumulation of irregular plasma cells (PCs), which ends up in the surplus manufacturing of monoclonal immunoglobulin and/or gentle chain discovered within the serum and/or urine. Multiparametric circulation cytometry (MFC) is an indispensable software to complement the prognosis, classification and monitoring of the illness because of its excessive affected person applicability, wonderful sensitivity and inspiring outcomes from numerous scientific trials.

On this regard, minimal or, extra appropriately, measurable residual illness (MRD) negativity by MFC has been acknowledged as a robust predictor of beneficial long-term outcomes. Earlier than circulation cytometry could be successfully carried out within the scientific setting for MM MRD testing, pattern preparation, panel configuration, evaluation and gating methods have to be optimized to make sure correct outcomes. This manuscript will talk about the present consensus pointers for circulation cytometric processing of samples and reporting of outcomes for MM MRD testing.

We additionally talk about various approaches to detect plasma cells within the presence of daratumumab remedy. Lastly, there’s a lack of awareness describing the subclonal distribution of myeloma cells based mostly on their protein expression. The arrival of high-dimensional evaluation might help in following the evolution of antigen expression patterns on irregular plasma cells in sufferers with relapsed/refractory illness. This in flip may also help determine clonal subtypes which might be extra aggressive for potential knowledgeable resolution.

293 Expression MAX-1
EXP-711-11 1ml
EUR 214
Description: 293 Expression MAX-1 is expressed from - -.

CNGA2 Expression vector
60642 20 µg
EUR 900
Description: The CNGA2 expression vector is designed to express human cyclic nucleotide-gated cation channel 2 (CNGA2) in mammalian cells.

pYLEX1 - Expression Vector
FYY203-5MG 5mg Ask for price

RFP expression Adenovirus, in vivo ready
AVP001-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made RFP expression adenovirus, provided in PBS solution.

CFP expression Adenovirus, in vivo ready
AVP002-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made CFP expression adenovirus, provided in PBS solution.

GFP expression Adenovirus, in vivo ready
AVP011-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made GFP expression adenovirus, provided in PBS solution.

YFP expression Adenovirus, in vivo ready
AVP012-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made YFP expression adenovirus, provided in PBS solution.

BFP expression Adenovirus, in vivo ready
AVP017-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made BFP expression adenovirus, provided in PBS solution.

Blunt E1 Expression Kit
abx098098-10rxns 10 rxns
EUR 560.4

Blunt E2 Expression Kit
abx098873-10rxns 10 rxns
EUR 560.4

RFP expression Adenovirus
AVP001 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made RFP expression adenovirus, provided in DMEM medium.

CFP expression Adenovirus
AVP002 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made CFP expression adenovirus, provided in DMEM medium.

GFP expression Adenovirus
AVP011 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made GFP expression adenovirus, provided in DMEM medium.

YFP expression Adenovirus
AVP012 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made YFP expression adenovirus, provided in DMEM medium.

BFP expression Adenovirus
AVP017 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made BFP expression adenovirus, provided in DMEM medium.

Rac1 Expression Vector Set
STA-454 1 kit
EUR 1765.2
Description: Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and G12V constitutively active mutant.

RhoA Expression Vector Set
STA-456 1 kit
EUR 1765.2
Description: RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and G14V constitutively active mutant.

h OCT4 expression Adenovirus, in vivo ready
AVP013-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in PBS solution for in vivo application.

h SOX2 expression Adenovirus, in vivo ready
AVP014-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in PBS solution for in vivo application.

Cdc42 Expression Vector Set
STA-455 1 kit
EUR 1765.2
Description: Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant.

h LIN28 expression Adenovirus, in vivo ready
AVP015-PBS 1x1011 IFU/ml x 50ul
EUR 852
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in PBS solution for in vivo application.

h ADA Expression Lentivirus
LVP923 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ADA, containing a RFP-Blasticidin dual selection marker.

h IL6 Expression Lentivirus
LVP1144 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD4 Expression Lentivirus
LVP1152 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD4, containing a RFP-Blasticidin dual selection marker.

h IL4 Expression Lentivirus
LVP1161 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL4, containing a RFP-Blasticidin dual selection marker.

h IL7 Expression Lentivirus
LVP1162 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KDR Expression Lentivirus
LVP1176 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KDR, containing a RFP-Blasticidin dual selection marker.

h FAS Expression Lentivirus
LVP1181 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: FAS, containing a RFP-Blasticidin dual selection marker.

pAAV-MCS Expression Vector
VPK-410 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

h OCT4 expression Adenovirus
AVP013 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, OCT4, provided in DMEM medium.

h SOX2 expression Adenovirus
AVP014 1x109 IFU/ml x 50ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, SOX2, provided in DMEM medium.

h CDR1 Expression Lentivirus
LVP917 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CDR1, containing a RFP-Blasticidin dual selection marker.

h CD47 Expression Lentivirus
LVP918 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD47, containing a RFP-Blasticidin dual selection marker.

h ESR1 Expression Lentivirus
LVP924 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ESR1, containing a RFP-Blasticidin dual selection marker.

r Syn1 Expression Lentivirus
LVP925 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for rat target: Syn1, containing a RFP-Blasticidin dual selection marker.

h NME4 Expression Lentivirus
LVP932 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NME4, containing a RFP-Blasticidin dual selection marker.

h EGFR Expression Lentivirus
LVP1142 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EGFR, containing a RFP-Blasticidin dual selection marker.

h NRP1 Expression Lentivirus
LVP1145 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h MUC1 Expression Lentivirus
LVP1148 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h CD8a Expression Lentivirus
LVP1151 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CD8a, containing a RFP-Blasticidin dual selection marker.

h PIGF Expression Lentivirus
LVP1154 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PIGF, containing a RFP-Blasticidin dual selection marker.

h BRAF Expression Lentivirus
LVP1156 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: BRAF, containing a RFP-Blasticidin dual selection marker.

h ROS1 Expression Lentivirus
LVP1158 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ROS1, containing a RFP-Blasticidin dual selection marker.

h CD3E Expression Lentivirus
LVP1163 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL7, containing a RFP-Blasticidin dual selection marker.

h KLK3 Expression Lentivirus
LVP1171 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KLK3, containing a RFP-Blasticidin dual selection marker.

h NEK6 Expression Lentivirus
LVP1182 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK6, containing a RFP-Blasticidin dual selection marker.

h NEK7 Expression Lentivirus
LVP1183 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NEK7, containing a RFP-Blasticidin dual selection marker.

h LIN28 expression Adenovirus
AVP015 1x109 IFU/ml x 200ul
EUR 418.8
Description: pre-made adenovirus express human stem cell factor, LIN28, provided in DMEM medium.

h GPNMB Expression Lentivirus
LVP921 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: GPNMB, containing a RFP-Blasticidin dual selection marker.

h DECR1 Expression Lentivirus
LVP927 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR1, containing a RFP-Blasticidin dual selection marker.

h DECR2 Expression Lentivirus
LVP928 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: DECR2, containing a RFP-Blasticidin dual selection marker.

h NMRK1 Expression Lentivirus
LVP930 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NMRK1, containing a RFP-Blasticidin dual selection marker.

h PITX3 Expression Lentivirus
LVP931 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PITX3, containing a RFP-Blasticidin dual selection marker.

h HDAC1 Expression Lentivirus
LVP1143 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h VEGFB Expression Lentivirus
LVP1146 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h PARP1 Expression Lentivirus
LVP1149 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h IL3RA Expression Lentivirus
LVP1150 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL3RA, containing a RFP-Blasticidin dual selection marker.

h PTCH1 Expression Lentivirus
LVP1155 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PTCH1, containing a RFP-Blasticidin dual selection marker.

h SART3 Expression Lentivirus
LVP1159 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SART3, containing a RFP-Blasticidin dual selection marker.

h TIGIT Expression Lentivirus
LVP1160 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TIGIT, containing a RFP-Blasticidin dual selection marker.

h EDNRA Expression Lentivirus
LVP1165 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: EDNRA containing a RFP-Blasticidin dual selection marker.

h Foxp3 Expression Lentivirus
LVP1172 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: Foxp3, containing a RFP-Blasticidin dual selection marker.

m RIKEN Expression Lentivirus
LVP1174 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for mouse target: RIKEN, containing a RFP-Blasticidin dual selection marker.

h APLP2 Expression Lentivirus
LVP1177 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: APLP2, containing a RFP-Blasticidin dual selection marker.

h PCSK9 Expression Lentivirus
LVP1178 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: PCSK9, containing a RFP-Blasticidin dual selection marker.

h NTSR1 Expression Lentivirus
LVP1179 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NTSR1, containing a RFP-Blasticidin dual selection marker.

pscAAV-MCS Expression Vector
VPK-430 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

H-Ras Expression Vector Set
STA-457 1 kit
EUR 1765.2
Description: H-Ras Expression Vector Set contains 3 vectors: H-Ras wild type, T19N dominant negative mutant, and G12V constitutively active mutant.

m CTAGA1 Expression Lentivirus
LVP916 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for mouse target: CTAGA1, containing a RFP-Blasticidin dual selection marker.

h SKIV2L Expression Lentivirus
LVP919 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a RFP-Blasticidin dual selection marker.

h SKIV2L Expression Lentivirus
LVP919-GP 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: SKIV2L, containing a GFP-Puromycin dual selection marker.

h TP5313 Expression Lentivirus
LVP926 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TP5313, containing a RFP-Blasticidin dual selection marker.

h ELOVL5 Expression Lentivirus
LVP929 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: ELOVL5, containing a RFP-Blasticidin dual selection marker.

h MAGEA3 Expression Lentivirus
LVP1147 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: HDAC1, containing a RFP-Blasticidin dual selection marker.

h NANOS3 Expression Lentivirus
LVP1164 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: NANOS3, containing a RFP-Blasticidin dual selection marker.

h IL1RL1 Expression Lentivirus
LVP1168 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: IL1RL1, containing a RFP-Blasticidin dual selection marker.

h CTAG1B Expression Lentivirus
LVP1169 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CTAG1B, containing a RFP-Blasticidin dual selection marker.

h MAGEA3 Expression Lentivirus
LVP1170 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: MAGEA3, containing a RFP-Blasticidin dual selection marker.

h COX7A1 Expression Lentivirus
LVP1173 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: COX7A1, containing a RFP-Blasticidin dual selection marker.

h BCL11B Expression Lentivirus
LVP1180 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: BCL11B, containing a RFP-Blasticidin dual selection marker.

pUC57-sgRNA expression vector
PVT18003 2 ug
EUR 309.6

BRAF, His-Tag (High Expression)
41237 10 µg
EUR 375
Description: Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa.

ANPRA/Aequorin Expression vector
60641 20 µg
EUR 900
Description: The ANPRA/Aequorin expression vector is designed to co-express human atrial natriuretic peptide receptor A (ANPRA, also called natriuretic peptide receptor A/guanylate cyclase A) and jellyfish (Aequorea victoria) Aequorin in mammalian cells.

h CEACAM5 Expression Lentivirus
LVP1153 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: CEACAM5, containing a RFP-Blasticidin dual selection marker.

h TNFSF11 Expression Lentivirus
LVP1157 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TNFSF11, containing a RFP-Blasticidin dual selection marker.

h TNFRSF1B Expression Lentivirus
LVP922 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: TNFRSF1B, containing a RFP-Blasticidin dual selection marker.

h KIAA1324 Expression Lentivirus
LVP1175 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KIAA1324, containing a RFP-Blasticidin dual selection marker.

GFP-Rac1 Expression Vector Set
STA-450 1 kit
EUR 1939.2
Description: GFP-Rac1 Expression Vector Set contains 3 vectors: Rac wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

GFP-RhoA Expression Vector Set
STA-452 1 kit
EUR 1939.2
Description: GFP-RhoA Expression Vector Set contains 3 vectors: RhoA wild type, T19N dominant negative mutant, and Q63L constitutively active mutant. Each vector also contains a GFP reporter sequence.

h KIAA0319L Expression Lentivirus
LVP920 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for human target: KIAA0319L, containing a RFP-Blasticidin dual selection marker.

GFP-Cdc42 Expression Vector Set
STA-451 1 kit
EUR 1939.2
Description: GFP-Cdc42 Expression Vector Set contains 3 vectors: Cdc42 wild type, T17N dominant negative mutant, and Q61L constitutively active mutant. Each vector also contains a GFP reporter sequence.

Active Rac1 Expression Vector Set
STA-458 1 kit
EUR 1765.2
Description: Active Rac1 Expression Vector Set contains 3 vectors expressing different constitutively active mutants of Rac1: Q61L, Q61L/F37A, and Q61L/Y40C.

PhiC31 Integrase Expression Plasmid
FC200PA-1 10ug
EUR 496

pCMV-GFP-LC3 Expression Vector
CBA-401 100 µL
EUR 1243.2
Description: Expression vector contains a fusion of GFP and LC3. A separate GFP control vector is also included.

h KRAS (G12D) Expression Lentivirus
LVP1166 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for a mutant (G12D) of human target: KRAS containing a RFP-Blasticidin dual selection marker.

Tet-on circRNA Expression Vector
PVT14643 2 ug
EUR 843.6

h TP53 (R175H) Expression Lentivirus
LVP1167 1x107 IFU/ml x 200ul
EUR 541.2
Description: Pre-made over-expression lentivirus for a mutant R175H) of human target: TP53, containing a RFP-Blasticidin dual selection marker.

pAAV-IRES-Neo Expression Vector
VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector
VPK-418 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Bsd Expression Vector
VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

PinPoint Integrase Expression Plasmid
PIN200A-1 10ug
EUR 688

pAAV-IRES-Puro Expression Vector
VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

CEACAM5 CHO Cell Line (Low Expression)
78704-L 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

Active H-Ras Expression Vector Set
STA-459 1 kit
EUR 1765.2
Description: Active H-Ras Expression Vector Set contains 3 vectors expressing different constitutively active mutants of H-Ras: V12, V12S35, and V12C40.

B7-H3 CHO Cell Line (Low Expression)
78541-L 2 vials
EUR 7150
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.

pAAV-IRES-Hygro Expression Vector
VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

CD36 HEK293 Cell Line (High Expression)
78526-H 2 vials
EUR 7150
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line.

CEACAM5 CHO Cell Line (High Expression)
78704-H 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

AAV-1 Helper Free Expression System
VPK-410-SER1 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-2 Helper Free Expression System
VPK-410-SER2 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-3 Helper Free Expression System
VPK-410-SER3 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-4 Helper Free Expression System
VPK-410-SER4 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-5 Helper Free Expression System
VPK-410-SER5 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

AAV-6 Helper Free Expression System
VPK-410-SER6 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

B7-H3 CHO Cell Line (High Expression)
78541-H 2 vials
EUR 7150
Description: B7 homolog 3 protein (B7-H3) CHO Cell line is a recombinant clonal stable CHO cell line expressing full length human B7-H3 protein, also known as cluster of differentiation 276 (CD276, B7H3; B7RP-2; 4Ig-B7-H3). Surface expression of B7-H3 was confirmed by flow cytometry. Each stable clonal cell line was selected for High, Medium, or Low levels of B7-H3 expression to mimic different B7-H3 expression levels in various cancer types.

AAV-DJ Helper Free Expression System
VPK-410-DJ 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

Gryphon Retroviral Expression System I
ABP-RVK-10001 1 set Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

Gryphon Retroviral Expression System II
ABP-RVK-10002 1 set Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

CD7 CHO Cell Line (Medium or High Expression)-78324-H
78324-H 2 vials
EUR 7150
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels.

CD7 CHO Cell Line (Medium or High Expression)-78324-M
78324-M 2 vials
EUR 7150
Description: Recombinant CD7 CHO-K1 cell lines expressing full-length human CD7 receptor (accession number: NM_006137.6). Surface expression of human CD7 was confirmed by flow cytometry. Each stable clonal cell line was selected for high or medium levels of CD7 expression to mimic different stages of cancer target cells with various CD7 expression levels.

CD36 HEK293 Cell Line (Medium Expression)
78526-M 2 vials
EUR 7150
Description: Recombinant clonal HEK293 cell line stably expressing full length human CD36. The stable clonal cell line was selected for medium and high levels of CD36 expression compared to the parental HEK293 cell line.

CEACAM5 CHO Cell Line (Medium Expression)
78704-M 2 vials
EUR 7150
Description: Recombinant clonal CHO cell line stably expressing full-length human CEACAM5 (NM_004363). Surface expression of CEACAM5 was confirmed by flow cytometry. The stable clonal cell line was selected for different levels of CEACAM5 expression (High, Medium, and Low) compared to the parental CHO-K1 cell line.

Human Myelin expression factor 2 (MYEF2)
1-CSB-EP015283HU
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Myelin expression factor 2(MYEF2),partial expressed in E.coli

scAAV-1 Helper Free Expression System
VPK-430-SER1 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

scAAV-2 Helper Free Expression System
VPK-430-SER2 1 kit
EUR 1486.8
Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

An evaluation utilizing t-SNE to determine the emergence of PCs subclones by MFC, together with the evaluation of their immunophenotypic profiles are offered as a future perspective. Pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923) cultured for 18-24 h have been labeled by fluorescence probe carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), after which incubated with complete blood at 37℃. The phagocytosis of pathogens by neutrophils was detected by circulation cytometry, and a reference interval was established.

 

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